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194 Cards in this Set
- Front
- Back
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Mycoplasmatales |
Order where mycoplasma and ureaplasma belong |
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Mollicutes |
mycoplasma and ureaplasma are collectively called |
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Mollicute |
Has small genome and lack genetic capacity to produce |
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Mollicute |
Smallest free living prokaryotes |
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Archole Anaeco Spiro Myco Urea astero |
Other genera of mycoplasmatales |
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Guanine Cytosine |
Mycoplasma has low _ content of DNA |
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Mycoplasma |
Genus under mycoplasmatales that has No cellwall |
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Pink or purple |
Color of stain of mycoplasma by giemsa, castaneda,dienes, m blue |
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giemsa, castaneda,dienes, m blue |
Stains of mycoplasma |
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Cytoplasmic membrane |
Composed of phospholipids, sterols, glycolipids, proteins, glycoproteins, and cholesterol for osmotic stability |
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Mycoplasma mycoides subsp. Mycoides |
Covered by capsular polysaccharidE |
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Polar pleb |
Specialized tip structure of sp of mycoplasma that has role in adherence to host cell surface |
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Cholesterol Related sterol |
Required for growth of mycoplasma |
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Serum |
Source of cholesterol |
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37 |
Optimum growth temp of Mycoplasma |
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Facultative anaerobic |
O2 req of mycoplasma |
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3-7 days |
Incubation time of mycoplasma |
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Minute Fried egg appearance |
Typical colonies of mycoplasma |
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Embryonated egg Cell culture systems |
Mycoplasma can also be propagated in these and more strains can grow.these are valuable for growth of fastidious strains |
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Negative |
Rxn of mycoplasma in catalase test |
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Peroxide Superoxide |
Cellular product of mycoplasma that are imp in disruption of host cell integrity |
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moist cool environmemt |
Mycoplasma can survive outside the host in substantial period of |
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Heat Most detergent Disinfectant |
Mycoplasma are very susceptible to |
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Tween |
Ex of detergent wherein mycoplasma is very susceptible |
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QAC iodine Phenol-based |
Ex of disinfectant wherein mycoplasma is very susceptible |
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Mycoplasma |
Cannot survive refrigeration |
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Mycoides subsp mycoides |
Type spp of mycoplasma |
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Contagious bovine pleuropneumonia |
Ds caused by m. Mycoides subsp mycoides in cattle |
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Arthritis |
Ds caused by m. Mycoides subsp mycoides in lamb |
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Small and large colony type |
2 types of m.Mycoides subsp mycoides |
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Small colony type |
Type of m. Mycoides subsp mycoides isolated mostly in cattle |
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Large colony |
Type of m. Mycoides subsp mycoides isolated mostly in goats and occasional in sheep |
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Cattle and buffalo |
m. Mycoides subsp mycoides is an obligate parasite of what animal |
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Droplet inhalation |
Transmission of m. Mycoides subsp mycoides |
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Inapparent reservoir of infection |
Animal with sublinical ds and those that recover |
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7-8 mo |
How many months does the natural ds of m. Mycoides subsp mycoides |
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Extreme resp distress |
Signs of acute ds caused by Mycoides subsp mycoides |
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Moist cough High temp Roughened coat Nasal discharge Reluctant to move |
Charac of chronic form of ds caused by Mycoides subsp mycoides |
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15- 20L |
How many L does the pleural cavity in necropsy contains? |
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Capsular galactan |
Has pathophysiological effects (Mycoides subsp mycoides) |
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Production of pulmonary edema Capillary thrombosis Collagen deposition Immuno-suppression Bacteremia Neutropenia Lymphocytosis |
Pathophsyiological effects of m. Mycoides subsp mycoides |
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M. Alkalescens |
Commonly found on respiratory and urogenital mucosal surface of cattle where it is normal commensal |
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Arthritis |
Ds caused by m. Alkalescens in calf |
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Mastitis |
Ds caused by m. Alkalescens in cow |
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M. Alkalescens |
Grows slowly and sparsely in standard mycoplasmal media |
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Arginini |
Respiratory, conjuctival, and vaginal commensal |
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Bovigenitalium |
Oppurtinistically invade mucosa of vulva, vagina and uterus causing vulvovaginitis |
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Mastitis in M. Bovigenitalium |
Charac by firm, swollen, painless quarter, reduced milk flow and yellowish secretion |
