Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
171 Cards in this Set
- Front
- Back
- 3rd side (hint)
|
Causative agents of meningitis |
AIMS
Streptococcus agalactiae Haemophilus influenzae Neisseria meningitidis Streptococcus pneumoniae |
AIMS |
|
|
Temperature and time requirement for the flash method of Pasteurization |
72 degrees celcius for 15 seconds |
|
|
|
Temperature and time requirement for batch method of Pasteurization |
63 degrees celcius for 30 minutes |
|
|
|
Temperature, time and pressure of autoclave for sterilization |
121 degrees celcius for 15 minutes, under 15 psi |
|
|
|
Temperature, time and pressure of autoclave for decontamination |
132 degrees celcius for 30 to 60 minutes, under 15 psi |
|
|
|
Temperature for incineration |
870 to 960 degrees celcius |
|
|
|
Temperature and time requirement for dry heat oven |
160 to 180 degrees celcius for 1 and a half hours |
|
|
|
Causative agent of rickettsialpox |
R akari |
|
|
|
Causative agent of trench fever |
Rochalimea quintana, now Bartonella quintana |
|
|
|
Causative agent of scrub typhus |
Orientia tsutsugamushi |
|
|
|
Causative agent of endemic typhus |
Rickettsia typhi |
|
|
|
Other name for endemic typhus |
Murine typhus |
|
|
|
What organisms do not grow on MacConkey Agar? |
Pasteurella Bordetella Francisella Legionella Brucella Gardnerella |
|
|
|
Metachromatic granule stains |
LAMB Loeffler's alkaline Methylene blue Burke's modified Gram stain |
LAMB |
|
|
Flagellar stains |
Leifson Gray Fisher & Conn |
|
|
|
What do flagellar stains contain? |
Tannic acid |
|
|
|
Bacitracin is also known as |
Taxo A |
|
|
|
Bacitracin is produced by |
Bacillus subtilis var Tracy |
|
|
|
Action of Bacitracin when used for Streptococcus |
Differentiates beta hemolytic Group A |
|
|
|
Action of Bacitracin when used for Staphylococcus |
Differentiates Staphylococcus from Micrococci |
|
|
|
Capnophilic requirement |
5 to 10% CO2 |
|
|
|
Microaerophilic requirements |
85% nitrogen 5-10% CO2 5% oxygen |
|
|
|
Causative agents of keratitis |
APA Acanthamoeba & Pseudomonas aeruginosa |
APA |
|
|
DNAse positive organisms |
SMS AS
Staphylococcus aureus Moraxella Serratia Aeromonas Stenotrophomonas maltophila |
SMS AS |
|
|
Culture: BAP flooded with E coli |
Acanthamoeba & Naegleria |
|
|
|
Obligate aerobe |
Grows only in the presence of oxygen |
|
|
|
Obligate anaerobe |
Grows only in the absence of oxygen |
|
|
|
Facultative aerobes |
Anaerobes that can grow in the presence of oxygen |
|
|
|
Facultative anaerobes |
Aerobes that can grow in the absence of oxygen |
|
|
|
2 tests to differentiate Group D enterococcus from Group D non-enterococcus |
PYR Test 6.5% NaCl |
Group D enterococcus are positive to both. |
|
|
Resistant to 0.04 Bacitracin, gram positive cocci in clusters, catalase positive, non-motile |
Staphylococcus |
|
|
|
What does ATCC stand for? |
American Type Culture Collection |
|
|
|
Coagulase negative, DNAse negative, cannot ferment in MSA, susceptible to Novobiocin |
S epidermidis |
|
|
|
Method for preservation of protozoans in stool |
Floatation method |
|
|
|
Specific gravity of zinc sulfate |
