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144 Cards in this Set
- Front
- Back
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They resist decolorization effect of acid-alcohol after it is stained with hot carbol-fuschin |
Why is it mycobacterium are acid fast organism? |
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60% |
Percentage of lipid in cell wall of mycobacterium |
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Hydrophobicity in fluid media Slow growth and resistance to acids, disinfectants, antibodies and dessication |
High lipid of mycobacterium is responsible for |
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High mol wt mycolic acid Trehalose 6, 6' dimycolate (cord factor) |
Cellwall of mycobacterium composed of waxes and glycolipid containing : |
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Trehalose 6,6' dimycolate or cord factor |
Immobilizes N, act as adjuvants, evoke granulomatous responses and cause mitochondrial disruption |
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Bovis Avium Tuberculosis Paratuberculosis |
Classical spp of mycobacterium |
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Mycobacteriosis |
Ds caused by m. Avium in fowl |
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Porcine |
Other host of m. Avium |
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Man |
Primary host of m. Tuberculosis |
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Bovine |
Primary host of m. paratuberculosis |
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Jhone's disease |
Laymans term for ds caused by m. Paratuberculosis |
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Runyon groups |
Composed of spp. Of mycobacterium that occur widely in feces and nature |
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Colony pigmentation Growth rate |
Basis of classification of runyon groups |
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Photochromogenic strains Schotochromogenic strains Notochromogenic Rapid growers |
4 classification of runyon groups |
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Ulcerans Marinum Kansasii |
Spp under photochromogenic strains |
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Yellow |
Color of pigment in light of photochromogenic strains |
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Ulcerans Marinum |
Producers of skin lesion |
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Kansasii |
Causes pulmonary ds in humans |
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Orange-yellow pigment |
Color of pigment in dark or light |
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Scrofulaceum |
Sp under scotochromogenic strains |
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Notochromogenic strains |
Strain that Dont produce pigment and resistant to isoniazid |
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Avium |
Sp under notochromogenic strains |
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Rapid growers |
Strains that Mature in less than 1 wk @ 25-37C |
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Fortuitum Phlei Smegmatis |
Spp that are rapid growers |
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Leprae Lepraemurium |
Spp of mycobacterium that are obligate parasites |
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Avium Bovis Fortuitum Kansasii Tuberculosis |
Spp of mycobacterium that are facultative intracellular parasites |
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Phlei |
Ex of saprophytes spp of mycobacterium |
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Bovis Avium Paratuberculosis Farcinogenes |
Most imp. Ds-producing spp of mycobacterium in domestic animals |
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Johne's ds |
Debilitating ds of cattle charac by chronic or intermittent diarrhea |
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M. Farcinogens |
Found in tropical countries and cause of bovine farcy |
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Fortuitum Phlei |
Spp of mycobacterium found in cutaneous lesion of animals or in udder |
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Lepraemurium |
Sp of mycobacterium causes feline leprosy |
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Feline leprosy |
Cutaneous granulomatous type of ds |
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Dogs Goats Sheep |
Animals that are relatively resistant to m. Bovis |
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Birds |
M. Bovis does not cause ds in _ |
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Short, relatively plump, gram positiVe rods |
Appearance of m. Bovis in tissue |
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Larger, slender, and beaded |
Appearance of m. Bovis in culture |
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Red |
Color of stain of m. Bovis in ziehl-neelsen staining technique |
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Glycerol |
Inhibit the growth of m. Bovis |
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Dorset Stonebrinks Lowenstein- jensen's medium |
Media used in m. Bovis |
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Lowenstein's jensen's medium |
Complex organic media for cultivation of mycobacterium at 33-39c prod dry and crumby colonies |
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Pyrazinamide |
M. Bovis is resistant to |
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Dessication, acids and alkalis |
M. Bovis is resistant to_ |
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Pasteurization and sunlight |
M. Bovis is destroyed by |
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Malachite green |
Dye that inhibits contaminants of m. Bovis |
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Phenolic disinfectants |
Most effective disenfectant of m. Bovis |
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Waxes d Mycosides Cordfactor |
Lipid in cell wall of m. Bovis |
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N-acetylmerramyl dipeptide |