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Addition oF DNA |
M. Bovigenitalium's growth on media is markedly enhanced by _ |
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Urogenital tract |
M. Bovigenitalium is a normal commensal of |
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Bovirhinis |
Low virlunce, Resp. Commensal and causes mastitis |
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M. Bovis |
High virulence next to rank m. Mycoides subsp. Mycoides |
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Severe mastitis Arthritis Calf pneumonia Abscesses and otitis |
Ds caused by m. Bovis |
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Giemsa and methylene blue |
Stain of m. Bovis |
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Ring and amorphous clumps |
Appearance of m. Bovis giemsa or methylene blue |
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63 days at 4c 14 days at 20c |
M. Bovis survives milk in |
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Poorly husbandry Sanitation |
Predisposing factors |
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Sudden/sharp drop in milk prod Extremely swollen udders that are not painful Supramammary LN are enlarged and swollen Slightly yellow secretion, forms sediments upon standing |
Charac of mastitis in m. Bovis |
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M. Bovoculi |
Highly virulence in presence of moraxella bovis |
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Conjunctivitis Keratoconjunctivitis |
Dss caused by m. Bovoculi |
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Spring Summer |
M. Bovoculi is prevalent during what season? |
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Acute mastitis Arthritis |
Dss caused by m. Californicum |
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Acute mastitis (m. Californicum) |
Ranks 2nd in frequency of isolation from outbreaks of mycoplasmal mastitis |
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Swelling of udder Watery yellow secretion with flakes, clots, pus and sandy sediments |
Charac of mastitis caused by m. Californicum |
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Conical Smooth With distinct centers |
Colonies of m. Californicum |
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Canadense |
Less persistent than m. Bovis |
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Mastitis in cow Arthritis in calves |
Dss caused by canadense |
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Resp. Tract Prepuce |
M. Canadense is a commensal of |
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Alkalescens Arginini Canadense |
Spp of mycoplasma that hydrolyzes arginine |
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Bronchiolitis Alveolitis |
Dss caused by m. Dispar |
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Resp traCt |
M. Dispar is commensal of |
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M. Dispar |
Colonies on primary culture lack defined center typical of most mycoplasma sp |
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M. Dispar |
Has presence of extracellular capsule and they are fastidious grower |
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Conjunctivitis |
M. VerEcundum causes _ in calves |
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Prepuce |
M. Verecundum is a commensal of |
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M. Verecundum |
Lacks any outstanding biochemical features |
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Intestine and vagina |
M. Alvi is commensal of |
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Mycoides subsp. Mycoides Alkalescens Arginini Bovigenitalium Bovirhinis Bovis Bovoculi Californicum Canadense Dispar Verecundum Alvi |
Mycoplasmal spp of cattle |
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Mycoides subsp mycoides Bovis Bovoculi Californicum |
Mycobacteria sp in cattle that is highly virulence |
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Alkalescens Bovigenitalium Canadense Dispar |
Mycobacteria sp in cattle that is moderately virulence |
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Arginini Bovirhinis Verecundum Alvi |
Mycobacteria sp in cattle that is low virulence |
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Contagious agalactia Arthritis Vulvovaginitis Conjunctivitis Pneumonitis |
Dss caused by m agalactiae |
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M. Agalactiae |
Spp that dont metabolize nor hydrolyze arginine |
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Phosphatase |
M. Agalactiae produce |
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Tetrazolium |
M. Agalactiae reduce |
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Enpoparasite |
Plays role in transmission of m. Agalactiae |
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M. Capricolum |
Has lowered req of chol than other mycoplasmal sp |
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Ingestion of infected colostrums/milk for septicemia in kids Inhalation |
Transmission of m. Capricolum |
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Greenish or olive |
Colonies of m. Conjunctivae |
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Sheep Goat Chamois |
Hosts of m. Conjunctiviae |
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Keratoconjunctivitis |
Ds caused by m. Conjunctivae |
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M. Mycoides subsp capri M. Strain F38 |
Causative agents of contagious caprine pleuropneumonia |
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Oleic acid |
Enhances filament formation of M. Mycoides subsp capri |
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Minimum cholesterol |
Growth req of M. Mycoides subsp capri |
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Inhalation of drOplets |
M. Mycoides subsp capri mode of transmission |
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Hydrogen peroxide |
Produce by M. Mycoides subsp capri that inhibits activity of epithelial celia |
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6-10 days |
Incubation period of M. Mycoides subsp capri |
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100 |
Morbidity percentage of M. Mycoides subsp capri |
