Lab Quiz 5

Front 1

MAC

Back 1

MacConkey Agar

Front 2

Purpose of MAC

Back 2

Isolation of gram negative enterics

Front 3

Category of MAC

Back 3

Selective and differential

Front 4

Inoculation of MAC

Back 4

quadrant streak

Front 5

What is the special inoculation technique used only for the urine test?

Back 5

semiquantitative method using the volumetric loop

Front 6

What are the special ingredients for MAC?

Back 6

SA=Bile salts and crystal violet

DA=lactose

pH indicator=neutral red

Front 7

Reading criteria for MAC

Back 7

purple growth= (+)lactose fermentation

colorless growth=(-) lactose fermentation

Front 8

MSA

Back 8

Mannitol Salt Agar

Front 9

Purpose of MSA

Back 9

isolates and differentiates halotolerant species

Front 10

Category of MSA

Back 10

selective and differential

Front 11

Inoculation technique for MSA

Back 11

quadrant streak

Front 12

Special ingredients for MSA-mannitol salt agar

Back 12

SA=7.5 % NaCI

DA= Mannitol

pH indicator=phenol red

Front 13

Reading criteria for MSA-mannitol salt agar

Back 13

1.growth=halotolerance, medium is lemon-yellow=mannitol fermentation (+)

2. growth=halotolerance, medium is pink =mannitol fermentation (-)

3. No growth=halotolerant (-)

Front 14

reading of the urine streak

Back 14

min of 100k CFU determines an active UTI

Front 15

OCD

Back 15

original cell density

Front 16

Calculation of OCD

Back 16

CFU's divided by the loop volume

ex: CFU/loop volume- 75 CFU/0.001 mL=OCD

Front 17

Kirby Bauer Method

Back 17

bacterial lawn technique, a type of culture and sensitivity test

Front 18

BA

Back 18

Blood Agar

Front 19

Purpose of BA

Back 19

test for hemolytic activity

Front 20

Category of BA

Back 20

Differential

Front 21

Inoculation of BA

Back 21

quadrant streak method

Front 22

Preparation of BA

Back 22

autoclaved, cooled, blood then added

Front 23

SIM

Back 23

sulfide indole motility medium

Front 24

purpose of SIM

Back 24

screening of H2S, indole production, and motility

Front 25

category of SIM

Back 25

biochemical

Front 26

incoluation of SIM

Back 26

stab

Front 27

reagent of SIM

Back 27

Kovac's reagent

Front 28

UB

Back 28

urea broth

Front 29

purpose of UB

Back 29

production of exoenzyme urease

Front 30

category of UB

Back 30

biochemical

Front 31

inoculation of UB

Back 31

dip and swirl

Front 32

pH indicator of UB

Back 32

Phenol red

Front 33

gram positive coccus-resident of digestive tract-assoc with UTI-can lead to endocartis-abdominal infections-at risk with long term hospitalization and catheters.

Back 33

Enterococcus faecalis

Front 34

gram negative rod, coliform group, produces vitamins k and B complex, montezumas revenge

Back 34

Escherichia coli

Front 35

gram negative rod, lives in soil and water, organic matter decomposer, highly motile, opportunist of UG tract, nosocomial infections, resistant to ampicillan and cephalosporin, suseptible to trimethoprim

Back 35

Proteus vulgaris

Front 36

gram positve cocci in grape like clusters, wide spread assoc with skin and mucus membranes, MRSA, VRSA

Back 36

Staphylococcus aureus

Front 37

on the skin opportunist, strains produce a slime layer which allow attachment to certain hospital apparatuses, infections at site of prosthetic implantation

Back 37

Staphylococcus epidermidis

Front 38

leading cause of UTI's-young women more susceptible to colonization-hydrolyzes urea to produce ammonia allowing growth in an acidic environment, spread through sexual intercourse

Back 38

Staphylococcus saprophyticus