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43 Cards in this Set
- Front
- Back
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TSI |
K/NC |
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what pathway is utilized? |
Entner-Douderoff |
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what are the end products? |
weak acids |
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what media can differentiate some types? |
growth on MAC/EMB |
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are they aerobic? |
yes |
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do they grow slowly? |
no, rapid growers |
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how would you set up tubes for dextrose/glucose? |
two tubes: one closed, one open |
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how long should you hold oxidative/fermentative media? |
2-5 days |
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when is the flagellar stain useful? |
when an organism has weak biochemical reactions? |
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what do water insoluble pigments do? |
color the organisms |
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what color are phenazines? |
red, maroon, yellow |
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what is pseudocel agar? |
a color enhancement agar that inhibits non-pseudomonas app |
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Tech or Flo media? |
color enhancement |
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for glucose nonfermenters, what color is the control tube in amino acid decarboxylation? |
purple glucose is not utilized |
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what does gelatin hydrolysis test for? |
gelatinase |
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what is a positive photographic film strip test for gelatin hydrolysis? |
clearing of the strip |
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what test is used to distinguish moraxella catarrhalis and acinetobacter spp from other moraxella spp? |
penicillin disk test |
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what result on gram stain will you see with other moraxella spp from the penicillin disk test? |
inner zone: long rods outer zone: coccobacilli |
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what is different about the lead acetate test and the TSI test? |
lead acetate can detect very small amounts of H2S |
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where is pseudomonas aruginosa found? |
moist environments |
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what types of patients typically get pseudomonas infections? |
hospitalized patients on a ventilator |
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name common infections from pseudomonas |
burn infections external ear infections eye infections in contact lens users |
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what type of patients have a high mortality rate from pseudomonas infection and cannot clear their lungs? |
cystic fibrosis patients |
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what causes some strains of pseudomonas to be extremely mucoid? |
alginate polysaccharide polymer |
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grain stain of pseudomonas |
slender gram negative rod |
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colony morphology |
BAP: large, spreading, grape odor, green metallic sheen MAC: NLF |
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what two pigments does pseudomonas produce? |
pyocyanin pyoverdin |
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what type of media may be added to observe pseudomonas' green color |
pseudocel agar |
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you must do______________for pseudomonas why? |
susceptibility testing widespread resistance |
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treatments of choice for pseudomonas? |
3rd generation cephalosporins Piperacillin Piperacillin/tazobactam Aminoglycosides |
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what is P. Fluorescens associated with? |
contaminated blood products |
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what test can differentiate P. fluorescens and P. putidi? |
gelatin test fluorescens is positive |
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what groups of people is acinetobacter baumanii? |
immunocompromised wounded military under stress the elderly catheter use breathing tubes |
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what are the most common and second most common non-fermenter isolated in the clinical lab? |
pseudomas aruginosa acinetobacter baumanii |
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acinetobacter baumanii gram stain |
cocci or diplococci gram variable |
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acinetobacter baumanii colonial morphology |
BAP: pink tinge |
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what sugars does acinetobacter baumanii oxidize? |
glucose lactose |
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susceptibility testing must always be done because |
high resistance rate to penicillin, ampicillin, 1st generation cephalosporins, aminoglycosides |
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what drugs is acinetobacter baumanii susceptible to? |
meropenem, amikacin and minocycline Beta lactam + aminoglycoside |
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what organism is never part of our normal flora? |
acinetobacter iwoffi |
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why should a growth control or purity plate always be used with acinetobacter iwoffi? |
it is asaccharolytic and biochemically inert |
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colonial morphology of S. maltophilia? |
BAP: yellow green pigment, ammonia like odor MAC: positive for growth |
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