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105 Cards in this Set
- Front
- Back
BA stands for
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Blood Agar
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What catergory is BA under?
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Differential
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What is the purpose of BA?
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to test for hemolytic acitvity
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What does MAC stand for?
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MacConkey Agar
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What catergory is MAC under?
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Differential and Selective
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What is the purpose of MAC?
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for isolation of Gram (-) Enterics
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What does PEA stand for?
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Phenylethyl Alchohol Agar
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What catergory is PEA?
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Selective
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What is the puropse of PEA?
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Isolation of Gram (+)
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What does MSA stand for?
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Mannitol Salt Agar
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What cateogry is MSA under?
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Seletive and differential
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What is the purpose of MSA?
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Isolate spahtyloccus species
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What does PA stand for?
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Pseudomonas Isolation Agar
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What cateogry is PA under?
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Selective
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What is the purpose of PA?
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isolation of pseudomonas species
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What is Staphylococcus epidermidis? (3)
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(1)on the skin (2)opportunistic (3) most strains produce a slime layer which may enalble them to attach to cetrain hopstial apparatuses during invasive procedures
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NAME
can be found on the skin and is opportunistic |
Staphylococcus epidermidis
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NAME
most strains produce a slime layer which may enalble them to attach to cetrain hopstial apparatuses during invasive procedures |
Staphylococcus epidermidis
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What is Stahphylococcus aureus? (2)
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(1)most common noscomial bacteria infection (2)causes food poisning, toxic shock, scalded skin syndrome, pinke boils etc
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causes food poisning, toxic shock, scalded skin syndrome, pinke boils etc
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Stahphylococcus aureus
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NAME
is the most common bacteria nosocomial infection |
Stahphylococcus aureus
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NAME
also known as GAS |
Streptococcus pyogenes
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GAS stands for
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Group A Streptococcus
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Streptococcus pyogenes are also called ( ).
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GAS
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NAME
is the most common cause of pharyngitis, but can also cause acute rhematic fever, and acute glomerulonephritis |
Streptococcus pyogenes
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What is Streptococcus pyogenes? (3)
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(1)also called GAS (2)common cause of pharyngitis (3) can also cause acute rheumatic fever, and acute glomerrulonephritis
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NAME
is the most common cause of pharyngitis |
Strepcoccus pygones
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Pharyngitis is ( ).
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Sore throat
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Sore throat can also be refered to as ( ).
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Pharyngitis
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What is Pseudomonas aeruginoa? (4)
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(1)Opportunitstic w phagocyte resistant slime layer (2) has a fruity odor (3) a blue green pigment (4) can cause eye and ear infections
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NAME
Opportunitstic w phagocyte resistant slime layer |
Pseudomonas aeruginosa
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NAME
is nosciminal and can cause eye and ear infections |
Pseudomonas aeruginosa
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NAME
has a fruity odor and a blue green pigment |
Pseudomonas aeruginosa
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What is Escherichia coli?
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(1)common resistant of digestive tract (2) produces Vitamin K and B (3)is a indicator bacterium
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What can Escherichia coli cause(3)?
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(1)food poisning (2) Montezumas revenge (3) UTI
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NAME
common resistant of digestive tract |
Escherichia coli
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NAME
produces Vitamin K and B |
Escherichia coli
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NAME
is a indicator bacterium |
Escherichia coli
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NAME
usual is the cause of UTI, food poisning, and Montezuma's revenge |
Escherichia coli
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Describe the Mircococcus gensis (3)
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(1)Gram (+) (2)aerobic (3)nonpathogenic
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What is Micrococcus Luteus?
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appears yellow in culture
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What is Micrococcus?
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appears red in culture
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NAME
is gram (+), aerobic, and nonpathogenic |
Micrococcus luteus and Micrococcus roseus
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NAME
appears yellow in culture |
Micrococcus luteus
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Are species of the genre Micrococcus pathogenic?
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No
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NAME
appears red in culture |
Micrococcus roseus
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Where is the sharps container located?
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in the sliver container at the end of each lab table
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What is a nosocomial infection?
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a hospital acquired infection
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How do you label plates and tubes?
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(1)Media (2)organism (3) Team (4)Date (5)Intials
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Why do you label at the bottom of plates?
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for safety reasonsn incase someone removes the top and it becomes lost
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What is the differ btwn Antiseptic and Disinfectant?
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Antiseptic kills all germs on living tissue and Disinfectant kills tissue on inmate objects
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NAME
kills all germs on living tissue |
Antiseptic
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NAME
is a hopstial acquried infection |
nosocomial infection
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NAME
kills tissue on intimate objects |
Disinfectant
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What is the general prepartation for tubes?
