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102 Cards in this Set

  • Front
  • Back

Pathogenic flagellates

G. lamblia, D. fragilis, T. vaginalis

Only flagellate to inhabit small intestine

G. lamblia

Flagellate cysts that are uninuclated

Chilomastix mesnili, Retortamonas intestinalis

Characteristic shape of C. mesnili cyst

lemon or nipple-shaped

lemon or nipple-shaped

G. lamblia cyst features

with axoneme, parabasal bodies, and remnants of flagella

with axoneme, parabasal bodies, and remnants of flagella

C. mesnili cyst other features

Curved fibril alongside cytostome referred to as Sheperd's crook

Curved fibril alongside cytostome referred to as Sheperd's crook

Enteromonas hominis cyst other feature

Bipolar arrangement of nucleus, resembles Endolimax nana cyst

Bipolar arrangement of nucleus, resembles Endolimax nana cyst

Retortamonas intestinalis cyst other features

Cystostome extends above nucleus with bird's beak appearance and resembles C. mesnili cyst

Cystostome extends above nucleus with bird's beak appearance and resembles C. mesnili cyst

Motility of G. lamblia trophozoite

Falling leaf-like

Number of nuclei for G. lamblia cyst

4

Number of nuclei for E. hominis

2 or 4

Flagellates with 2 nuclei in their trophozoite form

G. lamblia and D. fragilis (80%)

Number of flagella for G. lamblia trophozoite

8

Number of flagella for C. mesnili trophozoite

4 (3 anterior and 1 cytostome)

Number of flagella for Enteromonas hominis trophozoite

4 (3 anterior and 1 posterior_

Number of flagella for Retortamonas intestinalis trophozoite

2 (1 anterior and 1 posterior)

Number of Dientamoeba fragilis trophozoite flagella

None

Number of P. hominis trophozoite flagella

4 (3 posterior and 1 in undulating membrane)

Characteristic appearance of G. lamblia trophozoite

Old man w/ glasses appearance; prominent sucking-disc and axostyle; bilaterally symmetrical

Old man w/ glasses appearance; prominent sucking-disc and axostyle; bilaterally symmetrical

Characteristic motility of C. mesnili

Cork-screw or rotary

Characteristic appearance of C. mesnili trophozoite

Prominent cytostome with spiral croove

Prominent cytostome with spiral croove

Enteromonas hominis characteristic appearance

One side flattened
One side flattened

Flagellates with jerky movement

E. hominis, and R. intestinalis

D. fragilis trophozoite characteristic feature

Multiple hyaline, leaf-like pseudopodia; nucleus has fragmented tetrakaryosome

Multiple hyaline, leaf-like pseudopodia; nucleus has fragmented tetrakaryosome

Pentatrichomonas hominis trophozoite characteristic appearance

Long undulating membrane; axostyle; and fine, scattered granules

Long undulating membrane; axostyle; and fine, scattered granules

R. intestinalis trophozoite distinguishing feature

Cytostome extend 1/2 length of the body

Cytostome extend 1/2 length of the body

General characteristics of Trichomonads

exist only in trophozoite forms, axostyle extends beyond their bodies, and equipped with anterior tuft of flagella and undulating membrane

T. tenax axostyle and undulating membrane characteristics

Thick axostyle with undulating membrane almost as long as costa

Thick axostyle with undulating membrane almost as long as costa

P. hominis axostyle and undulating membrane characteristics

Semi-rigid axosytyle with undulating membrane as long as costa

Semi-rigid axosytyle with undulating membrane as long as costa

T. vaginalis axostyle and undulating membrane characteristics

Axostyle is split into fibrils and Undulating membrane <2/3 of costa

Axostyle is split into fibrils and Undulating membrane <2/3 of costa

T. vaginalis cytoplasmic inclusions

Large amount of siderophil granules

Giardia lamblia disease caused

lambliasis, or traveler's diarrhea and has been associated with Gay bowel syndrome

Flagellate that also causes trouble with children in daycare

G. lamblia

Dientamoeba fragilis disease caused

intermittend diarrhea

T. vaginalis disease caused

Non-specific vaginitis, urethritis, or prostatitis

T. vaginalis vaginitis signs and symptoms

foul-smelling yellowish/greenish vaginal discharge accompanied by intense itchiness in vaginal area

Present forms in Leishmania

Amastigote and Promastigote

Amastigote and Promastigote

Present forms in T. brucei complex

Epimastigote and Trypomastigote

Epimastigote and Trypomastigote

Present forms in T. cruzi

Amastigote, Promastigote, Epimastigote, Trypomastigote

Amastigote, Promastigote, Epimastigote, Trypomastigote

Diagnostic stage in man (intracellular) for Leishmania spp.

