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130 Cards in this Set

  • Front
  • Back

Fasciola hepatica (liver fluke of sheep)

Fasciola hepatica (liver fluke of sheep)

Diphyllobothrium latum (broad fish tapeworm)



Look very similar to fluke eggs, but less golden in color

Diphyllobothrium latum (broad fish tapeworm)



Look very similar to fluke eggs, but less golden in color

Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)



Look at them larval hooks!

Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

This is typical of which parasite(s)?

Cestodes - Taenia or Echinococcus


Notice the thick wall and larval hooks.

Echinococcus granulosus (tapeworm)

Echinococcus granulosus (tapeworm)

Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

Diphylidium caninum (dog/flea tape worm)

Diphylidium caninum (dog/flea tape worm)

Fasciola hepatica (liver fluke of sheep)

Moniezia (tape worm of sheep & other ruminants)



Looks like an ice cube

Moniezia (tape worm of sheep & other ruminants)



Sort of ice cube-ish

Baylisascaris procyonis (racoon roundworm)

Toxocara canis (dog roundworm)

Trichuris trichiura (human whipworm)

Trichuris trichiura (human whipworm)

General hookworm egg

A: Nematodirus spp.


B: General strongyle egg



Nematodirus is HUGE. That's why we can differentiate it from the other strongly eggs (roundworms).

Nematodirus spp. (roundworm of sheep)

Strongyle eggs (roundworms)



Differentiation of different roundworm species is difficult and we won't be expected to do so EXCEPT for Nematodirus which is easy to recognize because it is huge (clearly visible on 10x).

Ascaris spp. (roundworm)

Ascaris (roundworm)

What is going on here?

This shows the development of ascaris eggs



1. Umembryonated egg from fresh feces


2. Unfertilized egg (left) and embryonating egg (right) in older feces


3. Egg with larva from even older feces - look for this when identifying!

Ancylostoma caninum (dog hookworm)

Ancylostoma caninum (dog hookworm)

Ancylostoma caninum (dog hookworm)

Parascaris spp. (roundworm)

Haemonchus contortus - female (barber pole worm - ruminants)

Haemonchus contortus (barber pole worm - ruminants)

Trichuris trichiura (whipworm)



A: Female


B: Male

Anterior end of ascaris worm



Should have 3 lips

Dirofilaria immitis (dog heartworm)

Nanophyetus salmonica (salmon poisoning fluke of dogs)



Notice shape and location of the oral sucker

Echinococcus granulosus (tapeworm)



Note the relatively small size and few proglottids

Haemonchus contortus copulatory bursa (barber pole worm - ruminants)

Ascaris worms (roundworms)

Anterior end of ascaris worm



Should have 3 lips

Whipworms

Trichinella encysted in nurse cell

Trichinella female filled with larvae

Paragonimus (lung fluke)

Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

Diphylidium caninum (dog/flea tape worm)

Diphylidium caninum (dog/flea tape worm)

Ancylostoma caninum anterior end (dog hookworm)

Ancylostoma caninum (dog hookworm)



A: Female


B: Male (copulatory bursa)

Ancylostoma caninum (dog hookworm)

Trichinella spiralis (nematode)

Trichinella spiralis (nematode)

Fasciola hepatica (liver fluke of sheep)

Fasciola hepatica (liver fluke of sheep)

Fasciola hepatica (liver fluke of sheep)

Fascioloides magna (deer liver fluke)

Fascioloides magna (deer liver fluke)

Paramphistomes (fluke of ruminants)

Paramphistomes (fluke of ruminants)

Diphylidium caninum (dog/flea tape worm)

Diphylidium caninum (dog/flea tape worm)

Arrange the following ascaris worms by size:



Parascaris


Ascarids


Toxocara

Ascarids (largest)


Parascaris


Toxocara (smallest)

Haemonchus contortus - female (barber pole worm - ruminants)

Haemonchus contortus anterior end (barber pole worm - ruminants)

Haemonchus contortus anterior end (barber pole worm - ruminants)

Eimeria oocysts

Eimeria oocysts

Eimeria oocysts

Eimeria oocysts

Trypanosoma cyst

Trypanosoma cyst

Trypanosoma cruzi

Trypanosoma gambiense

Trichomonas trophozoite



1 nuclei, notice the axostyle

Sarcocystis cyst

Toxoplasma gondii oocyst

Toxoplasma gondii oocysts


Left unsporulated, right sporulated

Eimeria mac (camelids)



Looks like avocados

Eimeria mac (camelids)



Looks like avocados

Isospora

Isospora

Cryptosporidium

Cryptosporidium oocyst



Sporulated, very small, no sporocyst

Cryptosporidium

Babesia

Babesia

Giardia trophozoite

Giardia trophozoite

Giardia oocyst

Giardia oocyst

American screw worm

American screw worm

Sheep nasal bot (oestrus ovis)

Horse stomach bot larvae (gasterophilus intestinalis)

Horse stomach bot larvae (gasterophilus intestinalis)

Cuterebra

Cuterebra

Ixodes pacificus (western brown legged tick)

Ixodes pacificus (western brown legged tick)

Brown dog tick



Darth Vader head

Brown dog tick

Ked

Ked

Lice

Soft tick (Argasidae)



Notice the tiny scutum

Spinose ear tick (otobius megnini)

Spinose ear tick (otobius megnini)

Lice



Left: Sucking lice (anoplura)


Right: Biting lice (mallophaga)

Lice


Left: Sucking lice (anoplura)


Right: Biting lice (mallophaga)

Centocephalides flea (cat/dog)

Centocephalides flea (cat/dog)

Echidnophaga gallinacea (hen/sticktight flea)

Echidnophaga gallinacea (hen/sticktight flea)

Demodex folliculorum (mite)

Demodex folliculorum (mite)

Sarcoptes scabei (mite)

Sarcoptes scabei (mite)

Psoroptes (mite)

Psoroptes (mite)

Dermacentor andersoni (Rocky Mountain wood tick)

Dermacentor andersoni (Rocky Mountain wood tick)

Dermacentor andersoni (Rocky Mountain wood tick)

Which parasite eggs are not easily detected via McMasters?

Certain camelid ova/oocysts & larger ova (ex: Nematodirus spp.), better to use sugar centrifugation technique

Basic steps of the sugar centrifugation technique

1. Weigh out 2 grams of feces and add to 98 ml of water - refrigerate for a few+ hours



2. Mix and then decant 10ml into centrifuge tube



3. Centrifuge for 5 minutes



4. Decant, add sugar solution, centrifuge for 5 minutes



5. Fill with sugar solution until meniscus forms add coverslip, wait for 1 hour



6. Place coverslip on slide and examine

How to quantify sugar centrifugation fecal technique results

Count the oocysts at 10x objective. Multiply the number of each type of ova/oocysts by 5 to determine EPG.



Multiply by a factor of 10 if only 1 gram of feces was used.

In what instance is the McMasters procedure superior to sugar centrifugation?

For detection of nematode strongyle eggs



Also detects roundworm eggs, tapeworm eggs, and coccidian oocysts

Basic steps of McMasters procedure

1. Get 4 grams of feces and mix with 26ml of NaCl



2. Pour suspension through gauze to remove large particles



3. Use pipette to fill both chambers of the counting slide



4. Examine under low power (10x)

How to quantify McMasters procedure results

Count both grids on the 2 chamber slide, multiply the number of eggs of each type by 25 to get EPG.