Microbiology: Cultivation And Metabolism

Front 1

T/F Obligate anaerobes transform energy be anaerobic respiration OR fermentation

Back 1

True

Front 2

T/F: Aerotolerant anaerobes can grow in the presence of Oxygen, and there for are NOT obligate fermenters

Back 2

FALSE

They are OBLIGATE FERMENTERS

Front 3

These anaerobes use aerobic OR anaerobic respiration OR fermentation

They grow best in the presence of Oxygen, but can grow without it

Back 3

Faculative Anaerobes

Front 4

At what pH do

Acidophiles

Neutrophiles

Alkalophiles

...grow best?

Back 4

Acidophiles : below pH 5.5

Neutrophiles : pH 6-8

Alkalophiles : above pH 8.5

Front 5

This bacteria can tolerate up to 20% salt

Back 5

Staphylococcus Aureus

Front 6

T/F Halophiles require NaCl for growth

Back 6

True

Front 7

through which mechanism does Microbial growth occur?

Back 7

Binary Fission

Front 8

This is the time required for a bacterial population to DOUBLE IN NUMBER

Back 8

Generation Time

Front 9

What is the first step in identification of the organism and Diagnosis?

Back 9

Isolation of the pathogen

Front 10

What is the 2nd step in identification of an organism

Back 10

Obtain a PURE culture

Front 11

This type of media inhibits growth of specific types of organisms by the addition of certain chemicals or dyes

Back 11

Selective

Front 12

This type of media is Nutrient rich so Most bacteria can grow

Back 12

Nutrient/General Purpose

Front 13

In this type of media, you can see visual changes in the medium or a difference in the appearance of colonies

Back 13

Differential Media

Front 14

This type of Media shows certain types of bacterial growth are ENHANCED

Back 14

Enrichment

Front 15

This form or growth measurement utilizes LIGHT REFRACTION and is preformed on liquid cultures

Back 15

Optical Density

Front 16

T/F in CFU, Each colony represents a SINGLE cell

Back 16

True

Front 17

This is the metabolic process that involves the COMPLETE OXIDATION of substrate molecules and the generation of energy

Back 17

Cellular Respiration (aerobic/anaerobic)

Front 18

This is the PARTIAL OXIDATION of metabolites to produce energy in the ABSENCE OF OXYGEN

Back 18

Fermentation

Front 19

T/F: BOTH cellular respiration and fermentation begin with glycolysis

Back 19

TRUE

Front 20

What are the products of Cellular respiration?

Back 20

CO2 and H2O

Front 21

What are the products of fermentation

Back 21

Organic waste products (acid, alcohol)

Front 22

In aerobic respiration, the FiNAL electron acceptor is ___

Back 22

Oxygen

Front 23

In Anaerobic Respiration
(glycolysis--> TCA--> e-transport),

the final electron acceptor is _____

Back 23

an Inorganic Molecule containing oxygen

ex. NO3-, NO2-

Front 24

In the Aerobic Process of Fermentation, the final electron acceptor is _____

Back 24

An organic Molecule

acid or alcohol

Front 25

T/F: The TCA cycle is the @nd step in aerobic, anaerobic repiration and fermentation

Back 25

FALSE

TCA Cycle NOT used in fermentation

Front 26

In bacteria, ATP is made as H+ corsses this cell structure.

Back 26

Cytoplasmic Membrane

Front 27

T/F: The only ATP yielding reactions occur in glycolysis

Back 27

True

Front 28

What is an essential function of Fermentation?

Back 28

regeneration of NAD+ for use in glycolysis

Front 29

T/F: A major benefit of Fermentation is allowing ATP production to continue in the absence of oxygen

Back 29

True

Front 30

T/F: Anaerobic respiration does NOT mean in the absence of oxygen

Back 30

True

it means that the final e- acceptor is an oxygen-containinf molecule, NOT O2

Front 31

This enzyme makes H202

Back 31

Superoxide dismutase

Front 32

This enzyme converts H202 to H20 + o2

Back 32

Catalase

Front 33

This species is known to spoil food in the refrigerator.

its optimal growth range is 10-20 degrees C.

Back 33

Psychrophiles

Front 34

These anaerobes concider Oz to be TOXIC.

The presence of oxygen either kills the cells or inhibits their growth

Back 34

Obligate anaerobes