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11 Cards in this Set
- Front
- Back
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What are spores?
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Spores-
Bacteria produce metabolically INACTIVE, DORMANT forms of bacterial cells |
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What are endospores?
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Endospores-ACTIVE
Spores that develop within the vegetative bacterial cells |
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What are the functions of endospores?
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(1) are not part of the reproductive cycle
(2) Help bacteria to SURVIVE harsh environmental conditions--until normal growth is restored. (3) classify bacteria as SPORE-FORMING or NON-SPORE-FORMING. |
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Can spore production be considered a form of reproduction?
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NO
A spore germinates into a vegetative cell. There is no net increase in cell number. |
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Name at least two diseases caused by spore-forming bacteria?
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(1) Anthrax
(2) Botulism (Clostridium botulinum) (3) tetanus (Colstribium tetani) (4) Gas gangrene |
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What is the most common staining technique for viewing spores?
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Schaeffer-Fulton Method
Because of endospores structural and chemical nature they are difficult to stain by conventional staining techniques (once stained it's DIFFICULT to DECOLORIZE them) |
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What is Positive staining for spores and it's active ingredient and appearance.
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Uses Schaeffer-Fulton method
ACTIVE INGREDIENT: Malachite Green APPEARANCE: (1) Endospores-pick up malachite green and appear GREEN (2) Vegetative cells-appear RED due to safranin |
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What is Negative staining for spores and it's active ingredient and appearance.
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Uses Crystal Violet Method
ACTIVE INGREDIENT: Crystal Violet APPEARANCE: (1) Endospores-are COLORLESS (2) Vegetative cells-appear PURPLE |
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Identify this type of spore.
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Central spore (center)
A=Spore B= bacterial vegetative cell |
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Identify this type of spore
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Subterminal Spore (closer to one end)
A=Spore B= bacterial vegetative cell |
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Identify this spore
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Terminal Spore (at the end)
A=Spore B= bacterial vegetative cell |
