Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
20 Cards in this Set
- Front
- Back
VonKossa
|
Stain for calcium deposits
stains black |
|
Alizarin red
|
Stain for calcium deposits
stains red |
|
What are the stains used for calcium deposits?
|
VonKossa (black)
Alizarin red (red) |
|
What are the the stains for Amyloid?
|
Lugol’s iodide
enhanced w/ sulfuric acid (gross) Congo red (histo) Potassium permanganate (histo) PAS (Periodic Acid Schiff) (histo) |
|
Lugol’s iodide
enhanced w/ sulfuric acid |
Gross staining for presence of amyloid
Lugol’s alone brown-black, w/ sulfuric acid, blue-black |
|
Congo red
|
histological stain for amyloid
Pink to red w/ apple green birefringence |
|
Potassium permanganate
|
histological stain for amyloid
Lose affinity for Congo red DIIFERENTIATE b/t primary and secondary by incubating tissue w/ potassium permanganate – secondary (AA protein) will lose affinity for Congo red |
|
PAS (Periodic Acid Schiff)
|
histological stain for amyloid and glucose
pink Works because there is some amount of carbohydrate in amyloid Unstained forms clear vacuoles with irregular borders (glucose) |
|
Best Carmine
|
stain for glucose
pink unstained forms clear vacuoles with irregular borders |
|
What are the stains for glucose?
|
PAS (Periodic Acid Schiff)
Best carmine |
|
What are the stains for lipids?
|
Sudan Red (red)
Oil red O (red) Osmic Acid (black) |
|
Sudan red
|
histological stain for lipids
red Vacuoles sharply demarcated |
|
Oil red O
|
histological stain for lipids
red Vacuoles sharply demarcated |
|
Osmic Acid
|
Histological stain for lipid
black vacuoles sharply demarcated |
|
What is Anthracosis?
|
presence of carbon particles in the lung
Is not stained, but can be seen as a deposit in lungs (seen in cigarette smokers) |
|
What is Lipofuscin?
|
Associated with “wear and tear” – esp. in permanent cells (cardiac & neurons)
brown and granular Autoflourescent and acid-fast Antemortem |
|
Prussian blue
|
Stain for hemosiderin
Blue-black presence of iron |
|
Masson- Fontana
|
stain for melanin
black background with white nuclei |
|
Phosphotungstic acid hematoxylin (PTAH)
|
Stains fibrin
black |
|
What does thrichome stain?
|
fibrosis
|