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49 Cards in this Set
- Front
- Back
The purpose of the Catalase test
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To identify if organism makes enzyme catalase.
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Catalase test
Indicator/ Reagent |
Hydrogen-peroxide
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Medium for Catalase test |
None
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Catalase test results |
positive: bubbles
negative: no bubbles |
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Nitrate Reduction test purpose
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To show microbe reduce Nitrate to Nitrite, Nitrogen gas , Ammonia, or no reduction.
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Nitrate Reduction Test
Indicator/ Reagent |
Nitrate regents A and B if no nitrogen gas.
Zinc powder if no reduction. |
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Nitrate Reduction test
Medium |
Nitrate broth with Durham tube
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Nitrate Reduction test results
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Positive: with Nitrate A and B :Red
Negative: No change Positive with Zinc powder: colorless Negative with zinc powder: Red |
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IMViC test
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Indole test
MR test VP test Citrate test |
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IMViC test Media
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3 media: SIM deep, MRVP broth, Simmon citrate agar slant w/ Ph indicator
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Indole test purpose
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to test for motility, H2S and Indole production
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Indole test Indicator/ Reagent
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Kovac's reagent
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Indole test Medium
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SIM deep
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Indole test results
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Motility: + diffusion - no diffusion
H2S: + black and - not black Indole: Red ring on top - not red ring on top |
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Methyl-Red test ( MR) Purpose
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determine the use of glucose and Acid
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MR test Indicator/ Reagent
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Methyl-Red ( 8 drops)
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MR test Media
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MRVP broth
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MR test results
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Positive(Acidic) : Red- glucose user
Negative (Basic): Yellow- Not glucose user |
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Voges-Proskauer test Purpose
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determine the production of Acetoin
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V-P test Indicator/ Reagent
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Barritt's A (12 drops)
Barritt's B ( 4 drops) |
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V-P test Media
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MRVP broth
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V-P test Results
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Positive: Red- Acetoin
Negative: Yellow |
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Oxydase test Purpose
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to show microbe can use oxygen (oxydase)
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Oxydase test Indicator/ Reagent
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( tetranethyl-p- phenylenediamine)
TMPD |
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Medium of Oxydase test
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Filter paper
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Oxydase test results
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Positive: Purple
Negative: Colorless |
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Simmon's Citrate Test Purpose
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Identify the use of citrate as sole Carbon source
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Citrate Test
Indicator/ Reagent |
BROMOTHYMOL- BLUE
(ph indicator) |
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Citrate Test Media
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Simmon's citrate agar slant w/ ph indicator
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Citrate Test Results
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Positive : Royal-blue color
Negative: green |
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Decarboxylation Test Purpose
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To see bacteria can decarboxitate and produce the decarboxylase enzyme
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Decarboxylation Test
Indicator/ Reagent |
Bromcresol Purple
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Decarboxylation Test Media
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3 Media: with decarboxylase broth (arginine, lysine, ornithine)
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Decarboxylation Test Results
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Positive: Purple (basic)
Negative: Yellow (Acidic) |
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Deamination Test Purpose
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To see if organism able to deaminate(remove) Phenylalanine
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Deamination Test Indicator/Reagent
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Ferric-Chlorine (10-15 drops)
(FeCl 3) |
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Deamination Test Medium
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Phenylalanine agar slant
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Deamination Test Results
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Positive: Avocado green (dominase)
Negative: not avocado green |
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Urea Test Purpose
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To determine if microbe can break down Urea
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Urea Test Indicator/ Reagent
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Phenol-Red as ph indicator
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Urea Test Media
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Urea broth w/ph indicator
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Urea Test Results
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Positive: Hot pink ( Urease)
Negative: Yellow |
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Gelatin Hydrolysis Purpose
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to see the ability of the microbe to break down protein gelatin (gelatinase)
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Gelatin Hydrolysis
Indicator/ Reagent |
None
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Gelatin Hydrolysis Media
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Gelatin deep
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Gelatin Hydrolysis test Results
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Positive: Liquid gelatinase
Negative: Solid gelatinase |
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Bacteria that can grow on air
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Obligate aerobe
facultative anaerobe, and microaerophile |
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Bacteria that will grow in gas pack jar
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Obligate anaerobe, Facultative anaerobe
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Bacteria that can grow in candle jar
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Obligate anaerobe, Facultative anaerobe, microaerophile
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