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38 Cards in this Set

  • Front
  • Back

Types of Media

Differential


Selective


General

Differential

media that may grow different types of bacteria.


eg. macConkey, Eosin MTB

Selective

used to grow only desired bacteria and to inhibit the growth of unwanted bacteria. (e.g Mannitol Salt, Sabouraud-Dextrose)

General

most bacteria will grow. eg. nutrient agar

Plates

use petri dishes


fill just enough to cover the bottom of the dish.


45 degrees

Deeps

Approximately 10-20 ml of media is poured into a test tube and capped.

Slants

same as deeps, but media is cooled at 45 degrees

Broth

uses same ingredients except for agar. media stays in liquid form

Complex

grows most heterotrophic organisms, composition varies from batch to batch

Defined

grows specific heterotrophs and often mandatory for chemoautotrophs.


exact composition of media

Selective media

suppresses unwanted microbes

Differential

Distinguishes colonies of specific microbes from others

Enrichment

similar to selective but designed to increase # of desired microbes to a detectable level.

Reducing

Reduces oxygen concentration. required for obligate anaerobes

Blood Agar

rich, nonselective, differential


5% sheep blood to complex media


other bacteria release hemolysins (enzymes that break down RBC

Beta-Hemolytic

lyse RBC and degrade hemoglobin



Alpha-hemolytic

lyse RBC, do not degrade hemoglobin

Gamma-hemolytic

no harm to RBC

Eosin Methylene Blue(EMB)

selective and differential media

comobo of dyes, eosin, and MTB


only allow Gram (-) to grow


Gram (-) bacteria that ferment lactose

produce acid, blue-black appearance

Gram (-) bacteria that ferment lactose rapidly

very distinct green-metallic sheen

3) MacConkey Agar (MAC)

differential plating medium selective for gram -


detect and isolate enteric bacteria

4) Mannitol Salt Agar(MSA)

highly selective medium for staphylococci.


7.5% of NaCl



Pathogenic Staphylococci

ferment mannitol>lower ph> red turns yellow

Non-pathogenic Staphylococci

do not ferment mannitol>ph same> stays pink

Trypticase Soy Agar (TSA)

work horse


non-selective


non-differential medium, has proteins and dextrose

Sabouraud Dextrose Agar (SDA)

selective, growth of fungi (yeast and molds)


due to low ph

Phenylethanol Agar (PEA)

selective for gram +


phenylethanol is bacteriostatic agent


good to grow staph or strept

Triple Sugar Iron Agar (TSI)

differentiation of gram - enteric bacilli


Dextrose, sucrose, and lactose (all 1%)


iron source

top and bottom all yellow

ferment all sugars

top red, bottom yellow

only dextrose fermented

top and bottom red

non-fermenter

hydrogen sulfide production

black precipitate

Gas production

looks like cracks

Brilliant Green Agar (BG)

highly selective


isolation of salmonella

E. coli

lactose fermenter(yellow-green colonies)

Salmonella spp.

non-lactose fermenter(red colonies)

Nutrient Agar(NA)

work horse


non-selective and non differential