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9 Cards in this Set
- Front
- Back
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What needs to be done before ABX susceptibility? |
Form cultures, identify organism, susceptibility test |
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What are some types of ABX susceptibility testing? |
Broth dilution Antimicrobial Gradient Disk diffusion |
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What is the broth dilution test? |
Innoculate same amount of a bacterial isolate into dilutions of antibiotic Lowest dilution that the bacteria doesn't grow in is the Minimum Inhibitory Concentration Typically done in 96 well microtitre plates |
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What is the antimicrobial gradient method? |
MIC is determined by the intersection of the organism growth with the strip as measured using the scale on the strip |
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What is the disk diffusion test? |
Measure radius, group as resistant/intermediate/susceptible |
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Why are different types of bacteria more effectively treated by different types of ABX |
Due to intrinsic and acquired resistances |
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What are intrinsic resistances in bacteria? |
Occur naturally Chromosomally encoded Reduced permeability to ABX ABX efflux |
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What are acquired resistances in bacteria? |
Results from a mutation in the DNA OR acquisition of new DNA that carries resistance Changes in ABX targets Modification of targets Direct modification of the ABX |
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