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12 Cards in this Set
- Front
- Back
how is DNA prematurely terminated?
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addition of dideoxynucleoside triphosphates (ddNTPs)
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what do you call when dideoxynucleotides have hydrogen in 3' position and stop DNA synthesis when added to a growing chain?
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chain terminating nucleotides
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what are the methods called (2 diff names)
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sanger method, dideoxy method
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4 _______ taking taking place in 4_______
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separate DNA polymerization reactions, reaction tubes.
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what's added after DNA tamplate to be sequenced were placed in tubes?
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four types of dNTPs as building blocks of new sequences and DNA polymerase to catalyze reaction.
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primer has...
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radioactive or fluorescent label on 5' end for visualization
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4 ddNTPs
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ddATP, ddGTP, ddCTP, ddTTP
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ratio
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1ddNTP/50 dNTP
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reactions tubes will be separated on
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denaturing polyacrylamide gel and detected by either autoradiography or fluorescence.
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what detects color of fragment when electrophoresed thru polyacrylamide gel?
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laser beam
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data print out is called
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electropherogram
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clusters on the phylogenetic tree are called
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haplogroups, which consist of various haplotypes that are similar and share a common ancestor.
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