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33 Cards in this Set
- Front
- Back
What is the 1st biosafety level?
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BSL-1: Do not typically cause diseases in healthy people. There is minimal threat to lab and environment. No special containment equipment required
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What is the 2nd biosafety level?
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Commonly encountered in the community. Present moderate environmental/health hazards. Associated with human diseases of varying severity. Class I or II BSC. Injury, ingestion, etc.
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What are two ways to adjust the light on a microscope?
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1. The iris diaphram
2. Light Intensity knob (voltage regulator) |
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What are the objectives on a microscope?
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x4
x10 x40 x100 |
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What is a hanging drop preparation?
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A way to prep the slide onto a special depression microscrope slide
Allows longer observation of the object because it does not dry out as quickly |
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What were the two agar plates that we used?
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SDA: encourages growth for media
TSA: for bacteria |
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How many micrometers are between each line on the stage micrometer?
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10
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How many micrometers are between the lines on the ocular micrometer?
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We don't know. This is why we calibrate...to find out this distance
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What is the formula to calibrate the ocular micrometer?
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ocular lines (unknown) = stage lines X 10
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What were the calibration results?
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4X: 12.5 um
10X: 5 um 40X: 1.25 um 100X: .05 um |
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A major group of amoeboid protozoa?
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Amoebozoa
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What is a characteristic of amoebozoas?
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amobeoid pseudopodia (
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Are protozoans heterotrophic?
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yes (they are also unicellular eukaryotics)
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entamoeba histolytica (trophozoite)?
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Protozoa: SG Amoebozoa: Pseudopodia: Areas with poor sanitation. fecal/oral
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What can withstand the acidic environment of teh stomach?
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Cysts
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Amoeba Proteus?
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Protozoa: SG Amoebozoa: Pseudopodia: Fresh water environments and feeds on other protozoans
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Girardia lamblia?
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Protozoa: SG Excavata: Flagella: Fecal contaminated water. Pets often have this. Flagellate protozoan
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Trypanosoma Gambiense?
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Protozoa: SG Excavata: Flagella: Produces African Sleeping Sickness. Intermediate (vector) host - the tsetse fly.
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Trichomonas vaginalis?
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Protozoa: SG Excavata: Flagella: Effects both men and women. Has flagella. Usually through sexual intercourse
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Paramecium?
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Protozoa: SG Chromalveolata: cilia: Usually found in fresh water
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Plasmodium vivax?
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Protozoa: SG Chromalveolata: cilia: no locomotion. Parasite: causes malaria. Obtained thru a mosquito
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Cytosporidium?
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Protozoa: SG Chromalveolata: cilia. no locomotion. most commonly isolated from feces from HIV patients
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What is the name for parasites that we studies?
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Helminths
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Parasites are all from what super group?
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Opishthokonata
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What are characteristics of the phylum: Playthyhelminthes?
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Unsegmented flatworms
More primitive Incomplete digestive track Hermaphroditic No body cavity |
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What are characteristics of the phylum: Nematoda?
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Unsegmented
Complete digestive track Separate sexes Unsegmented round worms pseudocoelon (body cavity) |
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Class Trematoda (flutes):
aka Liver Flukes Name the two species |
Phylum Plathyhelminthes
Clornorchis sinensis Schistosoma |
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Class Cestoda (tapeworms)
Name the species |
Phylum Plathyhelminthes
Taenia Solium |
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Name the 5 species that fall under the Nematoda phylum
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Ascaris lumbricoides
Necator americanus Enterobius vermicularis Wuchereria bancrofti Trichonella spiralis Any Nice Elephants Wondering Trinidad? |
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Describe Clonorchis sinensis
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Chinese liver fluke
Contaminated fish |
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Describe Schistosoma
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Blood fluke
This one has separate sexes (only exception) |
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Describe Taenia solium
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Pork Tapeworm
Causes epilepsi Undercooked meat Causes cysticercosis Scolex (head) Proglotids (sections) Gravid proglotids are released into the feces |
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Describe Enterobius vermicularis
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Pin worm
Most common in the US / children oral/fecal lays eggs on anus at night |