Micro Lab Quiz 3

Front 1

what is the difference between moist and dry heat?

Back 1

moist heat uses water and dry heat does not

Front 2

what are 3 types of moist heat?

Back 2

boiling, autoclaving, pasteurizing

Front 3

what are 2 types of dry heat?

Back 3

ovens and incinerators

Front 4

what is thermal death time?

Back 4

length of time required to kill all bacteria in a sample

Front 5

what is the difference between antiseptics and disinfectants?

Back 5

antiseptics are used on living tissue and disinfectants are used on inanimate objects

Front 6

what is a zone of inhibition?

Back 6

area around an antimicrobial disk with no growth

Front 7

what does it mean if there is no zone of inhibition?

Back 7

agent is ineffective

Front 8

what type of agar is used for the antimicrobial disk diffusion assay?

Back 8

Mueller Hinton

Front 9

what is the mode of action for tetracycline?

Back 9

inhibit protein synthesis

Front 10

what is the mode of action for penicillin?

Back 10

inhibit cell wall synthesis

Front 11

what is the mode of action for vancomycin?

Back 11

inhibit cell wall synthesis

Front 12

what is the mode of action for erythromycin?

Back 12

inhibit protein synthesis

Front 13

what is the mode of action for rifampin?

Back 13

inhibit nucleic acid synthesis

Front 14

why do we measure the zone of inhibition?

Back 14

to test whether the agent is resistant, intermediate, or susceptible

Front 15

how do you know which antibiotic is most effective?

Back 15

the largest zone size

Front 16

what is a starter culture?

Back 16

bacteria used to initiate souring

Front 17

what is the purpose of the milk in the yogurt experiment?

Back 17

provides lactose to make lactic acid

Front 18

what is fermented in the yogurt experiment?

Back 18

bacteria

Front 19

what bacteria are involved in the yogurt experiment?

Back 19

lactobacillus thermophilus and streptococcus bulgaricus

Front 20

what is a catalase test?

Back 20

hydrogen peroxide is placed into the bacteria growth

Front 21

what type of agar is used for the throat swab test?

Back 21

blood agar

Front 22

what are the 3 steps of a disk diffusion assay?

Back 22

uniform inoculation

disks placed on surface

incubation causes diffusion of disks

Front 23

what is bacteria fermentation?

Back 23

carbohydrates are broken down for energy in the absence of oxygen

Front 24

what is the streak method?

Back 24

bacteria falls off with each streak and colonies are created

Front 25

what is the spread method?

Back 25

bacteria is spread evenly and easily counted

Front 26

what is the pour method?

Back 26

agar and bacteria are poured into Petri dish to minimize contamination but risks aerobically growing bacteria

Front 27

what is selective agar

Back 27

prevents growth of unwanted bacteria without inhibiting desired bacteria

Front 28

what is differential agar?

Back 28

used to identify bacteria by how they grow in agar

Front 29

what is the selective part of MSA?

Back 29

salt

Front 30

what is the differential part of MSA?

Back 30

mannitol

Front 31

what is the selective part of EMB?

Back 31

added dyes

Front 32

what is the differential part of EMB?

Back 32

added sugar

Front 33

what is normal flora?

Back 33

salt-tolerating, drying-resistant bacteria that is always on skin

Front 34

what is the difference between s.aureus and s.epidermidis?

Back 34

SA ferments mannitol and turns it yellow

SE tolerates salt and grows on MSA

Front 35

what is alpha hemolysis?

Back 35

partial rbc and hemoglobin destruction

Front 36

what is beta hemolysis?

Back 36

complete destruction of rbc

Front 37

what is gamma hemolysis?

Back 37

no rbc destruction