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1 Cards in this Set

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Meselson-Stahl experiment
1. Used to determine that DNA replication is semi-conservative

2. Protocol:
(a) Grow E.coli in "heavy" ammonia, and then switch to normal ammonia

(b) Harvest aliquots as a function of time, and isolate DNA

(c) Separate by DNA density with density gradient centrifugation

(3) Showed fractions that correspond to semi-conservative scheme