Mcb 112 Midterm 2 (Carbon Fixation)

Front 1

What are 3 light harvesting systems?

Back 1

1. LH1 & LH2 of purple bacteria (flower-looking thing)

2. Chlorosomes of green sulfur bacteria

3. Phycobilins in cyanobacteria (hand fan-like)

Front 2

How is CO2 converted to carbs?

Back 2

by dark reactions--> ATP and NADH/NADPH made by phototrophy reduce CO2

Front 3

What are the 3 carbon fixation pathways?

Back 3

1. Calvin cycle

2. Reverse TCA cycle

3. 3- Hydroxypropionate cycle

Front 4

How did they discover use of Calvin cycle

Back 4

used lollipop device. 14CO2 injected & samples removed to see where 14C went

Used TLC & autoradiography

Front 5

Need to knows on TCA Cycle

Back 5

1. carboxylation

2. Reduction

3. Regeneration

- Need NADH/NAPH & ATP

-- Uses RuBisCO & PRK (KEY RXNS)

Front 6

What does RuBisCO do?

Back 6

2 competing rxns:

1. uses O2 to produce 1 PGA & 1 PPG

2.

Front 7

What is 1 carbon concentration mechanism?

Back 7

1. carboxysomes of cyanobacteria

protein shell tightly controls what chemicals pass into/out of compartment

Front 8

What is another carbon concentrating mechanism?

Back 8

Inducible high affinity transporters maximize uptake of CO2 and HCO3-

Front 9

What drives reverse TCA cycle?

Back 9

ATP and reduced Fd from light dependent rxns

There are multiple entry points for CO2

If you see ATP citrate lyase, automatically think reverse TCA!!!