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14 Cards in this Set

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Facultative anaerobe

Does not require oxygen can grow with or without it. Able to detoxify toxic byproducts of oxygen



Growth throughout test tube



Example: E.Coli

Microaerophile

Does not grow an atmospheric oxygen but requires a small amount for growth. Detoxifies toxic byproducts of oxygen



Grows in the middle of the tube



Example: micrococcus luteus

Anaerobe

Lacks the enzyme for using oxygen can't live in oxygen. Not able to detoxify toxic byproducts of oxygen



Gross at the bottom of tube



Example; clostridium

Anaerobic jar

Remove O2 increase CO2 concentration



BD GasPak contain inorganic carbonate



Turns blue in presence of O2 and colorless in presence of CO2

Fastidious bacteria

Excessively particular, critical, or demanding

Fluid thioglycollate medium

Used for aerobic, anaerobic, fastidious and non fastidious organisms


Enterics

Rod-shaped, Gram-negative, gut bacteria. Most occur normally or pathogenically in intestines

Enteric plates

Selective media for isolation of gram-negative bacteria



Example: Eosin Methylene Blue Agar

Coliforms non-pathogenic bacteria

Rod-shaped, Gram-negative, non-spore forming bacteria which can ferment lactose with the production of acid and gas at 35-37C



Example, E. Coli, Enterobacter aerogenes

Opportunistic pathogens

Proteus mirabilis



Only when immune system is weak

True pathogens

Salmonella spp, and Shigella spp



Pathogens that causes disease in virtually any susceptible host

Eosin methylene blue agar(EMB)

Used in isolation of gram-negative bacteria



E-coli-green metallic Sheen, strong lactose or sucrose fermenter



Enterobacter-pink, slow lactose fermenter and little acid producer

Hektoen enteric agar (HE or HEK)

Uses bile salts to inhibit growth of gram+ bacteria



Shigella, salmonella and proteus (bluish green colonies, sugar non fermenter)



Salmonella and Proteus have colonies with black precipitate (reduce sulfur to H2S)



Shigella no black precipitate



E-coli, enterobacter (yellow to pink colonies)

Enterotube

Has 12 compartments and 15 tests