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20 Cards in this Set

  • Front
  • Back
Lactose Fermenter
Oxidase negative
TSI A/A (LF)
No Hs2 production
Indole positive
E. coli
What does oxidase positive even mean?
OX+ normally means that the bacterium contains cytochrome c oxidase and can therefore utilize oxygen for energy production with an electron transfer chain.
LF
Oxidase negative
TSI A/A (LF)
No H2S
Indole negative
Klebsiella pneumonia
LF
oxidase negative
TSI A(K)/A
H2S Produced
Indole negative most of the time
Citrobacter freundii

Citrobacter is a genus of Gram-negative coliform bacteria in the Enterobacteriaceae family.
NLF
oxidase negative
TSI K/A
H2S production
No indole formation

Swarming colonies
Proteus mirabilis

Proteus mirabilis is a Gram-negative, facultatively anaerobic bacterium. It shows swarming motility, and urease activity. P. mirabilis causes 90% of all 'Proteus' infections in humans.
NLF
oxidase negative
TSI A(K)/A (NLF)
H2S Production
Indole Positive

Swarming colonies
Proteus vulgaris


Proteus vulgaris is a rod-shaped, Gram negative bacterium that inhabits the intestinal tracts of humans and animals. It can be found in soil, water and fecal matter. It is grouped with the enterobacteriaceae and is an opportunistic pathogen of humans. It is known to cause urinary tract infections and wound infections.
NLF
Oxidase negative
TSI K/A
H2S production
Few indole positive
Salmonella enteritis
NLF
oxidase negative
A (K)/A
no H2S
few indole postiive

Some have red/orange pigment
Serratia marcesens

Serratia marcescens is a species of Gram-negative, rod-shaped bacterium in the family Enterobacteriaceae. A human pathogen, S. marcescens is involved in nosocomial infections, particularly catheter-associated bacteremia, urinary tract infections and wound infections,[1][2] and is responsible for 1.4% of nosocomial bacteremia cases in the United States.[3] It is commonly found in the respiratory and urinary tracts of hospitalized adults and in the gastrointestinal system of children.
NLF
oxidase positive (only one among the gram negative rods we studied in lab!)
TSI K/K
No H2S
Indole negative

Metallic/green sheen
Pseudomonas aeruginosa
NLF
oxidase negative
K/K
No H2S Production
Indole negative
Stenotrophomonas maltophilia

S. maltophilia frequently colonizes breathing tubes such as endotracheal or tracheostomy tubes, the respiratory tract and indwelling urinary catheters.
Gram negative coccobacilli
Small gram negative bacilli
suggestive of Hemophilus, fastidious and anaerobic organisms. Pseudomonas may occur as larger coccobacilli
Gram negative bacilli
Enteric gram negative rods

* Enterobacteriaceae (=enteric gram-negative rods, enteric bacteria, coliform bacteria): a large, heterogeneous group of gram-negative rods
* Natural habitat is the intestinal tract of humans and animals
* Some enteric organisms (E.coli) are part of the normal flora and incidentally cause disease, while others (salmonellae, shigellae) are regularly pathogenic for human
Gram negative anaerobes
Bacteroides
Fusobacterium
Gram negative aerobic cocci
Neisseria
Gram negative Aerobic bacilli/coccobacilli

Do grow on MacConkey agar, ferment lactose
LF: Enterobacter
E Coli
Citrobacter
Klebsiella
Gram negative Aerobic bacilli/coccobacilli

Do grow on MacConkey agar, do NOT ferment lactose, do NOT ferment glucose
Oxidase positive: pseudomonas, burkholderia

Oxidase negative: Acinetobacter
Stenotrophomonas
Aerobic bacilli/coccobacilli

Do grow on MacConkey agar, DO NOT ferment lactose, ferment glucose
Salmonella
Shigella
Proteus
Providencia
Mogranella
others
Gram negative bacilli/coccobacilli

Do NOT grow on MacConkey agar
Haemophilus

Campylobacter/Legionella/Bordetella require special growth media
Gram negative cocci
Probable anaerobe
Gram negative intracellular diplococci
Probably neisseria or branhamella