Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
30 Cards in this Set
- Front
- Back
- 3rd side (hint)
|
Calculate magnification 10x × 10 = ? 40x × 10 = ? 100x × 10 = ? |
10x × 10 = 100x 40x × 10 = 400x 100x × 10 = 1000x |
Ocular × objective = magnification |
|
|
Pure culture define |
Isolated colony |
|
|
|
Colonial morphology define |
Whole colony. Function to describe shape, size and texture of colony |
|
|
|
Microscopic morphology define |
Individual cells, endospore stain, acid- fast stain, gram- stain |
|
|
|
Mixed culture define |
2 or more colonies |
|
|
|
Colony define |
Starts as single |
|
|
|
Shapes of bacteria Round = Rod shape = Spiral = |
Round = coccus Rod shape = bacillus Spiral = spirillum |
|
|
|
Arrangment of bacteria Pairs = Chains = Clusters = |
Pairs = diplococci Chains = streptococci Clusters = staphylococci |
|
|
|
Negative staining technique of the capsule |
Use ethier India ink or nigrosin for staining the background |
|
|
|
What are the 3 pathogens that poses a capsule |
1. Streptococcus 2. Klebsiella pneumoniae 3. Haemophilus influenza |
|
|
|
What are the functions of a capsule |
1. Resist phagocytosis 2. Increased virulence |
|
|
Whole colony and elevation ? |
|
|
|
|
Sterilization define |
Process of destroying all microbes including endospore viruses on inanimate objects |
Steam heat Auto clave Surgical equipment |
|
|
Disinfection define |
Kills microbe viruses and bacteria but not endospore on inanimate objects |
Bleach and vinegar |
|
|
Define sanitization |
Lowers the level of microbes to a safe level on inanimate objects |
Dishwasher |
|
|
Antisepsis define |
Reduces microbes on skin by washing hands |
Handwashing and surgical scrubs |
|
|
What are the Flagella types Amphitrichous = Lophotrichous = Peritrichous = Monotrichous = |
Amphitrichous = one or more on both ends Lophotrichous = one or more on one end Peritrichous = all over Monotrichous = one on one end |
|
|
|
What are the three different arrangements of endospores? 1. 2. 3. |
1. Central (middle) 2. Subterminal (near end) 3. Terminal (at end) |
|
|
|
What two pathogens posses endospores? |
Bacillus and clostridium |
|
|
|
What are the functions? Coarse focus knob: Fine focus knob: Abbe condenser knob: Iris diaphragm: |
Coarse focus knob: raising and lowering stage (10x only) Fine focus knob: Fine tune (40x - 100x) Abbe condenser knob: gather light Iris diaphragm: controller of light - right closed 10x - left open 10x |
|
|
|
What is the low power objective |
10x |
|
|
|
What is the high dry power? |
40x |
|
|
|
What is the oil immersion (reduces the scatter of light) |
100x |
|
|
|
Know gram stain Simple stain Streak stain Microscope parts |
Look at packet |
|
|
|
Elevated temperatures are ______; whereas lower temperatures are ______. |
1. Microbactericidal 2. Microbiostatic |
|
|
|
Moist heat vs dry heat |
Moist quicker to kill while dry takes longer at the same temperature |
|
|
|
Asepsis vs. Sepsis |
Asepsis is sterile technique Sepsis is invasion of microorganism tissue into bloodstream |
|
|
|
What is the function of a endospore |
Conditions are unfavorable so it is created to protect the DNA |
|
|
|
Epidemiology define |
Study of diseases and how they spread |
|
|
|
What are the steps a disease spreads? |
1. Susceptibility 2. Exposed 3. Infection 4. Recovered Incubation is from first contact to signs of symptoms Latency period is time between being exposed and showing symptoms |
|
