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14 Cards in this Set
- Front
- Back
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KNOW WHAT G COUPLED RECEPTOR PROTEIN LOOKS LIKE! (literally?) |
(see keynote) DO NOT FORGET TO |
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Simliar to how EGF receptor protein has one transmembrane domain, how many does G protein coupled receptor (GPCR) have? |
Seven transmembrane domains! |
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How does GPCR send its signal? (What protein is associated with the protein? (3 subunits, all different- alpha, beta, gamma)) This (Het?) is the on/off switch- Which is off and which is on? |
(Heterotrimeric) Off is GDP bound/ON is GTP bound |
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When it's on- what does ... allow? |
You make GDP come off G protein which allows GTP to bind to it |
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Activation is an exchange of _____ displacing _____; to turn it off you have to go back to ______ which is not an exchange |
GTP, GDP, GDP |
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How does it turn off? What cuts off what? How does this work-- GTPase of ______ converts ____ to _____ and releases one ______ into the environment making it an exchange to activate GTPase (RAS activation cycle works same way- GDP off, GTP on,e xchanges to activate and cleaves to inactivate by GTPase) |
GTPase cuts off one of the phosphates alpha subunit, GTP to GDP, releases one phosphate |
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What activates protein kinase A? (also called cyclic A dependent protein) |
Cyclic AMP |
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PKA stimulates _______ factors that bind to _______ and stimulate ________ expression |
transcription DNA gene |
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What subunits does cAMP have? (2 each of 2 kinds) Which keeps which inactive? What happens to them when you bind cAMP and what does that release? Once released what does that substance do to the other proteins? |
regulatory and catalytic regulatory keeps catalytic inactive when you bind they come apart, releases active catalytic to phosphorylate other proteins |
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What is PIP2? What can you get off PIP2? (specifically) KNOW ABOUT THESE MOLECULES SEE PLC HYDROLYSIS SLIDE |
3 other signaling molecules PIP3 and DAG |
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What domains does PIP3 have? What do they do? If protein has these xx domains when PIP3 is generated what can it bind to? |
the PH domains; they recognize PIP3 can bind to PIP3 and AKT |
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The kinase controlling survival pathway has a PH domain- what does that do for AKT? What does it stimulate? |
activates AKT/stimulates downstream survival pathway (see chart- PKC by disoglycerol ??) |
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When PIP3 is released downstream of a G protein what does it release, from what, through what?? What does it operate when that store is depleated (channels in ____)? |
releases calcium from intercellular stores through a channel channels in plasmam membrane |
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This process above is one of the main ways that ________ cells get calcium from the outside, since they don't have voltage regulated channels Also: PIP3 is one signal off PIP2 molecule PIP3 receptor is the only kind of store for the calcium receptor- the IP3 is a channel, when it binds it's only IP3 (IP or PIP?) and lets calcium out of the stores |
epithelial |
