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18 Cards in this Set
- Front
- Back
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Phenol Red Glucose Broth w/Durham tube
Phenol Red Glucose(Acid/Gas) (Test Tube #1) |
Results:
Positive - Yellow/Bubble Negative - Red/NO Bubble |
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Mixed Acid Fermentation
MR-VP Broth MR Test (Test Tube #4) |
ADD 4 drops of Methyl Red
Results: Positive - Red Negative - Yellow |
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2, 3-Butanediol Fermentation
MR-VP Broth VP Test (Test Tube #5) |
ADD 4 drops of Barritt’s reagent
VORTEX for 10-15 seconds Then let the tube sit for about 30 minutes Results: Positive - Red Negative - Yellow |
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Catalase Production
TSA Slant Catalase Test (Test Tube #6) |
ADD 2 drops of hydrogen peroxide to the inoculated TSA slant.
Results: NO Bubbles - Negative Bubbles - Positive |
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Urea Hydrolysis (Broth)
Urease Test (Test Tube #7) |
Results:
Positive - Bright pink Negative - Yellow |
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Tryptophan Hydrolysis (Broth)
Indole Test (Test Tube #8) |
ADD 12 drops of Kovac’s reagent.
Results: Positive - Red Band Negative - Yellow Band |
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Citrate Utilization
Simmons Citrate Agar Slant Citrate Test (Test Tube #9) |
Results:
Positive - Prussian Blue Negative - Green |
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Nitrate Production
Nitrate Broth Nitrate Test (Test Tube #10) |
ADD 3 drops of reagent A
THEN add 3 drops of reagent B Results: Positive - DEEP red/orange Negative - Yellow |
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Motility (SIM) Deep
(Test Tube #11) |
Results:
Non-motile - grow ONLY along the line of inoculation(NEG) Motile - organisms SPREAD OUT from the line of inoculation and may even grow throughout the medium (POSITIVE) |
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Triple Sugar Iron (TSI Slant)
Sugar Fermentation (SLANT) (Test Tube #12) |
Results:
Positive - Yellow Negative - Red |
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Triple Sugar Iron (TSI Slant)
H2S Production(Hydrogen Sulfide) (Test Tube #12) |
Results:
Positive - BLACK Negative - Red |
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Starch Hydrolysis
Starch Agar Plate |
Pour enough Grams’ iodine over the surface of the plate to cover surface.
Results: Positive - Clear Zone along streak Negative - Dark/Black |
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Triple Sugar Iron (TSI Slant)
Sugar Fermentation(BUTT) (Test Tube #12) |
Results:
Positive - Yellow Negative - Red |
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Triple Sugar Iron (TSI Slant)
CO2 Production (GAS) (Test Tube #12) |
Results:
Positive - Bubble Negative - NO Bubble |
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Phenol Red Lactose Broth
Phenol Red Lactose(Acid) (Test Tube #2) |
Results:
Positive - Yellow Negative - Red |
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Casein Hydrolysis
Skim Milk Agar |
Results:
Positive - Clear Zone along streak Negative - No clear area |
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Phenol Red Mannitol Broth
Phenol Red Mannitol(Acid) (Test Tube #3) |
Results:
Positive - Positive Negative - Red |
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Gram Stain
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1. Obtain a clean slide
2. Apply 2 loopfuls of WATER to slide 3. Aseptically remove a colony from your TSA plate culture and PLACE in water 4. AIR DRY 5. HEAT FIX 6. Perform Gram Stain (C - 1 MIN, I - 1 MIN, E - 3-5 SEC, S - 1 MIN) 7. Gram REACTION: Positive - Purple Negative - Pink 8. Cell Shape/Cell Arrangement |
