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68 Cards in this Set
- Front
- Back
Cell culture of what virus?
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RSV
(note the syncytia) (Lack of inclusions) |
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Cell culture of what virus?
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Adenovirus
(grape-like clusters of rounded cells) (dark basophilic nuclear inclusions) |
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Cell culture of what virus?
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Measles
(Syncytia) (Large intranuclear eosinophilic inclusions) |
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Cell culture of what virus?
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Mumps
(Syncytia. Indistinguishable from RSV based on CPE - cytopathic effect - but has a hemagglutin protein = RBCs adhere to cell surfae. aka hemadsorption & allows differentiation between mumps & RSV) |
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Which viruses form syncytia?
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RSV
Measles Mumps HSV |
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Cell culture of which virus?
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HSV
-Grows fast in culture (3d) -Plaques of rounded cells -Nuclear inclusions -Formation of giant cells and multi-nucleate syncytia -COWDRY BODIES |
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Virus?
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CMV
Grows only in fibroblasts Slow OWLS EYE NUCLEI Nuclear & cytoplasmic inclusions |
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DNA viruses that are nonenveloped?
enveloped? |
Non: Parvo, Adeno, Papova, Polyoma
Env: Herpes, Pox, Hepadna |
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RNA viruses that are nonenveloped?
enveloped? |
Non: Calici (HEV), Picorna (enterov, HAV, rhino), Reo (Rota)
Env: all the rest |
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Nuclear & cytoplasmic inclusions are seen in what viruses?
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CMV
Measles |
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Nuclear inclusions only?
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HSV
Adeno |
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Cytoplasmic inclusions only?
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Rabies
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EM of what virus?
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Adenovirus
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Virus?
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Rabies
NEGRI BODY (cytoplasmic inclusion) |
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Name the 7 DNA viruses
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Parvo-viridae
Hepadna- Polyoma- Papilloma- Adeno- Herpes- Pox- |
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Which DNA viruses are enveloped?
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Hepadna
Herpes Pox |
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there are 14+ RNA viruses.. which are NOT enveloped?
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Rio / Rota
Bonya / Hanta Picorna (HAV, entero, rhino) Calici (Novo, HEV) Astro |
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Which virus is ssDNA?
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Parvo
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Which virus is dsRNA?
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Rota
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What families are the hepatitis viruses in?
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HAV: Picorna (RNA)
HBV: Hepadna (dsDNA, enveloped) HCV: Flavi (RNA, enveloped) HDV: not included - a funny type HEV: Calici (RNA) |
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Which hepatitis virus is a DNA virus?
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HBV
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2 viruses that have both nuclear and cytoplasmic inclusions
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CMV
Measles |
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– Pneumonia in immunocompromised patients & military recruits
– Acute gastroenteritis children (40,41) – Pharyngitis, pharyngoconjunctival fever – Keratoconjunctivitis – HEMORRHAGIC CYSTITIS – Cervicitis, urethritis – Disseminated disease |
Adenovirus
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Adenovirus
NUCLEAR inclusion only, large basophilic Smudgy and coarse No cytomegaly or multinucleation |
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EM of adenovirus virions
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Shingles (zoster)
Affects one dermatome |
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CMV establishes latency in?
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Macrophages
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CPE of which virus?
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CMV
Grape like clusters of rounded cells in a clean background |
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CPE of VZV
Foci of sandpaper with rounded cells |
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HSV
Rounded cells at the edge |
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What virus causes roseola and what cell does it live in?
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HHV6 (sixth disease)
T CELLS! |
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What virus is the hardiest to transport and is the quickest to grow in cell culture?
Which takes the longest to grow in cell culture? |
HSV (24hrs!)
CMV (14-21d) RSV (10-14d) |
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Adenovirus is latent in?
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Lymphoid tissue
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What strains of adenovirus cause gastroenteritis in kids?
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40 & 41
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HEMORRHAGIC CYSTITIS
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adenovirus
BK virus |
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Fifth disease
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Parvovirus B19
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Bone marrow aspirate. Dx?
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Parvovirus
Mature erythroid precursors are absent, and giant pronormoblasts are typical of B19 infection. |
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Polyomaviruses & associations
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JC - PML
BK - hemorrhagic cystitis |
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Dane particle
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HBV virion
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HBV belongs to what family?
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Hepadnavirus (dsDNA enveloped)
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HBeAg?
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Chronic infection
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HCV belongs to what family?
Which type of HCV is worst? HCV treatment? |
Flaviviridae
Type 1 is worst. IFN & ribavirin |
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What % of HBV becomes chronic?
HCV? |
9% HBV becomes chronic
80% HCV; 25% get cirrhosis |
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Enterovirus
Teardrop & Kite-like cells! |
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Coxsackie A
Coxsackie B |
A: Hand-foot-and-mouth disease
B: Pericarditis, myocarditis |
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Picornaviridae
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Enteroviruses (Polio, coxsackie, enterovirus)
HAV Rhinovirus non-enveloped RNA |
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Orthomyxoviridae
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Influenza
enveloped RNA Helical capsid |
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Antigenic drift
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Minor change in amino acid of H or N. cross-antibody protection
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Antigenic shift
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Major change. Genome rearrangement. "New" virus! No protection
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Subtyping of influenza is based on what?
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H (hemagglutinin) and N (neuraminidase)
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Which influenza type is worse and why?
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Influenza A. Can have antigenic drift or shift!
Influenza B only drifts. |
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Amantidine treats?
Tamiflu treats? |
Amantidine treats Influenza A only
Tamiflu treats Influenza A & B |
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Paramyxiviridae
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Measles
Mumps RSV Parainfluenza enveloped RNA virus |
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Koplik spots of measles
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Atypical measles
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Hypersensitivity reaction that occurs in patients incompletely vaccinated for measles
high fever, headache, cough, and abdominal pain. The rash may appear 1 to 2 days later, often beginning on the limbs. SUBACUTE SCLEROSING PANENCEPHALITIS |
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Croup
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Parainfluenza
Member of paramyxoviridae, enveloped RNA virus |
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Parotitis
testes/ovary eye/ear CNS |
Mumps (paramyxoviridae)
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CPE of RSV
syncytium formation |
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RSV is in what family?
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paramyxoviridae
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Gastroenteritis in a child
fecal specimen |
ROTAVIRUS! (wheels)
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Adult T cell lymphoma
Tropical spastic paraperesis |
HTLV (of the retrovirsuses)
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What type of vaccine is MMR?
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live attenuated
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What family are the arboviruses?
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Trick question!
term used to refer to a group of viruses that are transmitted by arthropod vectors. The word arbovirus is an acronym (ARthropod-BOrne viruses) RNA viruses |
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What mosquitoes transmit WNV, dengue and yellow fever?
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Aedes (A. aegypti)
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maculopapular and progresses to vessicular - all lesions
in same stage on a body area - central body outward |
Smallpox
ALL LESIONS SAME STAGE |
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smallpox
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Rabies
bullet-shaped |
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What family is rabies?
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rhabdoviridae
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