Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
10 Cards in this Set
- Front
- Back
|
Knirps mutants show what phenotype?
|
Gap
(Abnormal Cuticle) |
|
|
Knirps research was conducted by whom?
|
Wieschaus, Nusslein-Volhard, and Jackle
|
|
|
Knirps DNA binding domain
|
2 Zn fingers, each with 4 cysteine residues
Resembles nuclear hormone receptor DNA binding domains |
|
|
Technique to study where/when a gene is transcribed
|
In situ hybridization
|
|
|
In situ hybridization overview
|
Digoxigenin-labeled antisense RNA or other probe is hybridized to fixed specimens
AlkPhos-labeled anti-digoxigenin Ab used to visualize probe binding |
|
|
In situ hybridization tells you...
|
Where transcripts are found
NOT about txn complexes at promoter or whether protein is being produced. |
|
|
Knirps and eve gene interaction
|
Knirps represses eve 3 stripe to small area. Knirps mutants have very wide eve 3 stripe.
|
|
|
Knirps repressor type
|
Short-Range
|
|
|
Mutation of cis-elements allows you to study ...
|
regulation of genes controlled by TFs
|
|
|
P-element mediated germline transformation
|
Use transposing element to insert gene of interest (ie- lacZ) just downstream of TF binding site and marker gene (ie- white eye)
Recover mutant offspring (white eyes). Offspring carry inserted gene in chromosome. Analyze for expression of inserted gene. |
