Micro Lab

Front 1

Organism isolated from an abscess in the interdigital space of a dog. It is coagulase + and shows DZH.

Back 1

Staphylococcus pseudintermedius

Front 2

What are symptoms caused by Staph pseudintermedius?

Back 2

pyodermas (sores between toes), mastitis in dogs and cattle

Front 3

______ Staphylococcus species that was previously confused with S.intermedius because the two organisms are biochemically very similar

Back 3

Staph pseudintermedius

Front 4

Organism isolated from the urinary tract of a 4-year-old dairy cow that had dark, red wine-colored urine. It was urease positive.

Back 4

Corynebacterium renale

Front 5

___________________ is the cause of ulcerative posthititis (swelling of prepuce) of sheep and goats.

Back 5

Corynebacterium renale

Front 6

Which G+ rod is the only consistently urease positive (and strongly)?

Back 6

Corynebacterium renale. Rhodococcus equi is variable.

Front 7

This organism was isolated from ab abscessed lymph node in a dairy goat. It was a G+ rod, dry colonies, urease (+).

Back 7

Corynebacterium pseudotuberculosis

Front 8

__________________ is the most common cause of abscesses in smallruminants.

Back 8

Corynebacterium pseudotuberculosis

Front 9

Methicillin resistance is seen in isolates of these colonies:

Back 9

Staph pseudintermedius

Staph aureus

Front 10

This was isolated form the lungs and joints of a 5-month-old foal that was severely lame and had chronic pneumonia. The colonies were G+ rods, medium sized, and heavily mucoid.

Back 10

Rhodoccocus equi

Front 11

What symptoms would you expect from a horse with Rhodoccocus equi?

Back 11

pneumonia

Front 12

This organism was isolated from a pig that was severely lame and then died. It has tiny cultures and a black Kliger's tube (H2S production).

Back 12

Erysipelothrix rhusiopathiae

Front 13

This organism was small and a gram positive coccus. It was isolated from a pig that was down and paddling.

Back 13

Strep suis

Front 14

This organism causes CNS infections, arthritis, septicemia in pigs, rarely (but sometimes) humans.

Back 14

Strep suis

Front 15

This organism is CAMP positive, shows some hemolysis and is a cause of mastitis in dairy cows.

Back 15

Strep agalactiae

Front 16

This organism is CAMP - and has a green alpha hemolytic pattern. It is a cause of mastitis in dairy cows.

Back 16

Strep dysgalactiae

Front 17

This organism is an non-hemolytic, G+ coccus that is coagulase (-), small, and white. It can cause mastitis in dairy cows.

Back 17

Staph epidermidis

Front 18

This organism might show DZH, is yellow, and coagulase (+).

Back 18

Staph aureus

Front 19

This organism was isolated from an abscessed lymph node on the neck of a horse. The horse had been off feed for a week and had a moderately high fever for 5 days.

Back 19

Streptococcus equi

Front 20

The organism has 84 different M-proteins and would therefore be unlikely to generate an IgA response.

Back 20

Strep pyogenes

Front 21

This organism can easily be transmitted to fomites (objects or materials that are likely to carry infection, such as clothes, utensils, and furniture.)

Back 21

Strep equi

Front 22

This organism was isolated from the blood of a dead angus cow. They were large G+ rods, susceptible to penicillin, and it looked wet on the plate.

Back 22

Bacillus anthracis

Front 23

This organism was isolated from the skin wound on the leg of a 2-year-old horse. It was a beta hemolytic strep.

Back 23

Strep zooepidemicus

Front 24

This organism was isolated from ice cream that made everyone sick, including two abortions. The organisms were small G+ rods, alpha hemolytic, and at 24 hrs had small, white colonies.

Back 24

Listeria monocytogenes

Front 25

_______________ is an organism that commonly grows on plants andin soil and intestinal tracts of herbivores and causes neurologic disease andabortions in cattle, sheep and others.

Back 25

Listeria monocytogenes

Front 26

____________________ an organism that produces a toxin which breaksdown the phagosomal membrane which allows the organism to grow in the cytosol.

Back 26

Listeria monocytogenes

Front 27

__________________ is acause of unilateral facial paralysis, meningoencephalitis, blindness andabortions in ruminants.

Back 27

Listeria monocytogenes

Front 28

This organism was isolated form a sample of rice implicated in an outbreak of food poisoning. It is a large, spreading colony that has a ground glass appearance. You note G+ rods and beta hemolysis.

Back 28

Bacillus cereus

Front 29

This organism was isolated from a large abscess on the hock of a dairy cow. It is a very small G+ rod (looks like a cocci). Growth of these colonies at 24 hours is quite small. It cleared a casein agar.

Back 29

Trueperella pyogenes

Front 30

For which organism is the casein agar test used?

Back 30

Trueperella pyogenes will turn it clear (+)

Front 31

Which are the alpha hemolytic G+ cocci?

Back 31

S. dysgalactiae, Strep uberis, Strep suis, Enterococcus

Front 32

This is a common cause of sore throats in humans, along with wounds and septicemia

Back 32

Strep pyogenes

Front 33

Causes otitis externa in dogs. Small, G+ rods, yellow, mucoid.

Back 33

Corynebacterium auriscanis

Front 34

This organisms can cause septicemia, arthritis, and skin necrosis in both swine and turkeys. It is very small and shows alpha hemolysis.

Back 34

Erysipelothrix rhusiopathiae

Front 35

Which G+ rods are hemolytic?

