Microbiology Test 1 Notes

Front 1

Which enzyme breaks a carbon-carbon bond in the Embdem-Myerhof Pathway?

Back 1

Aldolase

Front 2

What enzyme electronically rearranges bonds within a molecule and thereby generates new functional groups?

Back 2

Isomerase

Front 3

What is the most accurate determination of bacterial growth?

Back 3

Log of the number of viable bacteria over time

Front 4

What happens during the stationary phase of bacterial growth?

Back 4

The number of dividing and dying bacteria may equal

Front 5

To determine which of two substances the worst bacterial nutrient is, one would most likely:

Back 5

compare growth curves in presence of each substance

Front 6

During the lag phase of bacterial growth, bacterial cell mass and volume:

Back 6

Increase

Front 7

The number of viable bacterial cells increasing by 2-fold per unit, this time is called:

Back 7

Generation time

Front 8

Enzymes are catalysts because they:

Back 8

1) Optimally orient reactants
2) Lower energy of activation
3) Bring reactants closer together

Front 9

The phosphotransferase system of E.coli is how this organism generates this substance in the cytoplasm

Back 9

Glucose-6- PO4

Front 10

In Peptidoglycan, the carboxyl group of lactic acid moiety is covalenty bound to:

Back 10

L-alanine

Front 11

Transpeptidase forms a peptide bond with an opposing strand's carboxyl group of:

Back 11

A terminal D-alanine

Front 12

Which pair of substances would not provide at least one substance necessary for the following reaction?

Back 12

NADH+ H+ and ATP

Front 13

Fatty acids are covalently attached to this substance in the Gram-positive bacterial plasma membrane

Back 13

Glycerol

Front 14

A multi-subunit enzyme alwasy exhibits

Back 14

Non-covalently interacting subunits

Front 15

Disruption of bacterial plasma membrane could affect:

Back 15

Electron Transport

Front 16

If L-lysine is present within peptidoglycan, the following amino acid is not present:

Back 16

Diaminopimelic acid

Front 17

Cytochrome proteins are an integral part of the bacterial?

Back 17

Plasma membrane

Front 18

Electron and energy-yielding process and the final electron acceptor is nitrate (NO3-) ion?

Back 18

Anaerobic respiration

Front 19

A nucleoside is composed of

Back 19

A purine or pyrimidine base with a sugar covalently attached

Front 20

For bacteria the number 70S refers to:

Back 20

The catalalytically active ribsome

Front 21

The transport of a substance against a concentration gradient across the plasma membrane

Back 21

Active Transport

Front 22

Small molecule diffusion across the outer membrane of Gram-negative bacteria depends upon:

Back 22

Porins

Front 23

An electron and energy-yielding process with the final electron acceptor being pyruvic acid

Back 23

Fermentation

Front 24

If bacterium could catabolize ATP to ADP to PO4 3- via ATP synthase, what result may occur?

Back 24

Production of proton motive force

Front 25

How is Proton Motive force established during electron transport and anaerobic respiration?

Back 25

H+ vectored outside the cell via the electron transport system proteins

Front 26

The conversion of nitrogen gas to ammonia is known as:

Back 26

Nitrogren fixation

Front 27

The outer membrane of Gram (-) bacteria has two leaflets, inner and outer, which respectively are:

Back 27

Inner= phospholipid
Outer= LPS

Front 28

An endospore's core may be filled with:

Back 28

Dipicolinic acid

Front 29

The a-helix and b-pleated sheet structures are examples of protein

Back 29

Secondary structure

Front 30

The enzyme that reduces nitrogen gas to ammonia

Back 30

Nitrogenase

Front 31

What generic enzyme could theoretically perform the following reaction?
Glucose-6-PO4  Glyceraldehyde-3-PO4 + Pyruvate

Back 31

Aldolase

Front 32

The enzyme, triose isomerase, converts the following substance into Glyceraldehyde-3-PO4

Back 32

Dihydroxyacetone-PO4

Front 33

Of the ten steps in the Embden-Myerhof pathway, how many involve an oxidation/reduction reaction?

Back 33

1

Front 34

Of the ten steps in the Embden-Myerhof pathway, how many involve a kinase reaction?

Back 34

4

Front 35

Substrate-level phosphorylation involves?

Back 35

Conversion of ADP + PO4
= to ATP by any means other than oxidative phosphorylation

Front 36

Which enzyme co-factor is re-generated during lactic acid formation in glycolysis?

Back 36

NAD+

Front 37

How many moles of CO2 per mole of Glucose are released in the Kreb's cycle, only?

Back 37

4

Front 38

A two-electron transfer with an overall Eo' of –0.316 volts could hypothetically lead to this number of
high-energy phosphate bonds.
Go' = – [ nFEo'] and F = 23,063 cal/deg/mole equivalent

Back 38

0

Front 39

Chemotaxis by bacteria does not involve

Back 39

Recognition of concentration differences along length of cell

Front 40

What could increase the Proton Motive force in bacteria?

Back 40

A higher pH within the cytoplasm relative to the outside of the cell

Front 41

The most abundant protein within the Outer Membrane of Gram-negative bacteria – attaches OM to cell
wall

Back 41

Braun's lipoprotein

Front 42

Hopanoids are:

Back 42

Cholesterol like structures

Front 43

Bacterial chemotaxis requires this bacterial structure

Back 43

Flagellum

Front 44

We did not discuss this bacterial secretion system

Back 44

Sec