Media And Biochemical Tests

Front 1

Which tests use SIM's media?

Back 1

Indole test, H2S production, Motility test

Front 2

Describe how to perform an Indole test

Back 2

Inoculate sim's media, incubate, then add Kovac's reagent

Front 3

What is the distinctive media in an Indole test?

Back 3

trytophan

Front 4

What does a positive reaction of an Indole test look like?

Back 4

Red color at top

Front 5

What is the distinctive media in the H2S test?

Back 5

iron, sulfur

Front 6

What is a result for H2S test?

Back 6

Black precipate

Front 7

What does a positive result for a motility test look like?

Back 7

Growth away from stab, or turbidity

Front 8

How do you perform a nitrate test?

Back 8

Inoculate broth, intubate, add reactant

Front 9

What is the important parts of the media in a nitrate test?

Back 9

beef extract, peptone, potassium nitrate

Front 10

What is a positive results for a nitrate test?

Back 10

Red

Front 11

What is a result for H2S test?

Back 11

Black precipate

Front 12

What does a positive result for a motility test look like?

Back 12

Growth away from stab, or turbidity

Front 13

How do you perform a nitrate test?

Back 13

Inoculate broth, intubate, add reactant

Front 14

What is the important parts of the media in a nitrate test?

Back 14

beef extract, peptone, potassium nitrate

Front 15

What is a positive results for a nitrate test?

Back 15

Red

Front 16

What does the urea hydrolysis test for?

Back 16

The ability to hydrolyze urea to make ammonium

Front 17

What is the distinctive media for a urea hydrolysis test?

Back 17

Urea broth, phenol red, yeast extract

Front 18

What is a positive result for a urea hydrolysis test?

Back 18

Bright pink

Front 19

How do you perform an Oxidase test?

Back 19

Inoculate TSA w/organism, incubate and add oxidase reagent
OR
add 1 drop tap water to oxi-strip and place 1 to colonies on applicator stick, read after 15 secs

Front 20

What does does the Oxidase test?

Back 20

ability to use cytochrome oxidase

Front 21

What is a positive result for the Oxidase test?

Back 21

Colonies turn purple when exposed to oxidase reagent

Front 22

How do you perform a Catalase test?

Back 22

streak inoculate a TSA plate, intubate, add hydrogen peroxide

Front 23

What is a positive result of the Catalase test?

Back 23

If colonies bubble

Front 24

What do the Sugar Fermentations test?

Back 24

Ability to ferment sugar

Front 25

What is distinctive in the media for Sugar Fermentation test?

Back 25

Sugar, phenol red, durham tube

Front 26

What does a positive Sugar Fermentation test look like?

Back 26

Turned to yellow, and/or gas in durham tube

Front 27

How do you perform the Triple Sugar Iron Agar test?

Back 27

Stab slant and streak top

Front 28

What does the Triple Sugar Iron Agar test?

Back 28

Ability to ferment glucose and lactose/sucrose

Front 29

What are the distinctive media in Triple Sugar Iron Agar?

Back 29

phenol red, sugar, agar, ferrous sulfate

Front 30

What do the results look like for Triple Sugar Iron Agar?

Back 30

butt yellow/slant red glucose only KA
everything yellow, glucose + AA
no change nothing K/NC
black precipate sulfur H2S
cracks produced CO2 gas

Front 31

How do you perform the Methyl Red/Vogues Proskaver test?

Back 31

Inoculate, incubate and add reactant

Front 32

What does the MR/VP test?

Back 32

used to differentiate between species of enterobacteriacea. MR - acid from glucose VP - test for acetoin

Front 33

What does a positive MR and positive VP test look like?

Back 33

MR - result is red, pH is below 5
VP - result is red after addition of VP reagents

Front 34

How do you perform a Citrate test?

Back 34

Inoculate green slant and ferment

Front 35

What does Citrate test?

Back 35

if bacteria uses citrate as sole carbon source and nitrogen as sole source

Front 36

What are the distinctive media in a Citrate test?

Back 36

simmon's citrate agar, pH indicator

Front 37

What does a positive Citrate test look like?

Back 37

Media turns blue

Front 38

How do you perform a Starch Hydrolysis test?

Back 38

Inoculate plate, incubate, flood plate with iodine

Front 39

What does the Starch Hydrolysis test?

Back 39

Ability of bacteria to hydrolyze starch with amylase

Front 40

What does a positive Starch Hydrolysis test look like?

Back 40

Clear ring around colonies

Front 41

What is in distinctive media in the Litmus Milk test?

Back 41

skim milk, pH indicator

Front 42

What are the 4 things the Litmus Milk tests?

Back 42

Lactose fermentation, reduction of litmus, curd formation and peptonization

Front 43

What does a pink test mean? LM

Back 43

lactose fermenation

Front 44

What does a white test mean? LM

Back 44

reduced litmus

Front 45

What does a purple test mean? LM

Back 45

oxidized litmus

Front 46

What does a brownish test mean? LM

Back 46

peptonization

Front 47

What does a blue test mean? LM

Back 47

digestion of casein

Front 48

What does a purple or blue ring of color mean? LM

Back 48

alkaline reaction

Front 49

What does clumping mean? LM

Back 49

accumulation of acid and to create casein clump

Front 50

What does a brownish test mean? LM

Back 50

peptonization

Front 51

What does a blue test mean? LM

Back 51

digestion of casein

Front 52

What does a purple or blue ring of color mean? LM

Back 52

alkaline reaction

Front 53

What does clumping mean? LM

Back 53

accumulation of acid and to create casein clump