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22 Cards in this Set
- Front
- Back
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Mycobacteria basics |
-Acid Fast
-High lipid content of cell wall, Impermiable to Gram stain
-8 weeks incubation in 5-10% CO2 |
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Mycobacteria Staining |
-Uses AFB stain and the Ziehl-Neelsen method
-Primary Stain: Carbol Fuschin
-Counter Stain: Methylene blue adn Malachite Green
-heat t0 65-70c while staining
-AFB stains as pink beaded chains, every thing else will be blue/green
-Kniyoun cold method uses a higher concentration of carbol fuschin |
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Specimine Isolation Procedure |
Decontamination and digestion of Mucos is done by add NaOH, and NALC. It is then centrifuged so that the asparate can be collected |
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Lownstein Jenny Slant |
Egg based, non selective media containing glycerol, lipids, and malachite green |
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7H10/11 Media |
Nonselective media containing Salt, Vitamins, Glycerol and Malachite Green |
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Petragani Agar |
Eggs, Whole milk, potato, glycerol, and malachite green |
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What are Rapid growing Mycobacteria? |
Grow in less than 7 days
M. fortuitium
M. chelonae |
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Scotochromogens |
Pigmented when grown in the light or dark
M. gordonae
M. scrofulaceum |
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Photocrhomogen |
Pigmented when grown in the light.
M. kansaii
M. marinum
M. simae |
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Nitrate reduction |
M. tuberculosis
M. kansasii
M. fortuitium |
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Niacin |
Nicain (Nicotinic acid) Forms as the organism grows on the agar
Yellow = +
M. tuberculosis |
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Arylsulfatase |
Looks for arylsulfatase production by the microrganism, it's prescences causes the release of a detecable phenol.
M. fortuitium |
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Heat Stable Catalase test |
Looks to see if the catalase the organism produces is heat stable (68c)
INH-resistant M. tuburculosis is positive
Most M. tb strains are negative, along with M. marinum
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Tellurite |
Looks for an organisms ability to reduce pottasium tellurite to a metalic black substance
+++ M. avium, intracellulare, fortuitum, chelonei |
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Tween Hydrolysis |
Looks for an organism ability to produce a lipase that can hydrolise the detergent tween 80 into oleic acid and sorbitol
+++ kansasii, marinum, gordonae, flavescens, and fortuitum. |
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Semi-Quant catalase |
Compares the amount of catalse produced by organisms after they've grown on a Lownstein- Jensen agar
+++ = over 45 mm |
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M. kansaii |
Photochromogen
Nitrate +
Semi-Qaunt +
68c-cat + |
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M. marinum |
Photochromogen
30c+
Nitrate -
Semi-Quant -
68c-cat - |
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M. gordonae |
Scotochromogen
Smooth Colony
Tween +
Urease - |
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M. scrofulaceum |
Scotocrhomogen
Smooth colony
Tween -
Urease + |
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M. fortuitium |
Rapid Growth
Nonchromogen
smooth colonies
arysulfatase +
Nitrate + |
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M. chelonae |
Rapid Growth
Nonchromogen
smooth colonies
arysulfatase + Nitrate - |
