Microbiology Lab Review For 022112 Quiz

Front 1

What is minimum growth temperature?

Back 1

Lowest temperature at which growth will occur. Below this temp, enzyme activity is inhibited and cells are metabolically inactive so growth is negligible or absent.

Front 2

What is maximum growth temperature?

Back 2

Highest temp at which growth will occur. Above this temp, most cell enzymes are destroyed and organism dies.

Front 3

What is optimum growth temperature?

Back 3

Temp at which the rate of reproduction is most rapid. However, it is not necessarily optimum or ideal for all enzymatic activities of the cell.

Front 4

What are the three classifications of bacteria (depending on temperature requirements)?

Back 4

Psychrophiles, mesophiles, thermophiles.

Front 5

Bacterial species that will grow in a temp range of -5C to 20C. Distinguishing characteristic is it will grow between 0-5C.

Back 5

psychrophiles

Front 6

Bacterial species that will grow within temp range of 20-45C. Distinguishing characteristics are ability to grow at human body temp (37C), and inability to grow at temps above 45C.

Back 6

mesophiles

Front 7

A mesophile whose optimum growth temperature is in the range of 20-30C are called what?

Back 7

Plant saprophytes

Front 8

A mesophile whose optimum growth temperature is in the range of 35-40C are organisms that prefer what?

Back 8

To grow in the bodies of warm blooded hosts.

Front 9

Bacterial species that will grow at 35C and above.

Back 9

Thermophiles

Front 10

Organisms that will grow at 37C with an optimum growth temperature of 45-60C.

Back 10

Facultative thermophiles.

Front 11

Organisms that will grow only at temperatures above 50C with optimum temperatures above 60C.

Back 11

Obligate thermophiles.

Front 12

True or False: The ideal temperature for specific enzymatic activities may not coincide with the optimum growth temperature for a given organism.

Back 12

True.

Front 13

___________ bacteria are capable of surviving within a broad pH range, which is characteristic of their natural inhabitat, the digestive system.

Back 13

Enteric

Front 14

Bacterial _________ _________ can only tolerate a narrow range. The pH of the circulatory system remains fairly constant at approx. 7.4

Back 14

blood parasites

Front 15

The specific range for bacteria is between _______ and ______, with the optimum being _____ to _____.

Back 15

4 and 9
6.5 to 7.5

Front 16

Fungi, molds, and yeasts prefer an _______ environment with optimum activities at a pH of _____ to _____.

Back 16

acidic
4 to 6.

Front 17

The pH of a laboratory medium is usually adjusted to approx. 7. Why is that?

Back 17

Because a neutral or nearly neutral environment is generally advantageous to the growth of microorganisms.

Front 18

True or False: Metabolic activities of the microorganism will result in the production of wastes and will cause shifts in pH that can be detrimental to growth.

Back 18

True.

Front 19

_______ require the presence of atmospheric oxygen for growth.

Back 19

Aerobes

Front 20

________ require limited amounts of atmospheric oxygen for growth.

Back 20

Microaerophiles

Front 21

_______ require the absence of free oxygen for growth because their oxidative enzyme system requires the presence of molecules other than O2 to act as the final hydrogen acceptor.

Back 21

Obligate anaerobes.

Front 22

These are fermentative organisms and therefore do not use O2 as a final electron acceptor.

Back 22

Aerotolerant anaerobes

Front 23

These organisms can grow in the presence of O2 or in the absence of O2.

Back 23

Facultative anaerobes.

Front 24

O2 Requirement:

Staph. aureus at 37C is considered a:

Back 24

Facultative anaerobe

Front 25

O2 requirement:

Corynbacterium xerosis at 37C

Back 25

Microaerophile

Front 26

O2 requirement:

Enterococcus faecalis at 37C

Back 26

Aerotolerant anaerobe

Front 27

O2 requirement:

Saccharamyces cerevisiae at 25C

Back 27

Facultative anaerobe

Front 28

O2 requirement:

Aspergillus at 25C

Back 28

Obligate aerobe

Front 29

O2 requirement:

Costridium sporogenous at 37C

Back 29

Obligate anaerobe

Front 30

Medias used during effects of temp experiment were:

Back 30

1 TSA plate
1 Saboured Slant used for Saccharomyers.

Front 31

Organisms used for effects of temp experiment were:

Back 31

Serratia,
E coli
Geobacillus
Saccharamyces

Front 32

Medias used during effects of pH experiment were:

Back 32

pH 3 tube
pH 6 tube
pH 7 tube
pH 9 tube

Front 33

Organisms used for effects of pH experiment were:

Back 33

Enterococcus faecalis
E coli
Saccharamyces cerevisiae

Front 34

Media used for the O2 requirement was:

Back 34

Brain heart infusion (BHI)

Front 35

How to prepare the BHI for use:

Back 35

1. Heat to molten
2. Cool to 45C, then innoculate.
3. Roll and cool, incubate.

Front 36

What media was used in cultivating anaerobes experiment?

Back 36

1 Fluid Thioglycollatic broth (fTg)
1 TSA plate

Front 37

What organism was used in the fTg?

Back 37

Clostridium

Front 38

What organisms were used on the plate for cultivating anaerobes?

Back 38

Bacillus, E coli, and Clostridium.