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7 Cards in this Set
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- 3rd side (hint)
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NMR depends on the ----- of an atom involved. NMR requires a ---- that is capable of existing in ----- ----- under ----- conditions and therefore it is highly ----- proteins in ---- phase. |
Nucleus Sample Highly concentrated Acidic Soluble Liquid |
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What parts of the structure have relatively stronger NMR signals? |
Flexibility |
X-ray crystallography has weak signals for this region |
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Sequence specific assignments step uses which experiments? |
Tocsy total correlation sequence Nosey nuclear Overhauser effect spectroscopy |
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Tocsy is where ---- belonging to individual ---- of ---- ---- ---- are identified. Cross peaks are generated on ---- maps which represent the ---- atom of one ---- --- side chain. The ---- made via --- bonds are also observed. |
Resonances Types Amino acid residues Contour Hydrogen Amino acid Connections Chemical |
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Nosey exp observes cross peaks on contour maps that --- resonances from --- that are --- close within --- Å than those connected via --- ---. This orders ---- neighbouring amino acids by observation of --- --- ----. |
Connect Nuclei Spatially 5 Chemical bonds Sequentially Nuclear overhauser effect |
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J coupling? NOE values? |
J coupling is the measurement of of covalent bonds that separate protons. The 3 bond j values give information on torsion angles which determines dihedral angle constraints. NOE values are determined by the dipole dipole coupling between protons. All resolvable NOE values are quantified to give approximate distance constraints |
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NOE intensities are calculated with |
NOE proportional to 1/r6 |
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