Potent enhancer of humoral and cell mediated IR |
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Mycosides |
Cell wall component that is Glycolipid containing high mol wt fatty acid |
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Tuberculoprotein/ tuberculin |
Protein or polypeptide released into the medium that is active in cutaneous delayed hypersensitivity rxn |
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Tuberculoprotein/ tuberculin |
Powerful aid in detection of infected animals and useful in diagnostic reagent |
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Mycobactin |
Cell wall amines involved in iron acquisition |
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Aerogenous Milkborne Congenital Sexually transmitted |
Routes of transmission of m. Bovis |
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Aerogenous |
Principal route of m. Bovis |
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Prepucial carriage |
Sexual transmission of m. Bovis is tru |
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Cord factor |
Stimulate granulomatous responses |
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Mycosides Phospholipids Sulfolipids |
Protects tubercle bacilli from phagocytic killing |
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Tuberculoproteins |
Stimulate cell mediated responses and central feature of tb |
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Sulfatides |
M. Bovis produce this for accumulation of lysosome |
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Lymphokines |
Released by T-lymphocyte to 8nduce inflammatory response |
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Regional LN |
Filters organisms that escape activated macrophages |
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Tubercles |
Lesion caused by m. Bovis |
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Aggregation of macrophage Lymphocytes Other leukocytes |
Tubercule consist of : |
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Tubercules |
Transluscent, pearly structures similar to small grains of tapioca |
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Yellowish white opaqueness |
Color of necrosis that begins in center as tubercle grow |
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Langhans type |
Giant cells formed from macrophage by continued growth and multiplication of nuclei |
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Primary sites Regional LN sites of secondary metastasis |
Site of lesion of m. Bovis |
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Bronchial LN Lung lesion |
Sites of lesion of m. Bovis in cattle |
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Mediastinal LN submaxillary LN retropharyngeal LN |
Specific sites of lesion of m. Bovis in Bronchial LN that exhibits visible lesion than lungs |
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Anterior lobe |
Site of lung lesion of m. Bovis |
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Gross necropsy findings Smear exam by Ziehl-neelsen proc. Bacteriological cultural isolation Inoculation of guinea pig, rabbit and chicken Tuberculin test |
Diagnosis of m.bovis |
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Skin of caudal fold Sides of neck Lip of vulva |
Sites of tuberculin test in m. Bovis |
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M. Avium |
Produce tb in birds |
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Group 3 |
Group of m. Avium in runyons classification |
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Dome-shaped colonies |
Colobies of m. Avium in droset's egg or solid media |
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Cream colored |
CoLor of m. Avium in droset's egg |
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Considerable mucoid sediments |
Growth in liquid culture of m. Avium |
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25- 45 |
Growth temp of m. Avium |
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Glycerol |
Growth enhancer of m. Avium |
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Engonic |
M. Avium in terms of glycerol |
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Dysgonic |
M. Bovis in terms of glycerol |
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Birds |
Natural host of m. Avium |
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Geese and ducks |
Infcted less often by m. Avium than other domestic birds |
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Horses |
Other animal infected by m. Avium aside from bird |
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Ingestion Egg transmission |
Routes of entry of m. Avium |
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Loss of weight Weakness Listlessness Eventual death Lameness and wing drooping due to bone and joint lesions |
Manifestations of m. Avium |
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Intestinal tract Liver Spleen |
Other lesion of m. Avium |
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Ziehl- neelsen method |
Methot of staining for m. Avium |
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Cervical LN |
Lesion of m. Avium in children |
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M. Tuberculosis |
Most common cause of tuberculosis in humans and primates |
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M. Tuberculosis |
Type sp of genus mycobacterium |
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Dogs and members of psittacidae (parrot) |
Animals that are Most susceptible in m. Tuberculosis |
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Pleurisy or peritonitis |
M. Tuberculosis develop _ when seen in fluid |
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Rabbits |
M. Tuberculosis is avirulent in |
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Jhone's bacillus M. Enteritidis M. Johnei |
Syns of m. Paratuberculosis |
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Jhone's ds Paratuberculosis Chronic bacterial enteritis Chronic hypertrophic enteritis |
Dss caused by m. Paratuberculosis |
