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7 Cards in this Set
- Front
- Back
01: what is one property of the AA code?
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DEGENERACY: several codons can each code for one shared amino acid.
SYNONYMS: codons for the same AA. will have similar tRNAs |
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02: which directions are codons and anticodons read?
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CODON: 5'->3'
ANTICODON: 3'->5' |
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03: What is "wobble"
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anticodon base can pair w/ alternate bases in codon, especially variation in THIRD base of the codon.
ie U at the wobble position can pair with an A or G in the third base of the mRNA one tRNA recognizes all codons for one AA |
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04: how was the RNA-AA code cracked?
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using 3 base-long sections of RNA, put into conditions w/ ribosome pieces. if incorrect AA, ribosome complex will not form.
then add all tRNA's to ensure a complex will form. make sure one tRNA is hot. if complex forms (as seen on filter paper over a vacuum that sucks ) filter will be radioactive, and has your tRNA in there. ( free tRNA will pass through while ribosomes stick) |
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05: rules of the genetic code
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1: codons read 5'->3'
2: codons do not overlap or form gaps 3: the reading frame is fixed |
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06: kinds of point mutations
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supressor: stop mutations (make an incomplete chain)
intergenic (no effect) MISSENSE: changes one codon to another putting in wrong AA FRAMESHIFT: add 1 or 2 bases, drastically altering protein |
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07: is the genetic code universal?
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mostly.
some AA's were expanded, new stop codons added. |