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39 Cards in this Set
- Front
- Back
Generation time
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Time it takes for bacteria to grow in optimal conditions
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Generation time
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The counting of the cells
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Plate Count
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directly couning on the plate
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Optical Density
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Counting based on how clear the fluid is
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Direct Microscope count
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Counting on the microscope
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Growth Phases
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Lag- Inital statge..Lots of division
Log- Acitivity during growth Stationary- Producing at the same rate they are dying Death- Nutrients decline, reproduce slower than they die. |
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Thermophiles
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Like increased temo 45-80c.
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mesophiles
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Human body temps- 20-50c
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Psychrophiles
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Cold temps- burried under ice
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Obligate Aerobe
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Must have O2
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Microaerophile
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Likes just a little less O2 in the environment
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Obligate Anaerobe
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Does not like any O2 in the environement
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Aerotolerant
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Does not mind O2 in the environment
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facultative Anaerobe
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Doesnt mind O2 in the environment but perfers O2.
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Capnophiles
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Grows better with with more than normal Co2 in the environment. 5% more.
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Why is O2 dangerous?
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Due to SOD turning Co2 into H2O2. A catalase then turns it into H20 + O2 to make it safe again.
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Acidophiles
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Like PH level between 0-5
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Neutrophiles
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Like PH level between 5-8
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Alkalinophiles
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Like PH level between 8-12
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Activity of H2O (AW0
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Foods with high water concentrate will spoil faster due to increase bacteria growth
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UV Light
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required by some organisms to live.
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UV safe or dangerous? WHY?
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Dangerous due to the fact that it kills DNA
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What is the hardest to kill?
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Endospores
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Basic Growth factors?
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CHNOPS, K, Mg, Fe, Ca, Mn, Zn, Cu
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Activation energy
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The amount of energy needed to bring molecules together to initate reactions.
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Concentration
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the # of enzymes per reaction impacts the time.
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PH Concentration
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Bell Cure.... the abily to have a range with the PH
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temp Concentration
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Curve with a Drop due to proteins being denatured at a certain temp
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Allosteric regulation
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how it physically affects the enzymes ability to work.
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Anabolism
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Building of new molecules
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catabolism
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Breaking down of molecules
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Catabolism 3 big stages
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Glycolysis, Kreb Cycle, and ETC
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Substrate level phosphorylation
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ATP is releasing a (P) and goes directly to the enzyme, enzyme can add that (P) to another molecule
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carrier Molecules
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NAD & FAD
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Oxidation
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Loss of electrons, gain of O2
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reduction
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Storing energy gain of electrons
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Use of NAD & FAD
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to break up glucose molecules for energy
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Oxidation formula of NAD & FAD
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NADH & FADH2
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Where does cellular respiration happen?
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Glycolysis happens in Cytoplasm
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