Micro Lab #1

Front 1

What type of media is Blood Agar?

Back 1

differential

Front 2

What type of media is Chocolate Agar?

Back 2

neither, it's the same as blood agar, except the blood is preheated to allow nutrients like hemin and NAD to escape from erythrocytes.

Front 3

What type of stuff is the Chocolate Agar for?

Back 3

bacteria w/ very specific nutritional needs, such as H. influenzae

Front 4

What type of media is MacConkey Agar?

Back 4

selective/ differential

Front 5

What kind of stuff grows on MacConkey Agar?

Back 5

ONLY gram negatives

Front 6

Bacteria that utilize lactose on MacConkey Agar produce what color colonies?

Back 6

pink

Front 7

Bacteria that are unable to utilize lactose as a sugar source grow what color colonies on the MacConkey Agar?

Back 7

clear to brownish

Front 8

MacConkey Agar is abbreviated:

Back 8

MAC

Front 9

Chocolate Agar is abbreviated:

Back 9

CHOC

Front 10

Blood Agar is abbreviated:

Back 10

BAP

Front 11

When deciding the appearance of the colonies, you should look for:

Back 11

color,
size,
shape,
elevation

Front 12

Other things to observe about colonies/plate include:

Back 12

odors,
hemolysis patterns,
discolorations

Front 13

Normal microbial flora are most prevalent where:

Back 13

oral cavity,
upper respiratory tract,
the colon,
the skin,
the lower genitourinary tract

Front 14

What body areas are normally free from microbes:

Back 14

blood,
cerebrospinal fluid,
deep tissues

Front 15

Those bacteria other than normal flora in/on the body are called:

Back 15

strict pathogens

Front 16

The appearance of the bacterial colonies on a plate is called:

Back 16

colony morphology

Front 17

Gram stain that binds to peptidoglycan:

Back 17

Crystal Violet

Front 18

permanently attaches CV to PPG

Back 18

Iodine

Front 19

removes the CV from the gram negative bacteria, leaving them "invisible"

Back 19

alcohol

Front 20

the red dye used to stain the gram negatives

Back 20

safranin

Front 21

Gram positives stain

Back 21

purple

Front 22

Gram negatives stain

Back 22

pink

Front 23

The control stain is made up of

Back 23

S. aureus and E. coli

Front 24

S. aureus is

Back 24

G+

Front 25

E. coli is

Back 25

G-

Front 26

The first step to prepare a gram stain is to get the slide ready by:

Back 26

Making 2 nickel sized circles on the slide, and labeling one end. Then, flame the inoculating loop to red hot, and briefly allow to cool. Place a very small drop of fresh tap water in a circle, and flame the loop again. Touch the colony gently with the loop and mix with water. Spread the mixture to the edges, and fan w/ hand or paper to allow the smear to air dry. Pick up the slide with a clothespin b/w the circles, and run through the flame 3-4 times. Allow slide to cool briefly.

Front 27

The next step is staining with:

Back 27

CRYSTAL VIOLET. wait 15 to 30 seconds, then gently rinse with water under the faucet.

Front 28

Then, drip ________ solution onto each circle and wait __________.

Back 28

IODINE: 15 to 30 seconds, rinse gently with water.

Front 29

Add ________ dropwise until the purple dye ___________.

Back 29

95% ETHANOL: running, then immediately rinse with water

Front 30

Drip ___________onto each circle and wait _________.

Back 30

SAFRANIN: 15 to 30 seconds, gently rinse, blot dry on fresh bibulous paper

Front 31

These bacteria are ALWAYS catalase +

Back 31

staphylococci

Front 32

These bacteria are ALWAYS catalase -

Back 32

streptococci

Front 33

The catalase test uses:

Back 33

hydrogen peroxide (H202)

Front 34

A positive catalase test is indicated by :

Back 34

bubbles!