Back 35

Listeria monocytogenes (weak beta)

Erysipelothrix rhusiopathiae (alpha)

Trueperella pyogenes (weak beta)

Note that both tiny ones are hemolytic!

Front 36

This is the only G+ rod that shows motility.

Back 36

Listeria monocytogenes

Front 37

Which organisms show DZ hemolysis? Be specific with the names. How can you confirm you have one of these?

Back 37

Firstly, DZH can only be Staph:

- Staph aureus

- Staph schleiferi

- Staph pseudintermedius

These are all coagulase positive. (NOTE: only Staph schleiferi subsp. coagulans is positive. subsp. schleiferi won't be.)

Front 38

The sodium hippurate test, when done correctly, can distinguish between these two organisms. Which will be positive?

Back 38

Strep dysgalactiae (-) and Strep uberis (+)

Front 39

What is the VP test used to determine? Which are (+)? Which are (-)?

Back 39

Distinguish between staph

(+) Staph aureus, Staph scheliferi, Steph epidermidis

(-) Staph hyicus, Staph pseudintermedius

Front 40

This organism causes greasy pig syndrome. It is a coagulase (+), G+ cocci.

Back 40

Staphyolococcus hyicus

Front 41

This genus of bacteria will form grape-like clusters under the microscope.

Back 41

Staphylococcus

Front 42

Which test is the standard of pathogenicity for G+ cocci (aka, determines which organisms are most pathogenic)?

Back 42

coagulase positive organisms are considered to be the most pathogenic

Front 43

Which organism has a "narrow zone" hemolysis?

Back 43

Trueperella pyogenes

Front 44

If an organism is raffinose positive and your results don't fit the history, what is it likely to be?

Back 44

Enterococcus

Front 45

This organism can cause tonsilitis, mastitis, and septicemia in dogs and cats. It is a G+ cocci.

Back 45

Streptococcus canis

Front 46

This G+ coccus can cause jowl abscesses in pigs.

Back 46

Strep porcinus

Front 47

These two genera are small, G+ non-spore-forming rods that can cause disease in several species.

Back 47

Listeria monocytogenes

Erysipelothrix rhusiopathiae

Front 48

Which G+ rod exhibits a CAMP-like reaction?

Back 48

Rhodococcus equi

Front 49

This large, G+ rod produces a ground glass appearance and produces a strong, sharp zone of hemolysis. It is penicillin resistant.

Back 49

Bacillus cereus

Front 50

______________ is primarily a swine pathogen that caused a relativelylarge outbreak of meningitis in farmers in China in 2005. Most of those infected had butchered dead orsick swine.

Back 50

Strep suis

Front 51

_________________ is primarily a swine pathogen that has beenrecovered lately from several cases of genito-urinary tract infections inhumans.

Back 51

Strep porcinus

Front 52

Which two organisms are commonly involved in TSS in the US?

Back 52

Straph aureus

Strep pyogenes

Front 53

_____________________ is a common cause of abscesses and uterine infectionsin cattle and is a secondary invader in pulmonary infections in a number ofanimal species.

Back 53

Trueperella pyogenes

Front 54

___________ causes ascending infections of the urinary tractof swine.

Back 54

Strep sui

Front 55

________________ is carried in the tonsils and intestine of about30% to 50% of normal swine and is also a frequent cause of septicemia in swine.

Back 55

Erysipelothrix rhusiopathiae

Front 56

_________ is currently a common cause of lower respiratorytract disease in humans.

Back 56

Strep pneumoniae and Strep pyogenes

Front 57

_________________ produces 4 different exfoliative toxins that areimportant in production of disease in young swine.

Back 57

Staph hyicus

Front 58

___________ is a common Gram-positive mastitis pathogen ofdairy cattle that is commonly found in feces and bedding

Back 58

Strep uberis

Front 59

________________ is an organism that causes peracute septicemia andtoxemia in ruminants and edematous swelling of the head and neck areas ofhorses, swine, dogs and cats.

Back 59

Bacillus anthracis

Front 60

___________ is an organism that causes pyelonephritis andsometimes metritis in periparturient swine.

Back 60

Strep suis

Front 61

____________ is the cause of large, painful abscesses in thepectoral region of horses as well as abscesses in the peritoneal cavity andelsewhere.

Back 61

Corynebacterium pseudotuberculosis var equi

Front 62

_____________ is an organism commonly found in the urethra,prepuce, and genital mucous membranes of sheep and cattle and which requirespredisposing factors in order to cause disease.

Back 62

Corynebacterium renale

Front 63

_____________ is a common swine pathogen that more commonlyproduces disease in high-health-status swine herds.

Back 63

Strep suis

Front 64

These two organisms were isolated from the reproductive tract of two different mares that failed two breed. On is mucoid and pink on MacConkey's. The other is beta hemolytic and coccoid.

Back 64

Mucoid - Klebsiella pneumoniae

Beta - Strep zooepidemicus

Front 65

Organism that is swarming and has a foul odor. It was isolated from the ear canal of a dog with chronic otitis externa.

Back 65

Proteus mirabilis or vulgaris

Front 66

This organism makes little donuts on Mac. It was isolated from a 3-day-old calf with scours.

Back 66

Escherichia coli

Front 67

This organism is G+, very dry looking, and takes 8 weeks to grow. It was isolated from a rectal biopsy of a 3-yr-old cow that was losing condition rapidly.

Back 67

Mycobacterium avium subsp paratuberculosis

Front 68

This organism is very small and G+ rod. It was isolated from the blood and liver of an 8-week-old turkey found dead.

Back 68

Erysipelothrix rhusiopathiae