Microbiology Lab Final Exam, Gcc, Summer 2011

Front 1

Hydrogen Sulfide Test is Used to Indicate/ Positive result:

Back 1

Tests for the production of hydrogen Sulfide and motility.

Positive result is indicated by the media turning black. H2S has been produced.

Front 2

Indicator in the Hydrogen Sulfide Test

Back 2

Ferrous Ammonium Sulfate

Front 3

Hydrogen Sulfide Test Media Contains:

Back 3

SIM Tube:

Sulfur substrates: Peptone & Sodium Thiosulfate

Front 4

Enteric Lactose Fermenting Microorganisms

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1. Escherichia coli
2. Enterobacter aerogenes
3. Klebsiella pneumoniae

Front 5

Enteric Non-Lactose Fermenting Microorganisms

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1. Salmonella typhimurium
2. Shigella dysenteriae
3. Proteus vulgaris
4. Pseudomonas faecalis
5. Alcaligenes faecalis

Front 6

Nonenteric Microorganisms

Back 6

1. Corynebacterium xerosis
2. Micrococcus luteus
3. Lactococcus lactis
4. Staphylococcus aureus
5. Bacillus cereus

Front 7

Extracellular Enigmatic Activity Test is Used to:

Back 7

Determine the ability of microorganisms to excrete hydrolytic extracellular enzymes capable of degrading starch, tributryin, casein and gelatin. Macro molecules must be hydrolyzed.

Front 8

Extracellular Enzymatic Activity Test Results:

Back 8

Plate: Positive results were determined by a clearing of the opacity of the media surrounding growth. No change determined a negative result.

Tube: If media was liquid after refrigeration at 4C degrees it indicaed fast gelatin hydrolysis. If it was liquid after 5 more days of growth it was slow gelatin hydrolysis.

Front 9

Extracellular Enzymatic Activity Test Media Used:

Back 9

1. Starch Agar Plate
2. Spirit Blue Agar Plate
3. Milk Agar Plate
4. Gelatin Tube

Front 10

Extracellular Enzymatic Activity Test
Starch:

Back 10

Enzymes: amylase & maltase
Bonds are glycosidic.
End result is glucose.
Media: starch agar plate.

Front 11

Extracellular Enzymatic Activity Test
Lipids:

Back 11

Enzymes: lipase
Bonds are ester bonds.
End result glycerol and fatty acids.
Media: spirit blue agar plate.

Front 12

Extracellular Enzymatic Activity Test
Casein:

Back 12

Enzymes: proteases. Process called proteolysis.
Bonds are peptide bonds.
End result is amino acids.
Media: milk agar plate.

Front 13

Extracellular Enzymatic Activity Test
Gelatin:

Back 13

Enzymes: gelatinase.
End result amino acids.
Media: gelatin deep tube.

Front 14

Carbohydrate Fermentation Test is Used to:

Back 14

Test for the ability of a microorganism to ferment cerbohydrates.

Front 15

Durham Tube:

Back 15

Used to detect gas, C02, formed by acid production.

Front 16

Carbohydrate Fermentation Test Results:

Back 16

Tubes contain phenol red. They will be light red at a pH of 7. Acid production druing fermentation will turn the media yellow, even a small amount, 6.8. A bubble in the Durham Tube indicates gas production during fermentation.

Front 17

The so-called fermenters of carbohydrates:

Back 17

Facultative Anerobes

Front 18

One mole of glucose is converted into:

Back 18

Two moles of pyruvic acid.

Front 19

Cellular Respiration:
Aerobic

Back 19

Biooxidations in which molecular oxygen can serve as the final electron acceptor.

Front 20

Cellular Respiration:
Anaerobic

Back 20

Biooxidations in which inorganic ions other than oxygen, such as a nitrate ion or sulfate ion, can can serve as the final electron acceptors.

Front 21

Fermentation

Back 21

A biooxidative process not requiring oxygen in which an organic substrate serves as the final electron acceptor.

Front 22

IMViC Test Stands For:

Back 22

Indole
Methyl Red
Voges-Proskauer
Citrate Utilization

Front 23

IMViC Test is Used to:

Back 23

Differentiate between the principal groups of Enterobacteriaceae.

1. Enteric Lactose Fermenters
2. Enteric Lactose Nonfermenters
3. Nonenteric

Front 24

Enterobacteriaceae:

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Groups of bacteria that can be found in the in testinal tract of humans and lower mammals. They are short, gram-negative, nonspore-forming, bacilli.

They can be:
1. Pathogens
2. Occasional Pathogens
3. Normal Intestinal Flora

Front 25

Indole Production Test is Used to:

Back 25

Determine the ability of microorganisims to degrade the amino acid tryptophan.

Enzyme: Tryptophanase

Front 26

Indole Production Test:
Test Results

Back 26

Cultures producing a red reagent layer following addition of Kovac's reagent are indole positive, indicating the hydrollyzation of tryptophan.

Front 27

Methyl Red Test is Used to Indicate:

Back 27

Acid production and stabilization following oxidation of glucose.

pH Shift

Front 28

Fermentation=

Back 28

Acid

Front 29

Methyl Red Tests for/ Results:

Back 29

pH Indicator
fermentation
acid

Turns red (oxydize glucose) in acid - pH 4

Turns yellow in weak acid - pH 6

Front 30

Voges-Proskauer Test is Used to Indicate:

Back 30

Determines the ability of some organisims to nonacidic or neutral end products from the organic acids that result from glucose metabolism.

