Parasitology Lab Final (Osu Cvm)

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Fasciola hepatica (liver fluke of sheep)

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Fasciola hepatica (liver fluke of sheep)

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Diphyllobothrium latum (broad fish tapeworm

Look very similar to fluke eggs, but less golden in color)

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Diphyllobothrium latum (broad fish tapeworm

Look very similar to fluke eggs, but less golden in color)

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Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores

Look at them larval hooks!)

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Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

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This is typical of which parasite(s)?

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Taenia or Echinococcus

(Notice the thick wall and larval hooks.)

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Echinococcus granulosus (tapeworm)

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Echinococcus granulosus (tapeworm)

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Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

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Diphylidium caninum (dog/flea tape worm)

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Diphylidium caninum (dog/flea tape worm)

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Fasciola hepatica (liver fluke of sheep)

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Moniezia (tape worm of sheep & other ruminants)

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Moniezia (tape worm of sheep & other ruminants)

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Baylisascaris procyonis (racoon roundworm)

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Toxocara canis (dog roundworm)

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Trichuris trichiura (human whipworm)

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Trichuris trichiura (human whipworm)

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General hookworm egg

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A: Nematodirus spp.

B: General strongyle egg

(Nematodirus is HUGE. That's why we can differentiate it from the other strongly eggs roundworms).

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Nematodirus spp. (roundworm of sheep)

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Strongyle eggs (roundworms)

(Differentiation of different roundworm species is difficult and we won't be expected to do so EXCEPT for Nematodirus which is easy to recognize because it is huge )(clearly visible on 10x).

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Ascaris (roundworm)

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Ascaris (roundworm)

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Ancylostoma caninum (dog hookworm)

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Ancylostoma caninum (dog hookworm)

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Ancylostoma caninum (dog hookworm)

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Parascaris (roundworm)

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Haemonchus contortus (female barber pole worm - ruminants)

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Haemonchus contortus (barber pole worm - ruminants)

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Trichuris trichiura (whipworm)

(A: Female

B: Male)

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Anterior end of ascaris worm

(Should have 3 lips)

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Dirofilaria immitis (dog heartworm)

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Nanophyetus salmonica (salmon poisoning fluke of dogs)

(Notice shape and location of the oral sucker)

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Echinococcus granulosus (tapeworm)

(Note the relatively small size and few proglottids)

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Haemonchus contortus copulatory bursa (barber pole worm - ruminants)

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Ascaris (roundworms)

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Anterior end of ascaris worm (Should have 3 lips)

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Trichuris (whipworms)

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Trichinella in nurse cell

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Trichinella female filled with larvae

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Paragonimus (lung fluke)

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Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

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Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

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Taenia pisiformis (tapeworm of rabbits, rodents, & carnivores)

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Diphylidium caninum (dog/flea tape worm)

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Diphylidium caninum (dog/flea tape worm)

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Ancylostoma caninum (anterior end dog hookworm)

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Ancylostoma caninum (dog hookworm)

(A: Female

B: Male (copulatory bursa))

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Ancylostoma caninum (dog hookworm)

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Trichinella spiralis (nematode)

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Trichinella spiralis (nematode)

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Fasciola hepatica (liver fluke of sheep)

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Fasciola hepatica (liver fluke of sheep)

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Fasciola hepatica (liver fluke of sheep)

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Fascioloides magna (deer liver fluke)

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Fascioloides magna (deer liver fluke)

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Paramphistomes (fluke of ruminants)

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Paramphistomes (fluke of ruminants)

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Diphylidium caninum (dog/flea tape worm)

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Diphylidium caninum (dog/flea tape worm)

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Haemonchus contortus (female (barber pole worm - ruminants))

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Haemonchus contortus (anterior end (barber pole worm - ruminants))

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Haemonchus contortus (anterior end (barber pole worm - ruminants))

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Eimeria oocysts

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Eimeria oocysts

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Eimeria oocysts

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Eimeria oocysts

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Trypanosoma cyst

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Trypanosoma cyst

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Trypanosoma cruzi

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Trypanosoma gambiense

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Trichomonas trophozoite

1 nuclei, notice the axostyle

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Sarcocystis cyst

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Toxoplasma gondii oocyst

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Toxoplasma gondii oocysts

(Left unsporulated, right sporulated)

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Eimeria mac (camelids)

(Looks like avocados)

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Eimeria mac (camelids)

(Looks like avocados)

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Isospora

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Isospora

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Cryptosporidium

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Cryptosporidium oocyst

(Sporulated, very small, no sporocyst)

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Cryptosporidium

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Babesia

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Babesia

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Giardia trophozoite

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Giardia trophozoite

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Giardia cyst

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Giardia cyst

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American screw worm

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American screw worm

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Sheep nasal bot (oestrus ovis)

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Horse stomach bot larvae (gasterophilus intestinalis)

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Horse stomach bot larvae (gasterophilus intestinalis)

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Cuterebra

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Cuterebra

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Ixodes pacificus (western brown legged tick)

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Ixodes pacificus (western brown legged tick)

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Brown dog tick

(Darth Vader head)

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Brown dog tick

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Ked

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Ked

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Lice

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Soft tick (Argasidae)

(Notice the tiny scutum)

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Spinose ear tick (otobius megnini)

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Spinose ear tick (otobius megnini)

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Lice

Left: Sucking lice (anoplura)

Right: Biting lice (mallophaga)

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LiceLeft: Sucking lice (anoplura)Right: Biting lice (mallophaga)

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Ctenocephalides flea (cat/dog)

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Ctenocephalides flea (cat/dog)

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Echidnophaga gallinacea (hen/sticktight flea)

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Echidnophaga gallinacea (hen/sticktight flea)

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Demodex (mite)

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Demodex (mite)

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Sarcoptes scabei (mite)

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Sarcoptes scabei (mite)

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Psoroptes (mite)

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Psoroptes (mite)

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Dermacentor andersoni (Rocky Mountain wood tick)

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Dermacentor andersoni (Rocky Mountain wood tick)

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Dermacentor andersoni (Rocky Mountain wood tick)

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Basic steps of the sugar centrifugation technique

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1. Weigh out 2 grams of feces and add to 98 ml of water - refrigerate for a few+ hours

2. Mix and then decant 10ml into centrifuge tube

3. Centrifuge for 5 minutes

4. Decant, add sugar solution, centrifuge for 5 minutes

5. Fill with sugar solution until meniscus forms add coverslip, wait for 1 hour

6. Place coverslip on slide and examine

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How to quantify sugar centrifugation fecal technique results

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Count the oocysts at 10x objective. Multiply the number of each type of ova/oocysts by 5 to determine EPG.

Multiply by a factor of 10 if only 1 gram of feces was used.

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In what instance is the McMasters procedure superior to sugar centrifugation?

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For detection of nematode strongyle eggs

Also detects roundworm eggs, tapeworm eggs, and coccidian oocysts

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Basic steps of McMasters procedure

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1. Get 4 grams of feces and mix with 26ml of NaCl

2. Pour suspension through gauze to remove large particles

3. Use pipette to fill both chambers of the counting slide

4. Examine under low power (10x)

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How to quantify McMasters procedure results

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Count both grids on the 2 chamber slide, multiply the number of eggs of each type by 25 to get EPG.