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melt before pipetting and autoclaving
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What is the general preparation for plates?
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autoclave and then pour
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For ( ). the general preparation involes melting before pipetting and autoclaving
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tubes
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For ( ), the general preparation involes melting before pipetting and autoclaving
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tubes
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NAME
Category is General (2) |
(1)TSA (2)TSB
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NAME
Category is Selective |
PA
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NAME
Category is Differential |
BA
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NAME
Category is both Selective and Differential (2) |
(1)MSA (2)MAC
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What are the main differnces btwn preparing for media for tubes and plates?
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For the tubes-melt before pipetting and autoclaving.
For the plates-autoclave and then pour |
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NAME
Category is Bichemical |
TGB
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What is the Category of TSB?
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General
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What is the category of TSA?
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General
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What is the catergory of TGB?
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Biochemical
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What are the steps in preparing a smear?
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(1)a drop of water (2)add organism (3)smear to the size of a quarter (4)air dry (5)heat fix
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Where does the contaminated Lab material go?
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in the Biohazardous Waste Disposal
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Give one difference btwn Antiseptic and Disinfictant with one ex of each
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Antispetic technique is used to keep tissue free of microbes and a ex is sterilizing medical equipment (2)Disinfectant is to clean the surface are and a ex is disinfectant used to kill microbes when there is a spill during lab
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What are the steps used to clean up a contaminated spill? (4)
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(1)put a paper towel over the spill (2)spray the paper towel w disinfectant (3) wait 20 min (4)clean the spill
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Which method of sterlization is used to destroy endospores and what type of heat sterliation is used?
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the autoclave is used at a temp of 121 C at 15 psi
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If by any means while doing pratical your glass slide breaks where then where should u place it?
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the sharps container
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Which standard method is used in micro lab for preventing and avoiding contamination of cultures and who used it first?
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(1)Joseph Lister (2) aseptic techinques such as washing your hands before surgury
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What does read the meniscus mean?
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read to the line lowest where the water appears to be
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What does Tare the balance refer to?
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making a 1 plus zero balance
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A recipe has 30 grams/1 liters, and how many grams of 160 ml do you want.
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4.8 grams
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How do you solve recipe problems?
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convert the give grams to mL then mutilpy by the other given
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A recipe has 50 grams/1 Liter, how many grams of 350 mL are needed?
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17.5 grams
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What does BA stand for?
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Blood Agar
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What does MAC stand for?
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MacConkey Agar
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What does PA stand for?
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Pseudomonas Isolation Agar
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What does PEA stand for?
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Phenylethyl Alcholol Agar
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What does MSA stand for?
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MAnnitol Salt Agar
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What does TGB stand for?
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Thioglycolate Broth
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What does TSA stand for?
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Trypitcase Soy Agar
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What does TSB stand for?
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Trypticase Soy Broth
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What is the purpose of TGB?
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growth of anaerobic microbes
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What is the purpose of TSB?
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the growth of a wide range of bacteria
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What tools do you use to incolulte the media? (3)
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(1)loop (2)needle (3) swab
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Name two ways for incolution of media
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(1)Agar slant (2)Agar plate
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What is Agar slant?
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a liquid broth placed in deep agar tubes
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What is agar plate?
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is a quadrant streak used for isolation or seperation of a mixed culture
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What is Clostridium sporogenes? (4)
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(1)anaerobic (2)needs a medium such as TGB (3) does not produce neurotoxins (4)can cause inststinal water loss and hemorrhaging by destroying instetinal epitheaial cells.
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NAME
are anaerobic and needs a medium such as TGB |
Clostridium sporogenes
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NAME
does not produce neurotoxins |
Clostridium sporogenes
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NAME
can cause intestinal water loss and hemorrhaging by destroying inestinal epitheal cells |
Clostridium sporogenes
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What are Bacillus megaterium?
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(1)aerobic (2) produce endospores (3) easy study under the light microscope
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NAME
is areobic and produce endospores |
Bacillus megaterium
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NAME
are easy to study under the light microscope |
Bacillus megaterium
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Name two ocular adjustments you have to make in order to avoid eye strain and headache associated w prolonged microscopic use
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adust the inerpulpllliary distance and the diapter reading to 65 to 40
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Why are microbes hard to see in wet preparations?
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bc they are moving around, making it harder to focus on one microbe for a long period of time
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How do you achieve subdued illumination when examining wet mounts?
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you would darken the background by using the condenser and the illuminator
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What organs of locomotion do you find in each of the living protozoans?
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(1)pseudopods (2)cilla (3)flagella
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Why do you need to use oil when viewing through the 100X objective?
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bc the light refracts the image to the viewer, the more power the objective lens has the easier you can view the image
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