Micro Lab Test 2

Front 1

What is the purpose of the Urease Test?

Back 1

To differeniate organisms based on their ability to hydrolyze urea with the enzyme urease.

Front 2

How is Urea produced?

Back 2

It's a product of decarboxylation of certain amino acids. It can be hydrolyzed to ammonia and carbon dioxide by bacteria containing the enzyme urease.

Front 3

Why was the Urea agar formulated?

Back 3

To differeniate rapid urease + organisms from the slower urease + and urease - bacteria.

Front 4

What are the 2 differences between urea agar and urea broth?

Back 4

Urea broth only has one nutrient source, trace of yeast extract. It also contains buffers strong enough to inhibit alkalinzation of the medium but the rapid urease + organisms.

Front 5

what is the purpose of the Gelatinase test?

Back 5

to derernmine the ability of a microbe to produce gelatinases.

Front 6

What are gelatinases?

Back 6

Protein dervived from collagen, Gelatinases comprise a family of extracellular enzymes produced and secreted by some microorganisms to hydrolyze gelatin.

Front 7

What does SIM mean?

Back 7

Sulfur-Indole-Motility

Front 8

What kind of medium is SIM?

Back 8

Combination Differential

Front 9

What is a stab inoculation and why is it used?

Back 9

When you stab the agar with the loop and it is used to produce anaerobic/motility microbes for transfer to another medium. It's used for differential mediums.

Front 10

What is Enviromental Microbiology?

Back 10

The study, utilization, and control of microorganisms living in marine, freshwater, or terrestrial habitats.

Front 11

What is the purpose of the membrane filter technique?

Back 11

commonly used in combination with other tests to identify the presence of fecal coliforms.

Front 12

What is the microbial soil count for?

Back 12

To isolate and demonstrate the variety if populations residing in soil.

Front 13

What is meant by food microbiology?

Back 13

Study, utilization, and control of microorganisms in food such as yogurt, wine, beer, sauerkraut, buttermilk, etc.

Front 14

What is the purpose of the methylene blue reductase test?

Back 14

This test is helpful in differentiating the enterococci from other streptococci. It also test for the presence if coliforms in raw milk.

Front 15

What is the purpose of the Snyder Test?

Back 15

to measure dental carries susceptibility caused by primarily by lactobacilli and oral streptococci.

Front 16

What is the color of a + synder test?

Back 16

yellow

Front 17

What is antimicrobial testing?

Back 17

Used to measure the effectiveness if antibiotics and toehr chemotherapeutic agents on pathogenic microorganisms.

Front 18

What are antibiotics?

Back 18

natural antimicrobial agents produced by microorganisms

Front 19

What is the Kirby-Bauer Test used for?

Back 19

for measuring of the effectiveness of antimicrobics against pathogenic microorganisms.

Front 20

What is the zone if inhibition?

Back 20

The sensitivity of the bacteria to the specific antimicrobial agent and the point at which the chemicals MIC is reached.

Front 21

What is MIC?

Back 21

Minimum inhibitory concentration

Front 22

What is a synergistic effect?

Back 22

A clear area between the disks extending beyond the perimeters of the otherwise circular zones.

Front 23

What are pyrimidine dimers?

Back 23

Cytosine-cytosine, cytosine-thymine, thymine-thymine

Front 24

What is photoreactiviation?

Back 24

Light repair in which the repair enzyme, DNA photolyase, is activated by light and simply monomerizes the dimer by reversing the original reaction.