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60 Cards in this Set

  • Front
  • Back

The functional unit of genetic information

Gene

Super coming is important for DNA structure because?

It makes DNA smaller so that it can fit inside the cell

Plasmids

-small circular DNA found in bacteria and eukaryotes.


- Different sizes


- aren't essential to bacteria but they help with survival

Chromosomes

- are in the nucleus


-only seen during interphase


- Chromosomes are two strands of DNA


- covered in proteins


- Carries genetic information

DNA replication always proceeds in only one direction because the ________ of the incoming nucleotide is attached to the free _____ of the growing DNA strand.

5' and 3'

The template for RNA polymerase is?

a strand of DNA

The new RNA chain is _____ to the template.

Anti parallel

IS the process of transcription, promoters are specific sequences of _____ recognized by ______


DNA; sigma factors

Stop codons are also called?

nonsense codons

The Tat system is involved in

Gram positive bacteria

The function of DNA polymerase is to catalyze

the addition of deoxynucleotides

Transcription in eukaryotes occur in the ?

Nucleus

During DNA replication Okazaki fragments are linked together by_____ an enzyme that creates phosphodiester bonds between nicked fragments of DNA.

DNA ligase

Central dogma

DNA --> RNA --> Protein

Converting DNA code into mRNA is

Translation

Translation happens in the?

Cytoplasm

A string of Amino acids makes up

A protein

Instead of Thymine, RNA has

Uracil

Most prokaryotic genomes are?

Double stranded circular DNA

Inverted repeats are common features of transcription termination sequences in?

All three domains of life

IN nature, the predominate form of DNA is?

Supercoiled in a negative direction

Genes are found on plasmids and do impact?

Metabolism or cellular structures

DNA replication is a

Bidirectional in prokaryotes with circular chromosome

RNA acts as both?

genetic and the functional levels

RNA is capable of?

forming a secondary structure

The formation of DNA

Requires energy

DNA replication involves the synthesis of an RNA primer on?

One strand of the DNA

mRNA

Messenger RNA carries copies of instructions forthe assembly of amino acids into proteins from DNA to the rest of the cell

rRNA

Ribosomal RNA: in the cytoplasm; directs thetranslation of mRNA into proteins

tRNA

transfer RNA: in thecellular cytoplasm and is involved in protein synthesis

What is the main enzyme that transcribes DNA into RNA

RNA polymerase

How does RNA polymerase know where to start and stop?

RNA polymerase will only bindto DNA in an area called the promoter region, which have specific basesequences. There are also non-coding regions of DNA called terminationsequences which tell RNA polymerase to stop transcribing.

Describein detail how RNA is edited prior to leaving the nucleus (only in Eukaryotes).

The pieces removed are called introns(intervening sequences) and are cut out why the RNA is still inside thenucleus. The remaining portions, called exons, or expressed sequences, arespliced back together to form the final mRNA.

Whatmonomers make up polypeptide chains (proteins)?

Proteins

The language of mRNA instructions is called the genetic code. This code is a chain of nucleotides. This code is read by ribosomes in 3 letter sequences called?

codons

What is the first amino acid laid down in every protein made in eukaryotes?

Methionine

Translation begins when the mRNA molecule in the cytoplasm attaches to a ribosome. This is called?

initiation

The three bases on the tRNA molecule, called the _________, are complimentary to one of the mRNA codons

anticodons

Like an assembly line worker who attacks one part to another, the ribosome forms a peptide bond between the first and second amnio acid called what?

Elongation

When the ribosome reaches a stop codon, itreleases the newly formed polypeptide and the mRNA molecule, completing theprocess of translation. This is called what?

termination

The function unit of genetic information is the....

gene

the function od DNA gyrate is....

introduces supercoil into DNA

Eukaryotic genes have coding and noncoding regions called?

Exons= coding sequence




Introns= intervening sequence

3 enzymes involved in replication are....

Helicase


Primase


DNA Polymerase

The site of initiation of transcription

promoter

Occur when proteins are exposed to extremes of heat, pH, or certain chemicals

Denaturation

Occur when ribosomes reaches a stop codon

Termination RNA--> proteins

Three informational macromolecules in cell

DNA, RNA, Proteins

The portion of an enzyme to which substrates bind is referred to as the....

Active site


S------ E----- P

Amino acids are linked by _____ bonds to form a -______

peptide; polypeptide

The central dogma of molecular biology

DNA -trancription--> RNA -- translation--> proteins

A bacterium we have worked with in the lab

proteus vulgans




Pseudomonas aenginosa

Aseptic technique refers to....

series of practices to avoid contamination

The structure and function of a protein are determined by its _____ sequence.

amino acid

The function of RNA polymerase is to...

Copy DNA sequence into RNA sequence in`

The embed-Myerhof-parnas pathway is another name for

Glycolysis

3 types of RNA involved in transcription

tRNA, rRNA, mRNA

The function of the DNA polymerase is to catalyze

to make DNA from nucleotides

genetic elements that replicate independently of the host chromosome are called

Plasmids

Bacteria that has 52 distinct ribosomal proteins

Escherichia Coli