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15 Cards in this Set
- Front
- Back
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What was the bacteriophage used and what bacteria can it infect?
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T4r
E. coli strain B |
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What agar was used for the Henrici culture?
Which organisms were used? Which dye was used to visualize them? |
Potato Dextrose Agar
Penicillin notatum Rhizopus stolonifer Lactoaniline blue |
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Fungi can be saprophytes or parasites: T/F
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T
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Fungi forms can be broken into two groups:
What is it called when a fungus species can exist as either form? What are some uses of fungi? |
Mold - multi-cellular and develop into filaments called hyphea
Yeast - unicellular fungi that reproduce asexually Dimorphism beer, wine, cheese, baked goods, antibiotics |
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In the yogurt lab, what are the two organisms and which type of bacteria are they with regards to temperature needs? What temperature are they incubated at?
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Streptococcus thermophilus - thermophile, 47C
Lactobacillus bulgaricus - mesophile, 14C |
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Describe the Antimicrobial susceptibility lab.
Organisms Plate Standard Name of Test |
Organisms: E. coli and S. aureus
Plate: Mueller-Hinton Standard: McFarland Name of Test: Kirby-Bauer |
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Describe the results of blood hemolysis test.
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α - Streptococcus pneumoniae
β - Staphylococcus aureus Streptococcus pyogenes γ - Staphylococcus epidermidis |
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Describe the results of catalase test.
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Staphylococci produce catalase, bubbling occurs in the peroxide
Streptococci do not produce catalase, no bubbling |
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Describe the results of MSA test.
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Streptococci do not grow with high salt concentration
Staphylococci will grow on high salt concentration. S. epidermidis - cannot ferment mannitol, growth with no change to media S. aureus - ferment mannitol, growth with the media changing to yellow |
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Is MSA selective, differential or both?
Is blood agar selective, differential, or both? |
MSA is both
Blood agar is differential |
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Describe MSA's contents, what it selects for, what it differentiates, what mannitol fermentation looks like.
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MSA contains 7.5% NaCl which does not permit he growth of most bacteria except the genus Staphylococcus. Differentiates between organisms that ferment mannitol or not. The phenol red indicator will turn yellow at a pH of 6.8 or below (when mannitol is fermented), remain red at pH 7.4-8.4 (no mannitol is fermented) and pink at pH 8.4 or higher.
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Describe the results of the McConkey agar.
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Coliforms - bright pink colonies
Paracolons - colorless colonies |
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Describe the results of Eosin Methylene Blue (EMB) agar.
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Coliforms - appear deep purple
Paracolons - colorless colonies yet appear pink/purple due to the media E. coli and K. pneumoniae make dye go to the outside of the cells which makes it appear blue-black with metallic green sheen |
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Describe the results of the Salmonella-Shigella (SS) agar.
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Coliforms - ours are mostly inhibited. Pink colonies if present
Paracolons - colorless Salmonella is able to reduce the sodium thiosulfate to hydrogen sulfide which then reacts with the ferric ions in the medium, creating a black center in the colonies |
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Describe the results of the TSI experiment.
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Coliforms:
Slant: Acidic Butt: Acidic Gas: +/- H2S: - Paracolons: Slant: Alkaline Butt: Acidic Gas: - Salmonella typhinurium is the only paracolon which produces H2S Yersinia enterocolitica does not produce H2S |
