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7 Cards in this Set
- Front
- Back
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Restriction enzymes can be used to... |
...cut out DNA fragments. |
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What are restriction enzymes? |
Restriction enzymes are enzymes that recognise specific palindromic sequences (known as recognition sequences) and cut (digest) the DNA at these places. |
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Some sections of DNA have palindromic sequences. What are these? |
Palindromic sequences are sequences that consist of antiparallel base pairs (base pairs that read the same in opposite directions). |
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Different restrictions enzymes cut... |
...at different specific recognition sequences, as the shape of the recognition sequence is complementary to an enzyme's active site. e.g. the enzyme EcoRI cuts at GAATTC, but HindIII cuts at AAGGTT. |
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What condition needs to be true to able to extract a DNA fragment from the rest of the DNA? |
There should be a recognition sequence on either side of the fragment you want. |
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Explain how the fragment is extracted. What type of reaction is this? |
The DNA sample is incubated with the specific restriction enzyme, which cuts the DNA fragment out via. a hydrolysis reaction. |
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Sometimes, the reaction leaves sticky ends - what are sticky ends and what can they be used for? |
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