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15 Cards in this Set
- Front
- Back
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What is the primary stain in an endospore slide? |
Malachite Green |
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What is the counterstain in an endospore slide? |
Safranin |
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Why is heat used to stain bacterial endospores? |
The cell wall is thick and impermeable and resistant to certain dies. Heat helps the dyes and stains to penetrate the wall. |
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What is meant by the term vegetative cell? |
A normal adult cell. |
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What pathogen can be presumptively identified using the acid fast stain? |
Mycobacterium tuberculosis. |
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Why can you not see flagella on smears stained with the usual staining technique? |
They are below the limit of the light microscope's resolution. |
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What is the principle behind the negative staining technique? |
To see capsules, determine cell size and arrangement. |
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What are some structures that may be observed with a negative staining technique? |
Capsules, Spirochetes and dental plaque. |
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What stains vegetative cells in the endospore stain? |
Safranin. |
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What stains the background in the negative stain? |
Nigrosin or India ink. |
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What is the primary stain for the acid fast stain? |
Carbol Fuchsin |
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The acid fast stain is a ______________ stain used to identify acid-fast organisms such as members of the genus __________________. |
differential, Mycobacterium |
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Acid-fast organisms have nearly ________________ cell walls which make them highly _____________ to disinfectants and dry conditions. |
impermeable, resistant |
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The primary stain in acid-fast staining is called ___________ ___________. It is_________-_________ and contains ___________. |
Carbol Fuchsin, lipid-soluble, Phenol |
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Sporulation maybe triggered by... |
Depletion of nutrients, change in temperature or adverse environmental conditions. |
