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60 Cards in this Set
- Front
- Back
The functional unit of genetic information |
Gene |
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Super coming is important for DNA structure because? |
It makes DNA smaller so that it can fit inside the cell |
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Plasmids |
-small circular DNA found in bacteria and eukaryotes. - Different sizes - aren't essential to bacteria but they help with survival |
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Chromosomes |
- are in the nucleus -only seen during interphase - Chromosomes are two strands of DNA - covered in proteins - Carries genetic information |
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DNA replication always proceeds in only one direction because the ________ of the incoming nucleotide is attached to the free _____ of the growing DNA strand. |
5' and 3' |
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The template for RNA polymerase is? |
a strand of DNA |
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The new RNA chain is _____ to the template. |
Anti parallel
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IS the process of transcription, promoters are specific sequences of _____ recognized by ______ |
DNA; sigma factors |
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Stop codons are also called? |
nonsense codons |
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The Tat system is involved in |
Gram positive bacteria |
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The function of DNA polymerase is to catalyze |
the addition of deoxynucleotides |
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Transcription in eukaryotes occur in the ? |
Nucleus |
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During DNA replication Okazaki fragments are linked together by_____ an enzyme that creates phosphodiester bonds between nicked fragments of DNA. |
DNA ligase |
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Central dogma |
DNA --> RNA --> Protein |
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Converting DNA code into mRNA is |
Translation |
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Translation happens in the? |
Cytoplasm |
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A string of Amino acids makes up |
A protein |
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Instead of Thymine, RNA has |
Uracil |
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Most prokaryotic genomes are? |
Double stranded circular DNA |
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Inverted repeats are common features of transcription termination sequences in? |
All three domains of life |
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IN nature, the predominate form of DNA is? |
Supercoiled in a negative direction |
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Genes are found on plasmids and do impact? |
Metabolism or cellular structures |
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DNA replication is a |
Bidirectional in prokaryotes with circular chromosome |
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RNA acts as both? |
genetic and the functional levels |
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RNA is capable of? |
forming a secondary structure |
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The formation of DNA |
Requires energy |
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DNA replication involves the synthesis of an RNA primer on? |
One strand of the DNA |
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mRNA |
Messenger RNA carries copies of instructions forthe assembly of amino acids into proteins from DNA to the rest of the cell |
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rRNA |
Ribosomal RNA: in the cytoplasm; directs thetranslation of mRNA into proteins |
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tRNA |
transfer RNA: in thecellular cytoplasm and is involved in protein synthesis |
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What is the main enzyme that transcribes DNA into RNA |
RNA polymerase |
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How does RNA polymerase know where to start and stop? |
RNA polymerase will only bindto DNA in an area called the promoter region, which have specific basesequences. There are also non-coding regions of DNA called terminationsequences which tell RNA polymerase to stop transcribing. |
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Describein detail how RNA is edited prior to leaving the nucleus (only in Eukaryotes). |
The pieces removed are called introns(intervening sequences) and are cut out why the RNA is still inside thenucleus. The remaining portions, called exons, or expressed sequences, arespliced back together to form the final mRNA. |
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Whatmonomers make up polypeptide chains (proteins)? |
Proteins |
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The language of mRNA instructions is called the genetic code. This code is a chain of nucleotides. This code is read by ribosomes in 3 letter sequences called? |
codons |
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What is the first amino acid laid down in every protein made in eukaryotes? |
Methionine |
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Translation begins when the mRNA molecule in the cytoplasm attaches to a ribosome. This is called? |
initiation |
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The three bases on the tRNA molecule, called the _________, are complimentary to one of the mRNA codons |
anticodons |
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Like an assembly line worker who attacks one part to another, the ribosome forms a peptide bond between the first and second amnio acid called what? |
Elongation |
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When the ribosome reaches a stop codon, itreleases the newly formed polypeptide and the mRNA molecule, completing theprocess of translation. This is called what? |
termination |
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The function unit of genetic information is the.... |
gene |
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the function od DNA gyrate is.... |
introduces supercoil into DNA |
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Eukaryotic genes have coding and noncoding regions called? |
Exons= coding sequence Introns= intervening sequence |
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3 enzymes involved in replication are.... |
Helicase Primase DNA Polymerase |
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The site of initiation of transcription |
promoter |
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Occur when proteins are exposed to extremes of heat, pH, or certain chemicals |
Denaturation |
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Occur when ribosomes reaches a stop codon |
Termination RNA--> proteins |
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Three informational macromolecules in cell |
DNA, RNA, Proteins |
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The portion of an enzyme to which substrates bind is referred to as the.... |
Active site S------ E----- P |
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Amino acids are linked by _____ bonds to form a -______ |
peptide; polypeptide |
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The central dogma of molecular biology |
DNA -trancription--> RNA -- translation--> proteins |
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A bacterium we have worked with in the lab |
proteus vulgans Pseudomonas aenginosa |
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Aseptic technique refers to.... |
series of practices to avoid contamination |
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The structure and function of a protein are determined by its _____ sequence. |
amino acid |
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The function of RNA polymerase is to... |
Copy DNA sequence into RNA sequence in` |
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The embed-Myerhof-parnas pathway is another name for |
Glycolysis |
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3 types of RNA involved in transcription |
tRNA, rRNA, mRNA
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The function of the DNA polymerase is to catalyze |
to make DNA from nucleotides |
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genetic elements that replicate independently of the host chromosome are called |
Plasmids |
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Bacteria that has 52 distinct ribosomal proteins |
Escherichia Coli |