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70 |
Mortality percentage of M. Mycoides subsp capri |
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Cough Weakness Lying down with labored respiration Fever (40.5- 41.5) |
Signs of M. Mycoides subsp capri infection |
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Septicemia |
Observed in animals with highly acute ds in M. Mycoides subsp capri |
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M. Putrefaciens |
Produces strong putrefactive odor from broth culture |
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No clinical evidence of abnormality except a drop of milk flow and presence of inflammatory cells in secretion |
Charac of mastitis caused by m. Putrefaciens |
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Sheep |
Resistant to m. Strain F38 |
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Septicemia |
Ds caused by m. Strain F38 |
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P.haemolytica |
M. Ovipneumoniae causes chronic pneumonia in association with |
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M. Ovipneumoniae |
Morphology is poorly defined + colonies are unusual because they lack distinct central area |
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Septicemia Polyarthritis Mastitis Keratoconjunctivitis |
Ds caused by m. Mycoides subsp mycoides in sheep and goat |
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Agalactiae Capricolum Conjunctiviae Mycoides subsp capri Putrefaciens Strain F38 ovipneumoniae Arginini Mycoides subsp mycoides |
Mycoplasmal sp of sheep and goat |
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M. Flocculare |
Has typical mycoplasma morphology |
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Fried eggsl |
Its colonies donot look fried eggs |
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A26 of FF MEDIUM |
Requirement of m. Flocculare for growth |
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Enzootic pneumonia of pigs |
Ds caused by m. Hypopneumonia |
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Chronic non productive cough |
Signs of m. Hypopneumonia |
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Virus pneumonia of swine Infectious pneumonia of pigs Ferkelgrippe |
Syn of Enzootic pneumonia of pigs |
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Slow weight gain Insufficient food conversion |
M. Hypopneumonia causes severe economic hardship due to |
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Thru aerosol |
M. Hypopneumonia is transmitted thru |
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18- 14 |
Incubation period of m. Hypopneumonia |
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3-10wks |
Age of pigs usually showing signs |
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Hepatized Purplish or grayish pneuomonic areas in apical and cardiac lobes of lungs |
Lesion caused b m. Hypopneumonia |
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Upper resp. Mucosa |
M. Hyorhinis is a commensal of |
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Arthritis Pneumonia Polyserositis |
Dss caused by m. Hyorhinis |
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Polyserositis |
Charac by serofibrinous synovitis and arthritis, pericarditis, pleuritis and peritonitis |
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B. Bronchiseptica P. Multocida EPP |
M. Hyorhinis is secondary invader to |
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M. Hyosynoviae |
Affects 12-24 week old pigs |
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Nasopharynx |
M. Hyosynoviae is commensal of |
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Suppurative polyarthritis |
Ds caused by m. Hyosynoviae |
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Adult pig |
Carrier or reservoir of m. Hyosynoviae |
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Hampshire |
Breed of pig that develops more severe disease following infection than other breeds |
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Flocculare Hypopneumonia Hyorhinis Hyosynoviae |
Mycoplasmal dss of swine |
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Anitis Cloaca Columbinasale Columbinum Columborale |
Mycoplasma spp in poultry with low virulence |
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M. Anatis |
Causes sinusitis, commensal of resp tract and cloaca of duck |
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M. Cloaca |
Commensal of cloaca of turkey |
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M. Columbinasale |
Causes mild resp ds and commensal of upper resp tract of pigeon |
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M. Columbinum |
Commensal of upper resp tract of pigeon |
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CRD IST |
M. Gallisepticum is asstd with ds complex such as |
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Polar bleb |
Serves as an adhesion organ and contributes to gliding motility |
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Classical fried egg colonies |
Morphology of colony of m. Gallisepticum |
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Polar bleb Glyophorin |
Attachment involve in m. Gallisepticum |
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Glyophorin |
Glycoprotein found jn surface of erythrocyte and rich in sialic acid |
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By aerosol By contact By egg transmission |
Means of transmission of m. Gallisepticum |
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During egg formation in ovary During egg passage in oviduct |
Egg transmission of m. Gallisepticum is during |
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Polar bleb and capsular materials Ciliostatic property |
Pathogenic attributes of m..gallisepticum |
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Polar bleb and capsular material |
Mediate adhesion to tracheal and bronchial epithelium |
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Ciliostatic property |
Imp in colonization and avoidance of mucociliary escalator |
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Infectious sinusitis of turkey |
Charac by swelling of infraorbital sinuses filled with thick mucoid exudates |
