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24 Cards in this Set
- Front
- Back
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Q: What is the function of the base and arm of a microscope?
(Lab Exercise 1-Microscope) |
A: Base and Arm-
these form the basic frame of the microscope and are used to hold the microscope during transport |
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Q: What is the function of the stage in a microscope?
(Lab Exercise 1-Microscope) |
A: Stage-
this part is designed to hold the slide with the help of clips. The slide can be moved by manipulating the two slide positioning knobs located just below the stage. |
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Q: What is the function of the sub stage condenser?
(Lab Exercise 1-Microscope) |
A: Sub stage Condenser-
(located just below the hole of the stage) It has a lens that concentrates and focuses light onto the object |
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Q: What is the function of the Pinion Knob of a microscope? It works in conjunction with that other part?
(Lab Exercise 1-Microscope) |
A: Pinion Knob-
this is a knob that can move the condenser to adjust brightness and contrast |
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Q: What is the function of the Iris Diaphragm of a microscope?
(Lab Exercise 1-Microscope) |
A: Iris Diaphragm-
this device controls the amount of light going to the object, It can be adjusted by using the iris diaphragm lever. |
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Q: What is the function of the Revolving nosepiece of a microscope?
(Lab Exercise 1-Microscope) |
A: Revolving Nosepiece-
this part holds the objective lenses. By rotating the nosepiece, objectives can be switched. |
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Q: What is the function of the Objective lenses of a microscope?
(Lab Exercise 1-Microscope) |
A: Objective lenses?
these are lenses attached to the revolving nosepiece that magnify the image of the object. (There are usually 3-4 objectives on a microscope) |
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Q: What is the function of the Course adjustment knob of a microscope?
It is used with which objective lenses? (Lab Exercise 1-Microscope) |
A: Course adjustment knob-
this knob us used to rapidly move the body tube or the stage up or down in order to focus on to the object. This knob us used for focusing when the scanning or low power objective is used. |
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Q: What is the function of the Fine adjustment knob a microscope?
It is used with which objective lenses? (Lab Exercise 1-Microscope) |
A: Fine Adjustment knob-
(this is the smaller of the two adjustment knobs). It moves the body of the tube or the stage very gradually and is used for fine focusing the high dry and immersed objectives |
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Q: What is the function of theOculars/Eyepiece of a microscope?
(Lab Exercise 1-Microscope) |
A: Oculars/Eyepieces-
these are two lenses which usually magnify the image 10x and are used for looking into the microscope to view the image of the object. |
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Q: What is the function of the Illuminator/Light Source on a microscope?
(Lab Exercise 1: Microscope) |
A: Illuminator/Light Source-
this is the electric bulb in the base of the microscope which provides the light for observing the magnified image of the object |
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Q: Define magnification?
(Lab Exercise 1-Microscope) |
A: Magnification-
to enlarge the image of an object |
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Q: Define resolution?
(Lab Exercise 1-Microscope) |
A: Resolution-
to separate or distinguish structures that are too close together. |
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Q: What is the magnification of the Ocular lenses?
(Lab Exercise 1-Microscope) |
A: Objectives lenses = (10x)
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Q: What is the magnification of the Scanning objectives
(Lab Exercise 1-Microscope) |
A: Scanning objectives = (4x)
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Q: What is the magnification of Low Power objectives?
(Lab Exercise 1-Microscope) |
A: Low Power Objective = (10x)
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Q: What is the magnification of High dry objective?
(Lab Exercise 1-Microscope) |
A: High Dry objective = (40x)
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Q: What is the magnification of Oil-Immersion objective?
(Lab Exercise 1-Microscope) |
A: Oil-Immersion objective = (100x)
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Q: What is total magnification and how is it calculated?
(Lab Exercise 1-Microscope) |
A: Total Magnification
The power of the objective used and the power of the ocular lens combined To calculate: Objective Lens x Ocular lens = total magnification |
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Q: What is the difference between magnification and resolution?
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A: Magnification is used to ENLARGE the image of an object and resolution is used to help DISTINGUISH two objects that are close together.
(Lab Exercise 1-Microscope-Question 3) |
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Q: What is the purpose of placing immersion oil on the slide?
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A: PURPOSE:
(1) allows GREATER amount of magnification (2) oil allows for a cone of light to be DRAWN UP through the objective lens and reflected to the eye by INCREASING the amount of light (rather than scattering and decreasing resolution). (Lab Exercise 1-Microscope-Question 4) |
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Q: What are the advantages of electron microscopes as compared to light microscopes?
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A: Electron microscopes uses an electron beam rather than light to view organisms
(2) It also has the ability to to view organisms at VERY HIGH magnification (Lab Exercise 1-Microscope-Question 5) |
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Q: Give at least 5 major differences between prokaryotic and eukaryotic cells.
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A:
PROKARYOTIC vs. EUKARYOTIC: 1. (P) are ALL microorganisms / (E) only some are 2. (P) No nucleus / (E) has a nucleus 3. (P) cell wall present / (E) no cell wall 4. (P) no mitochondria / (E) have mitochondria 5. (P) chromosome present / (E) no chromosomes present (Lab Exercise 1-Microscope-Question 6) |
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Q: If the ocular magnification is 10x and the oil immersion objective is in place, what is the total magnification?
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A: Total magnification=
(Ocular - 10x) (Oil immersion- 100x) = 1000x magnification |
