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26 Cards in this Set
- Front
- Back
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Syphilis test is performed in what temperature? |
Room Temperature |
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What sample is used in Syphilis test |
1. Whole blood, plasma or serum |
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Syphilis test: If the plasma or serum are not tested immediately, they should be refrigerated at what temperature? |
2-8 degrees Celcius Storage: not more than 2 weeks |
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Syphilis Test: If the plasma or serum contains precipitate, what will you do to the sample? |
Discard |
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Syphilis Test: How many drops of assay diluent will you incorporate to the sample? |
4 drops (120 microliters) |
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Syphilis Test: How many microliters of PLASMA sample will you use? |
10 microliters |
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Syphilis Test: How many microliters of BLOOD will you use in the test? |
20 microliters |
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Syphilis Test: What color is the band in the result window of the test? |
Purple |
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Syphilis Test Interpretation: C: Presence of Purple band T: None |
Negative |
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Syphilis Test Interpretation: C: Presence of Purple band T: Presence of Purple band |
Positive |
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Syphilis Test Interpretation: C:None T: Presence of Purple band |
Invalid Test |
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Syphilis Test Interpretation: C: None T: None |
Invalid test |
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Syphilis Test Interpretation: C: None T: Presence of Purple band |
Invalid test |
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The SD Bioline Syphilis test will indicate the presence of _________ in the specimen. |
Treponema pallidum antibodies |
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What specific antibodies are detected in the Syphilis test? |
IgM, IgG and IgA against Treponema pallidum |
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What is the morphology and gram staining properties of Treponema pallidum? |
Gram negative, spirochetetal bacterium |
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What method can you use to visualize Treponema pallidum in a dark field microscopy |
Silver impregnation method |
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The Treponema pallidum spirochete flagellum moves in what motion? |
Corkscrew motion |
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THe SD Bioline Rapid Syphilis test is what kind of assay? |
Solid phase immunochromatographic assay or TPHA (Treponema pallidum hemagluttination assay) |
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What are the existing confirmatory immunological methods in detecting Treponema pallidum? (Treponemal test) |
1. TPHA (Treponema pallidum hemagluttination assay) 2. FTS-ABS (Fluorescent Treponemal antibody adsorption test) |
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Syphilis Test Once you have placed the sample in the "S" hole, How many minutes after will you add the assay diluent ? |
Within 5-20 min. |
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Syphilis Test: Time allotment for the interpretation of results |
Within 5-20 minutes only. |
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Syphilis Test: Interpretation of the result after 20 mins will yield? |
False results |
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Syphilis Test: If the purple line appears first in the "T" than the "C", the result is true or false postive? |
True positive |
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Syphilis Test: What will you use in collecting blood using the lancet? |
20 micro liters Capillary pipette |
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When collecting whole blood, you need to settle the sample for ____minutes before you centrifuge it to get serum specimen of supernatant |
30 minutes |