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M. Iowae |
Causes embryo mortality and air sacculitis |
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M. Meleagridis |
Causes air sacculitis, perosis, arthritis, salpingitis, synovitis, skeletal abnormalities in turkey |
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Biotin |
Stimulates the growth of m. Meleagridis |
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Synovitis and air sacculitis |
Dss caused by m. Synoviae in chicken and turky |
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Air sacculitis |
Ds caused by m. Synoviae that is extensive at low ambient temp regardless of humidity |
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Broiler breed |
Breed of chicken that is more susceptible than laying breeds |
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M. Synoviae |
Similar morphologic and cultural features with m. Gallisepticum |
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Infected eggs Infected vaccines Contact of infectious aerosols |
Source of infection of m. Synoviae |
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Infected eggs |
Most frequent source of infection and transmitted vertically |
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Infected vaccines |
Lateral source of infection of m. Synoviae |
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Infectious synovitis Air sacculitis |
Clinical manifestations of m. Synoviae |
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Infectious synovitis |
Clinical manifestation of m. Synoviae that has swollen joints and tendon sheaths |
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Hocks Feet Bursae or breast |
Areas affected by infectious synovitis of m. Synoviae |
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Air sacculitis |
Clinical manifestation of m. Synoviae that has slight rales and lining of air sacs are thickened with whitish yellow exudates |
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Vulvar lesion |
Lesion of endocarditis in air sacculitis caused by m. Synoviae |
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Anemia |
Caused by hemolytic effect of hydrogen peroxide which is produced by m synoviae that have adhered to erythrocytes |
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Gallisepticum Iowae Meleagridis SyNoviae |
Mycoplasma spp that are higly virulent to poultry |
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Arginini Equirhinis Salivarium Felis Mycoides Equigenitalium |
Mycoplasma spp of horses |
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ArgininiEquirhinisSalivarium |
Mycoplasma spp of horses commonly found on upper resp mucosa and are harmless commensals |
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M. Felis |
Recovered from thoracic cavity of horse with pleuritis |
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M. Mycoides |
Found in bronchial secretions of horses with resp dss |
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M. Equigenitalium |
Detected in stallion semen |
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Glyceryl phosphorylcholine Fructose Total protein |
Presence of m. Equigenitalium is correlated with lower levels of |
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Felis Gateae |
Commonly encountered spp of mycoplasma in cats |
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M. Felis |
Spp of mycoplasma in cats that is associated with conjunctivitis and other dss |
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M. Gateae |
Normal commensal of feline oropharynx and has potential to enter bloodstream and cause polyarthritis |
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Arginini Arthritidis Feliminutum Gallisepticum Pulmonis |
Spp of mycoplasma revcovered from cat specimen |
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11 |
How many spp of mycoplasma in dogs? |
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M canis |
Pathogenic for genitourinary tracts of dogs |
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M. Cynos |
Pathogenic for resp tract of dogs |
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Hydrolyze urea |
Ability of ureplasma that distinguished them from members of mycoplasma |
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Ammonia |
Ureaplasma produces what upon hydrolysis that is toxic to host tss |
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15-60um |
Colony size of ureaplasma |
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6 |
Optimum ph of ureaplasma |
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U. Urealyticum |
Spp of ureaplasma found in humans |
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U. Diversum |
Spp of ureaplasma found in animals |
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Ruminants Horses Poultry Cats Swine Dogs |
U. Diversum is often in mucosa of these animals |
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Shape of ureaplasma and mycoplasma |
Coccoid |
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Filament formation |
Formation of ureaplasma that occur only in old culture |
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0.05 M HEPES buffer Manganese sulfate |
Added to agar medium of ureaplasma to aid in detection of ammonia |
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Dark brown |
Color of colonies of ureaplasma |
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Small colony size Optimum ph Low cell productivity in broth cultures |
Other distinguishable features of ureaplasma |
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Mastitis Vulvitis |
Ds caused by ureaplasma in ewes |
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Cuffing pneumonia |
Ds caused by ureaplasma in calves |
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Vulvovaginitis |
Ds caused by ureaplasma in heifers and cows with history of infertility |
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Calculi |
Ureaplasma contributes to formation of _ in grain fed lambs |
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Bovine ureaplasma |
Produces IgA protease which cleaves IgA1 and may aid in avoiding hosts IR on mucosal surface |
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Protease Hemolysin Nucleases |
Bovine ureaplasma produces _ |
