Microbiology Lab Quiz 3

Front 1

selective

Back 1

inhibits or allows growth of organisms

Front 2

differential

Back 2

allow growth of all organisms (color change)

contains a sugar organisms can use

Front 3

if organisms use sugar

Back 3

produce an acid> change inph> change in color

Front 4

fermentation

Back 4

metabolism of sugar

breakdown or utlization of food source

Front 5

selective and differential media

Back 5

mannitol salt agar

macckoney

eosin-methylene blue

Front 6

mannitol salt agar selective

Back 6

salt, only staphyloccocci

Front 7

mannitol salt differential

Back 7

mannitol

yellow color

Front 8

ph in mannitol

Back 8

red

Front 9

macckoney selective

Back 9

crystal violet

inhbits gram +

Front 10

macckoney differntial

Back 10

lactose

fermentors red

Front 11

macckoney ph

Back 11

netural red

Front 12

eosin-methylene blue selective

Back 12

eosin y and methylene blue

inhbits gram +

Front 13

eosin-methylene blue differntial

Back 13

lactose

ecoli-metallic green

e-areogenes- thick pink

Front 14

carbohydrates

Back 14

sugar food source

Front 15

byproducts in carbohydrate fermentation

Back 15

co2 gas- air bubbles

pyruiv acid - color change if ph indicator present

Front 16

TSI test

Back 16

glucose, lactose, sucrose-yellow

h2s produciton- black

Front 17

tsi test method

Back 17

stab and streak

stab butt and draw up from line of insertion and streak on top of slant

Front 18

IMvic tests

Back 18

indole

citrate

Front 19

indole test

Back 19

test for presence of enzyme

tryptophanase- breaks down tryphtophan

SIM deep

+ cherry red

- yellow or black

Front 20

what do you add to indole test

Back 20

kovac's reagent 10 drops

Front 21

citrate test

Back 21

simmons citrate agar slants

+-blue agar

- green

Front 22

can organisms utilize citrate as energy source

Back 22

yes

Front 23

h2s test

Back 23

SIM

+ black

- clear

Front 24

purpose of special purpose media

Back 24

1.isolation of bacteria from a mixed population of organisms

2. differentiation among closley related groups of bacteria

3. enumeration of bacteria in sewage and water and food and dairy

4. assay of naturally occuring substances like anitbotics, vitamins, products of industrial fermentation

5. characterization and identification of bacteria by their abilities to produce chemical changes

Front 25

colliform bacilli

Back 25

e.coli

produces greater quanities of acid from lactose

can ferment lactose

Front 26

dysentery, typhoid, and paratyphoid bacilli

Back 26

not lactose fermentors

Front 27

anerobic

Back 27

without oxygen

Front 28

aerobic

Back 28

with oxygen

Front 29

do all microorganisms use pyruvic acid in the same way

Back 29

no

Front 30

tsi is designed to differentiate among with groups of bacteria

Back 30

enterobacteriaceae

Front 31

why is the acid-base indicator phenol red in a TSI

Back 31

detect carbohydrate fermentation

Front 32

why does TSI contain thiosulfate

Back 32

substrate for h2s production

Front 33

why 18 to 24 hours to view TSI

Back 33

make sure carbohydrate substrate have not been depleted and the degradation of peptones yielding alkaline end products has not taken place

Front 34

enterobacteriaceae

Back 34

found in intestinal tract of humans and mammals

pathogens-

ocassional pathogens

normal intestinal flora

Front 35

tryphtophan

Back 35

essential amino acid

(from food)

Front 36

citrate permenase

Back 36

facillitates transport of citrate in cells

Front 37

citrate is apart of what cycle

Back 37

krebs

Front 38

enzyme of citrate

Back 38

citrase

converted to pyruvic acid and carbon dioxide

Front 39

motility in hydrogen sulfide

Back 39

not restricted to line of incoluation

Front 40

ferrous ammonium sulfate in h2s production

Back 40

indicator combining with the gas forming an insoluble black ferrous sulfide perticipate