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32 Cards in this Set
- Front
- Back
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Aerobic, non-motile; slow grower and require whole egg for growth |
Mycobacterium |
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Difficult to stain because? |
Due to hydroxy methoxy acid/mycolic acid |
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Intestinal TB in man |
M. bovis |
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Tb in man in africa |
M.africanum |
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4 runyon's classification |
Photochromogens Scotochromogens Non-photochromogens Rapid growers |
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Hansen's disease |
M. leprae |
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Mice food pads or foot pads of armadillo |
M. Leprae |
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Cigar packet arrangement |
M. Leprae |
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Steps in mycobacterium detection |
1st: classified as sterile or non-sterile 2nd: if sterile (centrifuge) if non sterile (do digestion and decontamination) 3rd: (sediment) smear, preparation & culture 4th: screening test which is the acid fast stainining |
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Carried out to remove normal flora and other contaminating organisms |
Decontamination |
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Dissolve disulfide bond in mucus which could trap MTB |
Digestion |
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Gold standarf fo digestion and decontamination |
NALC (N-acetyl-L cysteine and NaOH) or dithiotreitol or sputolysin and NaOh |
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Add oxalic acid why? |
Likely contain pseudominas |
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Prolong shelf life of sputum? Up to what days |
1% cetylpyridium chloride, 8 days |
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Useful in the identification of M. Kansasii |
Tween 80 hydrolysis test (rapid) |
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For the identification of M. avium |
Tellurite reduction test (+) black mettalic tellurium |
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For identification of rapid growers and M. Triviale |
Salt tolerance test 5% NaCL |
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For the differentiate M. bovis and m. Tuberculosis |
M. bovid is susceptible T2H/ TCH susceptibility thiopene-2-carboxylic hydrazide |
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Koch bacillus |
M. tuberculosis |
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Virulence factor of M. Tuberculosis |
1. Cord factor 2. Sulfatides |
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M. tuberculosis |
Much granules |
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Vaccine for M. tuberculosis |
BCG/ Bacillus Calmette Guerin |
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Skin test for M. tuberculosis |
Mantoux test 1. PPD 2. Von pirquet 3. DOTS |
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Unpasteurized milk (tuberculosis) |
M. Bovis |
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Water droplet colonies |
M. bovis |
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Hansen's bacillus |
M. Leprae |
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Cigar packet arrangement |
M. leprae |
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Causes leprosy |
M. leprae |
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What is the MOD of transmission of leprosy |
1. Skin to skin contact with infected person 2. Droplet from nose and mouth |
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Basis of diagnosis of M. Leprae |
1. Symptomps 2. Observation of lepra cells |
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What is the skin test for leprosy |
Lepromin test |
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To detect AFB in paraffinized tissues |
WADE FITE FARACO |