1.18 |
|
|
|
Floatation method for Cryptosporidium parvum |
Sheather Sugar Floatation |
|
|
|
What is FECT? |
Formalin-Ether Concentration Technique |
|
|
|
Culture media for Francisella tularensis |
Blood Glucose Cystine Agar (BGCA) |
|
|
|
Culture media for Brucella abortus |
Castaneda medium |
|
|
|
Specimens for Brucella |
Blood and bone marrow |
|
|
|
Culture media for Listeria monocytogenes |
MacBride medium |
|
|
|
Culture media for Gardnerella vaginalis |
Human Blood Tween Bilayer Agar |
|
|
|
Culture media for Clostridium difficile |
Cycloserine Cefoxitin Fructose Agar (CCFA) |
|
|
|
Other name for Klebsiella pneumoniae |
Friedlander's bacillus |
|
|
|
Other name for Haemophilus influenzae |
Pfeiffer's bacillus |
|
|
|
Other name for Brucella abortus |
Bang's bacillus |
|
|
|
Other name for Corynebacterium diphtheriae |
Kleb-Loeffler's bacillus |
|
|
|
Other name for Shigella flexneri |
Strong's bacillus |
|
|
|
Gram negative cocci |
Branhamella/Moraxella Neisseria Veilonella |
There are only 3 species |
|
|
Organisms that grow at 42 degrees celcius |
CPA
Campylobacter Pseudomonas aeruginosa |
CPA |
|
|
Biologic indicator for oven method of sterilization |
Bacillus subtilis var niger |
|
|
|
Biologic indicator for gas chamber |
Bacillus subtilis var globijii |
|
|
|
Biologic indicator for autoclave |
Bacillus steatothermophilus |
|
|
|
Biologic indicator for ionizing radiation |
Bacillus pumilis |
|
|
|
Organisms obtained through nasopharyngeal swab |
Neisseria meningitidis Bordetella pertussis Haemophilus influenzae MRSA |
|
|
|
Culture media for MRSA |
Mueller-Hinton Agar with 4% NaCl |
|
|
|
Organism that exhibits a double-zone hemolysis |
Clostridium perfingens |
|
|
|
Exhibits an alpha-prime hemolysis |
Streptococcus viridans |
|
|
|
Inner - beta hemolysis, outer - alpha hemolysis |
Double-zone hemolysis |
|
|
|
Inner - alpha hemolysis, outer - beta hemolysis. Caused by refrigeration of plate. |
Alpha-prime hemolysis |
|
|
|
Causes gas gangrene or myonecrosis, is lecithinase positive, positive for Naegler's test, positive for Reverse CAMP and exhibits double-zone hemolysis |
Clostridium perfringens |
|
|
|
Potassium cyanide (KCN) positive organisms |
Klebsiella Citrobacter Enterobacter |
|
|
|
Largest DNA virus |
Poxvirus |
|
|
|
Smallest DNA virus |
Parvovirus |
|
|
|
Smallest RNA virus |
Picornavirus or Enterovirus |
|
|
|
Largest RNA virus |
Paramyxovirus |
|
|
|
Semilunar bodies |
Plasmodium species |
|
|
|
Asteroid bodies |
Sporothrix shenkii |
|
|
|
Sclerotic bodies |
Phaeohypomycosis |
|
|
Donovan bodies |
Granuloma inguinale |
|
|
|
Guarneri bodies |
Smallpox |
|
|
Negri bodies |
Rabies |
|
|
|
Cowdry bodies |
Herpes simplex virus |
|
|
|
Most abundant normal flora of the throat |
Streptococcus viridans |
|
|
|
Most common pathogen of the throat |
Streptococcus pyogenes |
|
|
|
ONPG stands for |
Ortho-Nitrophenyl Galactopyranoside |
|
|
|
Test for lactose fermenters, especially late lactose fermenters |
ONPG |
|
|
|
Positive color result for ONPG test |
Yellow |
|
|
|
Acid fast organisms |
Nocardia (partial) Legionella micdadei (partial) Cryptosporidium Isospora MTB |
