Micro 140 Lab Practical Text

Front 1

How is the gram stain done? What are the reagents? Describe the gram morphology of an organism focused on a microscope

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Front 2

What is a spectrophotometer and what does it measure? Is the wavelength of light important? How is it used?

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Front 3

How are pour plates made? How are they counted? Calculate the dilution factor and determine the number of colony forming units in the original sample.

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Front 4

Interpret results on Mannitol Salt Agar (MSA)? How and when is this medium used? What is meant by the phrase "selective and differential?"

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Front 5

Remember yeasts?!

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Front 6

Remember the other fungi?

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Front 7

Interpret results on Blood Agar (BAP). Selective and differential?

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Front 8

How does one interpret a Kirby-Bauer (agar diffusion) antibiotic sensitivity test?

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Front 9

Interpret results on Eosin Methylene Blue (EMB) and MacConkey (Mac) plates. Selective and differential?

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Front 10

What are phototrophs? chemotrophs? autotrophs? heterotrophs? prokaryotes? eucaryotes? sporeformers? Have we ever worked with any of these? In what contexts?

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Front 11

Identify endospores on a microscope slide.

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Front 12

Remember photosynthesis? What were the principles involved in the experiment we did in class?

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Front 13

What do capsules look like? What is their function?

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Front 14

Which experiments investigated the oxygen requirments of microorganisms?

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Front 15

What kinds of physical and chemical agents did we investigate for their antibacterial activity? How were these experiments done?

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Front 16

How was the ELISA done and interpretted?

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Front 17

How was the handwashing experiment done and interpretted? What exactly was being measured in that experiment?

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Front 18

Phenol Red. Where and why and how did we use it throughout the semester?

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Front 19

Catalase. What is the importance of it and how did we look for it?

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Front 20

Can you identify and describe the function of the following microscope parts:eyepiece/stage/spring loaded slide holder/nosepiece/objectives/ coarse and fine adjustment/condenser/condenser adjustment knob/ diaphram/light source. What are some the important procedures to clean and take care of your microscape?

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Front 21

Can you read a pipette accurately? What do numbers like 5 in 1/10 or 1 in 1/100 mena? Can you use the "meniscus" appropriately?

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Front 22

What is a pour plate? What is considered a "countable" plate? How many ml = 1L How many nanograms in 1g? How many ml=1 cl?

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Front 23

Can you identify the results of an endospore stain and a capsule stain under the microscope?

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Front 24

Can you tell, IN GENERAL,a bacteria from a fungus from a yeast from a eucaryotic plant or cell using your microscope?

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Front 25

Can you read +/- sugar fermentation tubes? What are 3 common byproducts of fermentation? What groups of organisms normally use fermentation in their metabolism?

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Front 26

Could you name,describe,and "read" at least 5 different Differential Media?(including why are they used)

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Front 27

Can you group microorganisms into their "feeding" relationships? In our Photosynthetic Expt., can you explain what set up and what the changes with phenol red meant? Can you explain Photosynthesis?

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Front 28

What are aerobes,anarobes and microaerophiles? Can you describe and identify Thiglycollate patterns? What other test set up did we use for identifying anaerobes/aerobes? What are the enzymes available for aerobes to "detoxify" 02 reactions?

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Front 29

What does UV light do to cells? Can you describe the UV expt. in class(including the results)?

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Front 30

Can you identify the various hemolysis reactions of BAP(blood agar) For which bacterial groups was BAP most useful?

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Front 31

What are some additional tests foor identying species of Streptococcus and Staphylococcus? Can you identify the reactions?

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Front 32

What is the catalase test? How do you do it and read it?

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Front 33

What is the Kirby-Bauer test? What is it used for? How do you read it and interpret the results?

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