Parasitolgy Slides

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Trophozoites of E. histolytica in a direct wet mount stained with iodine.

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Entamoeba histalytica - see flask shaped ulcer (boot shape) through the lamina propria

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Cysts of E. histolytica/E. dispar in wet mount stained with iodine.
Notice: chromatoid body with blunt, rounded ends.

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Cysts of E. Histolytica/ E. dispar stained with trichrome.
Notice: two to three nuclei (the black arrows) and the cysts contain chromatid bodies with typically blunted ends (red arrow)

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G. intestinalis cysts in a wet mount stained with iodine

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G. interstinalis trophozoite in a wet mount stained with iodine
(can see flagellates)

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Cryptosporidium parvum oocysts stained with modified acid-fast

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Cryptosporidium parvum oocysts stained with modified acid-fast; sporozoites are visible inside the oocysts.

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Immature oocyst of C. belli stained with acid-fast, showing a single sporoblast

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Cyclospora cayetanensis--> modified acid fast stain
Notice: the wrinkled edges.

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Trichomonas vaginalis; pear-shaped flagellates, undulating membranes

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Ring-form trophozoites of P. vivax in a thin blood smear

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Ring-form trophozoites of P. falciparum in a thin blood smear

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Banana shaped gametocyte of Plasmodium falciparum

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Toxoplasma gondii tachyzoites, stained with Giemsa, from a smear of peritoneal fluid obtained from a lab-inoculated mouse

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Toxoplasma gondii cyst in brain tissue stained with hematoxylin and eosin (H and E)

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Toxoplasma gondii cyst in brain tissue stained with hematoxylin and eosin (H and E)

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Toxoplasma Gondii (multiple ring enhancing lesions on CT/MRI)

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Leishmania spp. amastigotes in a Giemsa-stained tissue scraping

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Trypansoma brucei ssp. in thick blood smears stained with Giemsa

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T. cruzi trypomastigote in a thin blood smear stained with Giemsa
(notice the more anterior location of the nucleus

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Encysted larvae of Trichinella sp. in pressed muscle tissue. THe coiled larvae can be seen inside the cysts

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Fertilized egg of Ascaris lumbricoides; bile-stained egg
(see oval on the outside, the thick part is the albuminis core, the circular thing is the oval)

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Ground itch at the site of penetration (more common with ancylostoma than with nectar); hookworm

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Egg of hookworm
(see multi-lobed ovum, extremely thin egg-shell)

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Enterobiasis- non-bile stained eggs; scotch tape method

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Eggs of Schistosoma hematobium (notice the spiky thing)

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Enterobiasis- non-bile stained eggs; scotch tape method

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Trichuris trichiura (human whipworm)
resembles a beer keg; see the glassy marble things (mucous plugs at both ends)

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Looking for microfilaria--> larva of the filarial worm.

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Egg of taenia
round, looks like a marble

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Taenia saginata- no hooklets- unarmed tapeworm

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Taenia solium- head has four suckers and in the middle, see drop of hair looking thing which are hooklets used by the worm to anchor to the intestinal mucosa.

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Taenia saginata- adult worm

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Trophozoite of N. fowleri in CSF, stained with H and E
cytoplasm is granular and contains many vacuoles.
Single nucleus is large and has a large, dense karyosome and lacks peripheral chromatin.

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Immature oocyst of C. belli stained with safranin, showing a single sporoblast

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Cysts of Acanthamoeba stained with H and E from brain tissue.
Notice: may see an inner wall (endocyst) that may be hexagonal, spherical, star-shaped or polygonala and contain only one nucleus with a large karyosome.

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Cysts of Acanthamoeba stained with H and E from brain tissue.
Notice: may see an inner wall (endocyst) that may be hexagonal, spherical, star-shaped or polygonala and contain only one nucleus with a large karyosome.

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Trophozoite of Acanthamoeba sp. in tissue stained with H and E.
Often produce many spine-like processes called acnthapodia; contain a large nucleus with a large, centrally-located karyosome but no peripheral chromatin, no flagellated trophozoite stage in Acanthamoeba spp.