Amastigote

Diagnostic stage in man (extracellular)

Trypomastigote

"Habitat" of amastigotes

inside endothelial cells and phagocytes because they cannot be degraded

Stage of Leishmania that occurs in sandfly vector and is recovered from clulture

Promastigote

Infective stage form in Leishmaniosis

Promastigote

Stage in hemoflagellate life cycle that appears in tse tse fly in T. brucei complex

Epimastigote

Diagnostic stage forms in T. brucei complex

Trypomastigote

Infective stage for T. brucei complex

Metacystic trypomastigote

Shape of trypomastigote

C, S, or U-shaped

Forms present in man in T. cruzi infections

Amastigote, promastigote, epimastigote, trypomastigote

Stages in T. cruzi infection present in bugs

Promastigote, epimastigote, and Metacystic trypomastigote

Stages in T. cruzi infection present in man

Amastigote to Trypomastigote

Leishmania spp. with Phlebotomos papataci sandfly vector

L. tropica, and L. donovani

L. brazilliensis sandfly vector

Phlebotomos intermedius

L. tropica habitat

Skin

L. braziliensis habitat

skin and mucus membranes

L. donovani habitat

visceral organs (liver, spleen, and lymph nodes)

MOT of Leishmania spp.

Skin inoculation of promastigote by sandfly vector

Cutaneous leishmaniasis

Local or metastatic skin lesions with elevated margins, which are painless but leave ugly scar on healing

Muco-cutaneous leishmaniasis description

Appear like cutaneous leishmaniasis but becomes a leprosy-like lesion called tapir nose

Visceral leishmaniasis description

fever, organomegaly, and anemia

Two kinds of cutaneous leishmaniasis

old world and new world

Old world cutaneous leishmaniasis causative agents and characteristic lesion

L. tropica and L. major causes "Oriental Sore"

New world cutaneous leishmaniasis causative agent and characteristic lesion

L. mexicana causes "Chiclero ulcer"

Visceral Leishmaniasis aka

Kala-Azar fever

Kala-Azar meaning

means darkening of skin

Causative agent of Muco-cutaneous Leishmaniasis

L. braziliensis

Trypanosoma gambiense vector

Glossina palpalis, G. tachinoides

T. rhodensiense vector

Glossina morsitans, G. swynnertoni

Brucei complex

T. gambiense and T. rhodesiense

T. cruzi vector

Reduviid, Triatoma, Panstrongylus spp.

Aka assassin bugs

Reduviid

Habitat of Brucei complex during febrile stage

Blood, Lymph

T. cruzi amastigote habitat

Endothelial and muscle cells

Habitat of Brucei complex during sleeping stage

CSF

T. cruzi trypomastigote habitat in febrile stage

Blood

T. cruzi trypomastigote habitat in sleeping stage

CSF

MOT of Brucei complex

Skin inoculation of metatrypomastigote by tse tse fly

MOT of T. cruzi

contamination of bite site and conjunctiva by bug feces

Old world or African Trypanosomiasis

Gambian (chronic) and Rhodesian (acute)

Gambian aka

West African trypanosomiasis

Rhodesian aka

East african tryponasomiasis

New World or American Trypanosomiasis aka

Chaga's Disease

Clinical manifestations of Old world Trypanosomiasis

Trypanosome chancre, Invasion of blood, Invasion of lymph nodes, Invasion of CNS

The Winterbottom sign is seen in?

Trypanosomiasis invasion of lymph nodes especially in the cervical lymph nodes

Kerandel sign is seen in?

Sleeping stage or Invasion of CNS of old world trypanosomes

2 important signs for acute New World trypanosomiasis

Romanas and Chagoma

Romanas sign definition

unilateral conjunctivitis or orbital edema

Chagoma sign definition

primary lesions that appear at site of inoculation few hours after bite

Chronic Chaga's disease signs and symptoms

Organomegaly of the heart, colon, and esophagus

Chronic Chaga's disease time frame

last for 20 years or more

Chaga's disease different types

acute, chronic, and congenital

Largest intestinal protozoan

Balantidium coli

Balantidium coli trophozoites also secretes?

Hyaluronidase

Shape of ulcer for B. coli

Wide neck with rounded base

Habitat of B. coli

Large intestine

Size of B. coli cyst

45 x 64 um

Balantidium coli cyst appearance

Has a kidney or bean-shaped macronucleus, spherical micronucleus, contractile vacuoles, and retracted cilia

Has a kidney or bean-shaped macronucleus, spherical micronucleus, contractile vacuoles, and retracted cilia

Purpose of B. coli macronucleus

for vegetations

Purpose of B. coli micronucleus

Reproduction

B. coli trophozoite size

50x70um

Morphology of B. coli trophozoite

Equipped with cell mouth (cytostome), and cell anus (cytopyge), provided with 2 contractile vacuoles, and with micronucleus at concavity of macronucleus

Equipped with cell mouth (cytostome), and cell anus (cytopyge), provided with 2 contractile vacuoles, and with micronucleus at concavity of macronucleus

B. coli characteristic movement

directional tumbling