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9 Cards in this Set
- Front
- Back
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what are the 10 steps for tissue preparation for LIGHT MICROSCOPY?
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1. obtain "living" tissue
2. coagulate proteins and harden tissue 3. Dehydrate with alcohol 4. clear with xylene to remove alcohol 5. infiltrate with partial paraffin 6. embed with straight paraffin 7. section tissue with microtome 8. mount and remove paraffin with xylene 9. stain tissue 10. mount with cover slip and balsam |
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what is the size the the tissue shavings for light microscopy?
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7nm
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what is the size of the tissue shavings for electron microscopy
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40nm
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hematoxylin
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stains acid components blue
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eosin
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stains basic components pink
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electron microscopy tissue prep
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1. fixation/dehydrations steps same as light M
2. embed tissue in hard resin 3. section tissue shavings 4. stain tissue with METAL |
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how is tissue visualized in electron microscopy?
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light source consists of a stream of electrons which pass down the microscope and are focused by magnetic lenses. the pass through those parts of the tussle not containing heavy metals and strike a phosphorescent screen when ere an image can be observed.
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histochemistry
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use of special staining techniques to detect the presence of specific chemical entities (i.e. lipids, carbohydrates, enzymes)
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Periodic acid-schiff
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used to detect the presence of carbohydrate
can find disease process in kidneys |