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Mycobactin |
M. Paratuberculosis has strict req for this iron chelating agent |
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Clumps |
Appearance of m. Paratuberculosis in tissues and feces |
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Mycobactin Exochelin |
Iron chelating compounds |
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J |
Better source of mycobactin than P |
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M. Paratuberculosis |
Source of j |
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M. Phlei |
Source of P |
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Hexadecylpyridinium chloride 5% oxalic |
Remove contaminating organisms from specimens of intestines and fecal material |
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Herrold's egg-yolk medium |
Medium of choice of m. Paratuberculosis |
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Exochelin |
Prtotein that remove ferric iron from feratin |
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Acids Alkalines |
M. Paratb is resistant to _ |
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Moderate heat 5% formalin or 5% lysol |
M. Paratb are rapidly destroyed by |
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Jersey and shorthorn |
More susceptible in m. Paratuberculosis than other breeds |
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Cattle raised in alkaline soil |
Less susceptible in m. Paratb |
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Pig |
Can serve as intermediate host for m. Paratb |
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Suckling calves |
Has greater risk of m. Paratb infections |
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5% |
Only _ develops obvious clinical ds of m. Paratb |
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95% |
Percentage of subclinical infection that causes greater economic lossses |
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Ingestion |
Mode of transmission of m. Paratb |
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Hypoalbuminemia |
Condition due to loss of protein and failure of absorption of nutrients |
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Sheep and goat |
These animals does not show signs of diarrhea, instead, chronic wasting |
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False positive results |
Due to cross reactivity to related bacteria or due to prev exposure to organisms |
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FalsE negative results |
Due to tolerance |
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Serologic Allergic Culture and isolation Microscopic examination of fecal or mucosal smear |
Diagnostic tests for m. Paratb |
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ELISA Complement fixation Agar gel immunodiffusion crossed immunoelectrophoresis Radio immunoassay Immunoperoxidase |
Serologic tests used in m. Paratb |
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Complement fixation Agar-gel immunodiffusion |
Most widely used serologic test in m. Paratb |
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Crossed immunoelectrophoresis |
More sensiteve but more difficult serologic test of m. Paratb |
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Intradermal inoculation test of jhonin Lymphocyte transformation test Macrophage migration Macrophage inhibition test |
Allergic tests in m. Paratb |
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Feces Scraping of rectal mucosa Mesenteric LN biopsy Iliocecal LN biopsy |
Specimens used in m. Paratb |
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Clumps |
Appearance of m. Paratb in mucosal smear |
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Small shred of mucus |
Appearance of m. Paratb in fecal samples |
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Bovine farcy |
A ds of cattle in tropical countries caused by m. Farcinogenes |
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M. Farcinogenes |
Only sp of mycobacterium that exhibits branching |
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37 c in 15-20 days |
Growth of m. Farcinogenes accurs at_ in _ |
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Petragnani medium Modified sauton's medium |
Media used in m. Farcinogenes |
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Mycolates Ketomycolates Methoxymycolates |
Cell wall components of m. Farcinogenes |
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Smear Inoculation of guinea pig |
Diagnosis of m. Farcinogenes |
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Gram stain Ziehl-neelsen |
Stain of m farcinogenes |
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Canaries Parrots |
Animals affected by m. Genavense |
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Psittacines |
Resistant to m. Avium but sensitive to m. Tb |
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Kansasii Intracellulare Marinum Scrophulaceum Xenopi Phlei Fortuitum Gordonae |
Ex of saprophytic acid fast bacilli |
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Fortuitum Phlei |
Forms cutaneous granulomatous in cats |
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Intracellulare |
Frequent cause of lymphadenitis in pigs |
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M. Lepraemurium |
First observed in rats in lesions that resembles human leprosy |
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M. Lepraemurium |
Causative agent of feline leprosy |
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Feline leprosy |
Granulomatous disease of skin of head and limbs of cats |
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Ulcerative lympangitis (skin Tb) in cattle |
Lesion occur in lower part of legs |
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Mycobacterial ulcerative dermatitis |
Asstd w/ opportunistic mycobacteria |
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Bovine mycobacterial ulcerative lymphangitis |
Noduloulcerative skin lesions in cattle and Resembles tubercle |