|
|
|
Components of endotoxins |
Lipid A Lipopolysaccharide O Antigen |
|
|
|
Manifestation of Erythema Chromicum Migrans as seen in Lyme disease |
Bull's-eye rash |
|
|
|
Vector of Lyme disease |
Ticks (Ixodes scapularis) |
|
|
|
Causative agent of Lyme disease |
Borrelia burgdorferi |
|
|
|
Stains for spirochetes |
Levaditi Fontana-Tribondeau Warthin-Starry stain |
|
|
|
Causative agent of rubella |
Togavirus |
|
|
|
Rubella is also known as |
German measles |
|
|
|
Spots found in rubella infection |
Forschheimer spots |
|
|
|
Causative agent of typhoid fever |
Salmonella typhi |
|
|
Rose-colored spots are characteristics of |
Typhoid fever |
|
|
|
Rubeolla is also known as |
Measles |
|
|
|
Spots seen in rubeola |
Koplik's spots |
|
|
Slappy-cheek rash is caused by |
Parvovirus B-19 |
|
|
|
Keratin-loving fungi are known as |
Dermatophytes |
|
|
|
All dermatophytes colonize |
Skin |
|
|
|
Dermatophyte having many micro/macroconidia |
Trichophyton |
|
|
|
Dermatophyte having no microconidia |
Epidermophyton |
|
|
|
What dermatophyte colonize both the skin and hair? |
Microsporum |
|
|
|
Epidermophyton colonize the |
Skin and nails |
|
|
|
What do trichophyton colonize? |
Skin, hair and nails |
|
|
|
Skin test for Corynebacterium diphtheriae |
Schick's test |
|
|
|
Skin test for Chlamydia trachomatis |
Frei's test |
|
|
|
Skin test for antibodies against pneumococci |
Francis test |
|
|
|
Skin test for MTB |
Mantoux test |
|
|
|
Dick's test is a skin test for |
Streptococcus pyogenes |
|
|
|
Skin test for Burkholderia mallei |
Mallein's test |
|
|
|
Non-motile organisms |
SKY
Shigella Klebsiella Yersinia |
SKY |
|
|
Medium for the enhancement of growth of organisms |
Enrichment medium |
|
|
|
Medium with supplements added for fastidious organisms |
Enriched medium |
|
|
|
Media which differentiates organisms based on their biochemical characteristics |
Differential media |
|
|
|
Selective media contain |
Inhibitors |
|
|
Phenylethyl Alcohol Agar (PEA) is an example of |
Selective media |
|
|
Chocolate agar is an example of |
Enriched agar |
|
|
|
Examples of media which are both selective and differential |
Mannitol Salt Agar (MSA) MacConkey Agar (MAC) Eosin Methylene Blue (EMB) Hektoen Enteric Agar (HEA) |
|
|
|
Causative agent of Glander's disease |
Burkholderia mallei |
|
|
|
Causes children's gastroenteritis and winter season gastroenteritis |
Rotavirus |
|
|
|
What is SPS? |
Sodium Polyanethol Sulfonate |
|
|
|
How much SPS is contained in a blood culture bottle? |
0.025% |
|
|
|
How long must Brucella be incubated? |
3-4 weeks |
|
|
|
How long must Leptospira be incubated? |
6-8 weeks |
|
|
|
For most species, incubation period in a blood culture bottle is how long? |
7 days or 1 week |
|
|
|
Specimen of choice for MRSA |
Nasopharyngeal swab |
|
|
|
Specimen of choice for Legionella pneumophila |
Bronchial washing or expectorated sputum |
|
|
|
Specimen of choice for Streptococcus pyogenes |
Throat swab |
|
|
|
Specimen of choice for Clostridium difficile |
Stool |
|
|
|
Clostridium difficile causes |
Pseudomembranous colitis |
|
|
|
