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43 Cards in this Set
- Front
- Back
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Acid Fast Test |
Tests cell wall to ID ac-fast; Mycobacterium |
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Acid Fast Positive |
bright red/pink |
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Bacitracin Test |
Group A hemolytic strep |
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Bacitracin Positive |
Zone of Inhibition |
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Bile Esculin Test |
Group D Strep |
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Bile Esculin Positive |
Black |
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Catalase Test |
Catalase Enzyme (oxygen) |
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Catalase Positive |
Bubbles |
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Citrate Test |
Citrate Metabolism |
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Citrate Positive |
Blue |
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Citrate Negative |
Green |
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Coagulase Test |
S. Aureus (gram positive) |
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Coagulase Positive |
Clotting |
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EMB Agar Test |
Detects lactose fermentation |
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EMB Agar Positive |
purple/black; blue/black; metallic green colonies |
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EMB Agar Negative |
Colorless or Pale Pink Colonies |
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Endospore Stain Test |
Visibility of stained endospores |
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Endospore Stain Positive |
Bacillus or Clostridium |
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Gram Stain Positive |
Purple |
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Gram Stain Negative |
Red/Pink |
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Hemolytic Reaction Test |
Alpha, Beta, gamma hemolytic |
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Hemolytic Alpha |
Green Zone |
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Hemolytic Beta |
Clear Zone |
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Hemolytic Gamma |
No hemolysis so no change |
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MacConkey's Agar Test |
Detects Lactose Fermentation |
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MacConkey's Agar Positive |
Pink Colonies |
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MacConkey's Agar Negative |
Colorless Colonies |
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Mannitol Salt Agar Test |
tests for gram positive Staph |
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Mannitol Salt Agar Positive |
Yellow (fermentation) |
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Methyl Red Test |
Tests for quantity of organic acid production from glucose fermentation |
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Methyl Red Test Positive |
Orange/Red; high concentration |
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Methyl Red Test Negative |
Yellow; low concentration |
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Morphology Test |
Shapes of individual bacteria |
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Morphology shapes |
rods/cocci/vibrio/spirillum/Spirochete |
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Oxygen Test |
Strict Aerobe/Microaerophillic/Facultative Anaerobe/Aerotolerant/Strict Anaerobe |
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Oxygen Test Requirements |
Tell by growth from either Thioglycollate brothes, Melted YTA tubes, or Brewer Jars |
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Salt Broth Positive |
Growth is present |
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Salt Broth Negative |
Growth is not present |
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SIM Hydrogen Sulfide Positive |
Black |
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SIM Indole (tryptophan by-product) Positive |
Cherry Red Top Layer |
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SIM Motility Positive |
Cloudy/Hazy |
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Urease Test |
Detects urease enzyme |
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Urease positive |
Pink |