*Characteristic of E. aerogenes*

Front 31

Reagent for Voges-Proskauer Test

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Barritt's Reagents A&B

Front 32

Voges-Proskauer Test Results:

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Developement of a deep rose color in the culture 15 minutes following the addition of Barritt's reagent is indicative of the presence of acetylmethylcarbinol and represents a positive result.

Front 33

Citrate Utilization Test is Used to Indicate:

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Differentiate among enteric organisims on the basis of their ability to ferment citrate as a sole source of carbon. This ability depends on the presence of citrate permease which allows citrate into the cell.

Front 34

Citrate Utilization Test Results:

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The presence of sodium carbonate changes the bromthymol blue indicator incorporated into the media from green to deep Prussian blue.

Front 35

Enzyme in the Citrate Utilization Test:

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Citrase

Front 36

Media for the Citrate Utilization Test:

Back 36

Simmons Citrate Agar Slant Tube

Front 37

Urease Test:
Media

Back 37

Urea Broth Tube

Front 38

Urease Test:
Test Results

Back 38

Media that turns pink/deep pink indicates the production of the enzyme urease.

Urease hydrolyzes urea to form the alkaline end product ammonia.

Front 39

2NH3=

Back 39

Ammonia

Front 40

Urea is an:

Back 40

Amino Acid

Front 41

Nitrate Reduction Test:
Media

Back 41

Nitrate broth tube.

Front 42

NO3=

Back 42

Nitrate

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NO2=

Back 43

Nitrite

Front 44

N2 or NH3=

Back 44

Molecular Nitrogen

Front 45

Nitrate Reduction Test:
Reagents

Back 45

A&B test for NO2/Nitrite.

C= Zinc. Zinc degrades NO3 Nitrate producing NO2 Nitrite.

Front 46

Nitrate Reduction Test:
Test Results NO3 ----- NO3

Back 46

Negative result: Nitrate is not reduced. No reaction with Reagents A&B. Will turn red with the addition of zinc.

Front 47

Nitrate Reduction Test
Test Results NO3 ----- NO2

Back 47

Positive result: media will turn red after the addition of Reagents A&B.

Front 48

Nitrate Reduction Test
Test Results NO3 ----- N2 or NH3

Back 48

Negative result: no color change with any reagents. NO2 was not produced.

Front 49

Oxidase Test
Media

Back 49

Small Purple Discs

Front 50

Oxidase Test is Used to Indicate:

Back 50

If a microorganism is capable of aerobic respiration.

Front 51

H2O2=

Back 51

Hydrogen Peroxide

Front 52

The only two organisms that will test positive with the Oxidase test:

Back 52

Alcaligenes faecalis
Pseudomonas aeruginosa

Front 53

Oxidase Test:
Enzyme

Back 53

Cytochrome Oxidase

Front 54

Oxidase Test:
Test Results

Back 54

Purple/Black is positive. Indicating Cytochrome Oxidase was produced.

Front 55

Indole Reagent

Back 55

Kovac's

Front 56

Methyl Red Indicator

Back 56

Methyl Red

Front 57

pH Indicator in Carbohydrate Fermentation

Back 57

Phenol Red

Front 58

Indicator for starch hydrolysis

Back 58

Iodine

Front 59

Lipid substrate in Spirit Blue media

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tributyrin

Front 60

Indicator/Substrate for casin hydrolysis

Back 60

casein

Front 61

What does catalase do

Back 61

degrade hydrogen peroxide, releasing oxygen

Front 62

Positive indicator in catalase test

Back 62

bubbles

Front 63

Gas released in catalase test

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O2 - oxygen

Front 64

what is the reagent in the catalsae test

Back 64

3% hydrogen peroxide

Front 65

What is urease

Back 65

Enzyme

Front 66

What is the pH indicator in the urease test

Back 66

phenol red

Front 67

What is the enzyme in the nitrate reduction test

Back 67

nitrate reductase

Front 68

decarboxylation

Back 68

removal of a carboxal from an amino acid

Front 69

amino acid utilization positive test part A

Back 69

LD+ purple / LD- yellow

Front 70

amino acid utilization positive test result means

Back 70

l-lysine has been decarboxylated

Front 71

amino acid utilization part A reagent

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bromcresol purple

Front 72

amino acid utilization part B reagent

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10-12% ferric chloride solution

Front 73

amino acid utilization part B media

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phenylalanine slant tube

Front 74

amino acid utilization part B positive test result/color

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changes to green
phenylalanine deaminase was present

Front 75

What does the taxonomic classification of protozoa depend on

Back 75

locomotion/ motility

Front 76

what are the taxonomic classifications of protozoa

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1. sarcodina
2. mastigophora
3. ciliophora
4. sporoza (apocomplexa)

Front 77

What causes maleria

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plasmodium (sporozoa)

Front 78

pellicle

Back 78

cell membrane support

Front 79

pseudopods

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(false feet) locomotion

Front 80

oral groove

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eating

Front 81

micronucleus

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conjugation

Front 82

eye spot

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sense light

Front 83

mycelium

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intertwining branching mat of fungi

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hyphae

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the filiments that make up the mycelial mat

Front 85

arial hyphae

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mycelium that raises up from the mat

Front 86

how do yeasts reproduce asexually

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fission

Front 87

how do yeasts reproduce sexually

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conjugation

Front 88

bakers yeast

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saccharomyces cerevisiae

Front 89

diomorphism

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y-m shift
yeast to mold

Front 90

vegetative hyphae

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spores

Front 91

sporagiophore

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sporagiospores

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conidiophore

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conidia

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oidiophore

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oidia

Front 94

spores forming in hyphae

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chlamydospore

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ascus

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ascospores

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basidium

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basidiospores