Causative agent of subacute bacterial endocarditis (SBE) |
Streptococcus viridans |
|
|
|
Giardia lamblia causes |
Steatorrhea |
|
|
|
Causative agent of lumpy jaw |
Actinomyces (Actinomyces israelii) |
|
|
|
Burkholderia pseudomallei causes |
Mellioidosis |
|
|
|
Causative agent of pseudomembranous colitis |
Clostridium difficile |
|
|
|
Which is indole positive: P mirabilis or P vulgaris? |
Proteus vulgaris |
|
|
|
Thin, tear-shaped microconidia with long, smooth, cylindrical macroconidia |
T rubrum |
|
|
White to pink fluffy colonies with red wine reverse |
T rubrum |
|
|
|
Culture media for T rubrum |
Saboraud Agar |
|
|
|
Tests for diagnosis of Lyme disease |
Silver impregnation techniques and enzyme immunoassay (EIA) |
|
|
|
Also known as arthropod-borne viruses |
Arboviruses |
|
|
|
The 3 Arboviruses |
Bunyavirus Togavirus Flavivirus |
|
|
|
Epstein-Barr virus is associated with |
Burkitt's lymphoma |
|
|
|
B-cell receptor for Epstein-Barr virus |
CD21 |
|
|
|
Causative agent of Rocky Mountain Spotted Fever (RMSF) |
Rickettsia rickettsii |
|
|
|
Rickettsia prowazekii causes |
Epidemic typhus |
|
|
|
Causative agent of Q Fever |
Coxiella burnetii |
|
|
|
K in OX-K stands for |
Kingsburry |
|
|
|
Test for rickettsial infection |
Weil-Felix test |
|
|
|
Microorganisms used as reagents for Weil-Felix test |
Proteus species (Proteus vulgaris & Proteus mirabilis) |
|
|
Test tube brush appearance |
Erysipelothrix rhusiopathiae |
|
|
|
Erysipelothrix rhusiopathiae causes |
Erysipeloid |
|
|
|
Causes erysipelas |
S pyogenes |
|
|
|
Causes erythrasma |
C minutissimum |
|
|
|
QC for DNAse production |
Serratia marcecens & E coli |
|
|
|
QC for deamination activity |
P mirabilis & E coli |
|
|
|
QC for oxidase production |
P aeruginosa & E coli |
|
|
|
QC for motility |
K pneumoniae & E coli |
|
|
|
Most dangerous pathogenic fungi |
Coccidioides immitis |
|
|
|
Causative agent of San Joaquin Valley Fever |
Coccidioides immitis |
|
|
|
Test for T gondii |
Sabin-Feldman dye test |
|
|
|
Causes acute febrile rash in immunocompetent children and aplastic anemia in AIDS patients |
Parvovirus |
|
|
|
Iodine + detergent |
Iodophor |
|
|
|
Iodine + alcohol |
Iodine tincture |
|
|
|
Meaning "cannot ferment sugar" |
Assacharolytic |
|
|
|
Lung migration of Ascaris lumbricoides causes what syndrome? |
Loeffler syndrome |
|
|
|
Parasitic cause of malabsorption and steatorrhea |
Stongyloides stercoralis |
|
|
|
Gram negative coccobacillus associated with cat scratch/bite and is the agent of pasteurellosis, with a name translated as "many killings". |
Pasteurella multocida |
Has a mousy odor according to Theriot. |
|
|
Causes swimming pool granuloma |
Mycobacterium marinum |
|
|
|
Bergey's classification is based on |
Temperature requirements |
|
|
|
Smith & Brown's classification is based on |
Hemolytic patterns |
|
|
|
Lancefield classification is based on |
Carbohydrate composition of cell wall |
|
|
|
Arrowhead hemolysis on CAMP test |
Streptococcus agalactiae |
|
|
|
Culture media for S agalactiae |
Granada medium |
|
|
|
Lancefield classification of S agalactiae |
Group B |
|
|
|
Also known as Taxo P |
Optochin |
|
